Administration of antibiotics can cause dysbiosis in fish gut

Kim, Ahran; Kim, Nameun; Roh, Heyong Jin; Chun, Won-Kyong; Ho, Diem Tho; Lee, Yoonhang; Kim, Do-Hyung

doi:10.1016/j.aquaculture.2019.734330

Cited by 48 publications

(30 citation statements)

References 45 publications

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“…Vibrio was known as opportunistic bacteria [53]. Recent study showed that the antibiotic-induced reduction of bacterial richness and diversity in fish gut increased the dominance of opportunistic Vibrio in the gut environment [54]. In line with our study, microbial richness was found significant lower in the toxic puffer gut environment, where the Vibrio was found centered at the bacteria co-occurrence network with significantly high relative abundance than that in the non-toxic gut environment.…”

Section: Metabolic Pathways Relating To Ttx Biosynthesissupporting

confidence: 89%

Puffer Fish Gut Microbiota Studies Revealed Unique Bacterial Co-Occurrence Patterns and New Insights on Tetrodotoxin Producers

Tian

Lai

et al. 2020

Marine Drugs

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Tetrodotoxin (TTX) is a potent neurotoxin isolated mainly from toxic puffer fish. To date, the TTX biosynthetic mechanism inside its hosts remains unresolved. Here, we hypothesize the TTX synthesis relies on the host gut microbiota, including the neglected non-culturable bacteria. In these studies, we collected the gut contents from 5 puffer fish species of the genus Takifugu including one suspected hybrid species for gut microbiota study by 16S rRNA amplicon metagenomics approach. Their gut samples were divided into toxic and non-toxic groups based on the TTX concentrations in the livers detected by LC-MS/MS. Bacterial diversity studies showed that gut microbiota structures were significantly different between toxic and non-toxic species. Vibrio and Cyanobacteria centered at the gut bacterial co-occurrence network, suggesting their importance in TTX biosynthesis. The results of PICRUSt2 metagenomic prediction and gene set enrichment analysis provided new support of arginine-precursor required in TTX biosynthesis. This is the first study to profile the gut microbiota in toxic and non-toxic puffer fish species by 16S rRNA amplicon metagenomic approach, defining significant microbial co-occurrence patterns in their gut environment. Our data supported the proposed biosynthesis of TTX inside the hosts by their gut bacterial symbionts using arginine as a precursor.

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Section: Metabolic Pathways Relating To Ttx Biosynthesissupporting

confidence: 89%

Puffer Fish Gut Microbiota Studies Revealed Unique Bacterial Co-Occurrence Patterns and New Insights on Tetrodotoxin Producers

Tian

Lai

et al. 2020

Marine Drugs

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“…On the contrary, the effect of intramuscular antibiotic administration on the microbiome is less well investigated. Correlation between intramuscular antibiotic use and dysbiosis has been reported in sh 46 , gorillas 47 , humans 97 , and pigs 48,98 shifts of the Firmicutes/Bacteroidetes ratio following treatment (length of the treatment not reported) 98 . The effects of intramuscular administration of neomycin (aminoglycoside class) on the gut microbiota have to our knowledge not been investigated.…”

Section: Discussionmentioning

confidence: 99%

“…In livestock production, intramuscular (IM) antibiotic treatment is preferred over oral antibiotic treatment, as it is thought to contribute less to AMR than oral antibiotic treatment [41][42][43] . Extensive evidence exists on dysbiosis as a consequence of oral antibiotic treatment on the gut microbiome 22,[35][36][37][38]44,45 , whereas only a handful of studies report the effects of intramuscular (IM) antibiotic treatment on the gut microbial community [46][47][48] , and none, to our knowledge have studied the use of IM neomycin treatment.…”

Section: Introductionmentioning

confidence: 99%

Community composition and development of the post-weaning piglet gut microbiome

Gaio

DeMaere

Anantanawat

et al. 2020

Preprint

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BackgroundEarly weaning and intensive farming practices predispose piglets to the development of infectious and often lethal diseases, against which antibiotics are used. Besides contributing to the build-up of antimicrobial resistance, antibiotics are known to modulate the gut microbial composition. Studies have previously investigated the effects of probiotics as alternatives to antibiotic treatment for the prevention of post-weaning diarrhea. In order to describe the post-weaning gut microbiota, and the effects of two probiotics formulations and of intramuscular antibiotic treatment on the gut microbiota, we processed over 800 faecal time-series samples from 126 piglets and 42 sows, generating over 8Tbp of metagenomic shotgun sequence data. Here we describe the animal trial procedures, the generation of our metagenomic dataset and the analysis of the microbial community composition using a phylogenetic framework.ResultsFactors such as age, litter effects, and breed, by significantly correlating with gut microbial community shifts, can be major confounding factors in the assessment of treatment effects. Intramuscular antibiotic treatment and probiotic treatments were found to correlate with alpha and beta diversity, as well as with a transient establishment of Mollicutes and Lactobacillales, respectively. We found the abundance of certain taxa to correlate with weight gain.ConclusionsOur findings demonstrate that breed, litter, and age, are important contributors to variation in the community composition, and that treatment effects of the antibiotic and probiotic treatments were subtle, while host age was the dominant factor in shaping the gut microbiota of piglets after weaning. The current study shows, by means of a phylogenetic diversity framework, that the post-weaning pig gut microbiome appears to follow a highly structured developmental program with characteristic post-weaning changes that can distinguish hosts that were born as little as two days apart in the second month of life.

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“…The longterm exposure (8 weeks) of fish to NFLX, can reduce the gut microbiome that can negatively affect the fish physiological status through enzymatic activity reduction thus exhibit less growth than other groups. The results could be supported by Carlson et al, 2015;Carlson et al, 2017;Zhou et al, 2018;Kim et al, 2019). Dibner and Richards (2005) and Reda et al (2013) who reported significant enhancements in final body weight (g), WG (g), and a significant decrease in FCR of fish fed oxytetracycline in diet when compared to the control.…”

Section: Discussionmentioning

confidence: 71%