Administration of a Salmonella Enteritidis ΔhilAssrAfliG strain by coarse spray to newly hatched broilers reduces colonization and shedding of a Salmonella Enteritidis challenge strain

Cort, W. De; Haesebrouck, Freddy; Ducatelle, Richard; Immerseel, Filip Van

doi:10.3382/ps/peu018

Cited by 17 publications

(15 citation statements)

References 19 publications

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“…Both Salmonella counts and the inflammatory response confirmed that aerosol vaccination induced a greater systemic immune response. However, unlike in previous studies [ 10 – 12 ], the aerosol vaccination was less protective in the cecum than the oral vaccination. It is likely that multiple sites including the digestive tract, respiratory tract or conjunctiva are stimulated during aerosol vaccination which results in a systemic immune response.…”

Section: Discussioncontrasting

confidence: 91%

“…Aerosol vaccination is an efficient alternative to oral vaccination [ 10 – 12 ]. Another alternative increasing the vaccine’s potential is the administration of a live Salmonella vaccine together with probiotics or competitive exclusion products [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

“…Rapid vaccination soon after hatching can be achieved by spraying. Although there are several reports on aerosol administration of live Salmonella vaccines to chickens [ 10 – 12 ], there is no data on the long term protection following this mode of vaccine administration. Moreover, chickens vaccinated by aerosol in these studies were challenged orally and although oral challenge test the induction of gut immunity, it may not be sufficient for the characterization of systemic immunity.…”

Section: Introductionmentioning

confidence: 99%

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Immune protection of chickens conferred by a vaccine consisting of attenuated strains of Salmonella Enteritidis, Typhimurium and Infantis

Varmužová

Faldynová

Elsheimer-Matulova

et al. 2016

Vet Res

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The colonization of poultry with different Salmonella enterica serovars poses an issue throughout the world. In this study we therefore tested the efficacy of a vaccine consisting of attenuated strains of Salmonella enterica serovars Enteritidis, Typhimurium and Infantis against challenge with the same serovars and with S. Agona, Dublin and Hadar. We tested oral and aerosol administration of the vaccine, with or without co-administration of cecal microbiota from adult hens. The protective effect was determined by bacterial counts of the challenge strains up to week 18 of life and by characterizing the immune response using real-time PCR specific for 16 different genes. We have shown that a vaccine consisting of attenuated S. Enteritidis, S. Typhimurium and S. Infantis protected chickens against challenge with the wild type strains of the same serovars and partially protected chickens also against challenge with isolates belonging to serovars Dublin or Hadar. Aerosol vaccination was more effective at inducing systemic immunity whilst oral vaccination stimulated a local immune response in the gut. Co-administration of cecal microbiota increased the protectiveness in the intestinal tract but slightly decreased the systemic immune response. Adjusting the vaccine composition and changing the administration route therefore affects vaccine efficacy.Electronic supplementary materialThe online version of this article (doi:10.1186/s13567-016-0371-8) contains supplementary material, which is available to authorized users.

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Section: Discussioncontrasting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Immune protection of chickens conferred by a vaccine consisting of attenuated strains of Salmonella Enteritidis, Typhimurium and Infantis

Varmužová

Faldynová

Elsheimer-Matulova

et al. 2016

Vet Res

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“…The non- inoculated, susceptible sentinel birds mirror the animals naturally infected by the oral uptake of resistant bacteria from their surrounding environment after they were shed by the seeder birds. In the course of this, a variety of different ratios is known and a ratio of 1:5 seeder- to sentinel birds is more often described for the inoculation with Enterobacteriaceae (Methner et al, 2011; de Cort et al, 2015; Kilroy et al, 2015). Using this relation led to a complete colonization of all birds within our group.…”

Section: Discussionmentioning

confidence: 99%

Low Dose Colonization of Broiler Chickens With ESBL-/AmpC- Producing Escherichia coli in a Seeder-Bird Model Independent of Antimicrobial Selection Pressure

et al. 2019

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Extended-spectrum beta-lactamase- (ESBL-) and AmpC beta-lactamase- (AmpC-) producing Enterobacteriaceae pose a risk for both human and animal health. For livestock, highest prevalences have been reported in broiler chickens, which are therefore considered as a reservoir of multidrug-resistant bacteria. The possibility of transfer to humans either by a close contact to colonized broiler flocks or through contaminated retail meat results in the necessity to develop intervention measures for the entire broiler production chain. In this regard, a basic understanding of the colonization process is mandatory including the determination of the minimal bacterial load leading to a persistent colonization of broiler chickens. Therefore, we conducted a bivalent broiler colonization study close to real farming conditions without applying any antimicrobial selection pressure. ESBL- and AmpC- negative broiler chickens (Ross 308) were co- colonized on their third day of life with two strains: one CTX-M-15-producing Escherichia coli-ST410 and one CMY-2/mcr-1-positive E. coli-ST10. Colonization was assessed by cloacal swabs over the period of the trial, starting 24 h post inoculation. During the final necropsy, the contents of crop, jejunum, cecum, and colon were quantified for the occurrence of both bacterial strains. To define the minimal oral colonization dosage 104 to 101 colony forming units (cfu) were orally inoculated to four separately housed broiler groups (each n = 19, all animals inoculated) and a dosage of already 101 cfu E. coli led to a persistent colonization of all animals of the group after 3 days. To assure stable colonization, however, a dosage of 102 cfu E. coli was chosen for the subsequent seeder-bird trial. In the seeder-bird trial one fifth of the animals (seeder, n = 4) were orally inoculated and kept together with the non-inoculated animals (sentinel, n = 16) to mimic the route of natural infection. After 35 days of trial, all animals were colonized with both E. coli strains. Given the low colonization dosage and the low seeder/sentinel ratio, the rapid spread of ESBL- and AmpC- producing Enterobacteriaceae in conventional broiler farms currently seems inevitably resulting in an urgent need for the development of intervention strategies to reduce colonization of broilers during production.

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“…Previous studies with aerosol vaccination (ATTERBURY et al, 2010;DE CORT et al, 2015) showed that multiple sites including the digestive tract, respiratory tract, and conjunctiva are stimulated during aerosol vaccination, resulting in a systemic immune response, while oral administration of the vaccine stimulates a localized immune response in the intestinal tract. De Cort et al (2015) also suggested that oral immunization with live attenuated vaccine induces a mechanism that inhibits colonization and protects the bird from subsequent challenge, since young chickens are still immature and vaccination is not effective protection against Salmonella infection during the first days of life.…”

Section: Discussionmentioning

confidence: 99%

Salmonella Typhimurium Vaccine to Control a Brazilian Salmonella Heidelberg Strain in Broiler Chickens

Hayashi¹,

Tujimoto-Silva²,

Muñíz³

et al. 2018

Ars Vet

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Foodborne Salmonella infections in humans, which results from the consumption of contaminated poultry meat and eggs, are a major public health concern. Vaccination of animals against Salmonella is one strategy to prevent these infections and reduce the risks to public health. Live attenuated Salmonella enterica vaccines can confer protection against salmonellosis by inducing both cell-mediated and mucosal immune responses. This study assessed a live, attenuated Salmonella enterica Typhimurium (ST) vaccine in broiler chickens against a heterologous challenge with Salmonella Heidelberg (SH) by evaluating bacterial quantification, immune cells infiltration, and cytokine gene expression in the cecum. The treatments were: T1, non-vaccinated, non-challenged; T2, non-vaccinated, SH-challenged; T3, ST-vaccinated and SH-challenged. At 28 days of age, the ST-vaccinated group had significantly recovered reduction of SH in the crop (P<0,01) and cecum (P = 0,021) compared to the non-vaccinated SH-challenged group, with no significant changes (P˃0,05) in macrophages, T CD4+, or T CD8+ cells dynamics during the same period. Aerosol vaccination on the first day promoted greater interleukin-12 expression in the liver (P<0,05) and interleukin-10 expression and T CD8+ cells in the ileum 16 hours after housing. After prime-boosted oral immunization on the 13th day, the vaccinated group had greater expression of macrophages and T CD4+ cells in the liver (P<0,05) than the control group. Two doses of a live ST-attenuated vaccine promoted a partial cross-protective effect against SH strain UFPR1 challenge in broilers.

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Administration of a Salmonella Enteritidis ΔhilAssrAfliG strain by coarse spray to newly hatched broilers reduces colonization and shedding of a Salmonella Enteritidis challenge strain

Cited by 17 publications

References 19 publications

Immune protection of chickens conferred by a vaccine consisting of attenuated strains of Salmonella Enteritidis, Typhimurium and Infantis

Immune protection of chickens conferred by a vaccine consisting of attenuated strains of Salmonella Enteritidis, Typhimurium and Infantis

Low Dose Colonization of Broiler Chickens With ESBL-/AmpC- Producing Escherichia coli in a Seeder-Bird Model Independent of Antimicrobial Selection Pressure

Salmonella Typhimurium Vaccine to Control a Brazilian Salmonella Heidelberg Strain in Broiler Chickens

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