Administration of a live culture ofLactococcus lactisDPC 3147 into the bovine mammary gland stimulates the local host immune response, particularlyIL-1β andIL-8gene expression

Abstract

Mastitis is one of the most costly diseases to the dairy farming industry. Conventional antibiotic therapy is often unsatisfactory for successful treatment of mastitis and alternative treatments are continually under investigation. We have previously demonstrated, in two separate field trials, that a probiotic culture, Lactococcus lactis DPC 3147, was comparable to antibiotic therapy to treat bovine mastitis. To understand the mode of action of this therapeutic, we looked at the detailed immune response of the host to delivery of this live strain directly into the mammary gland of six healthy dairy cows. All animals elicited signs of udder inflammation 7 h post infusion. At this time, clots were visible in the milk of all animals in the investigation. The most pronounced increase in immune gene expression was observed in Interleukin (IL)-1b and IL-8, with highest expression corresponding to peaks in somatic cell count. Infusion with a live culture of a Lc. lactis leads to a rapid and considerable innate immune response.

Infusão intramamária de Weissella confusa afeta a contagem de células somáticas e a contagem diferencial de leucócitos no leite

“…Similar results to those found in this study were reported by BEECHER et al (2009), who evaluated in detail the immune response following the infusion of live cultures of Lactococcus lactis DPC 3147 into the mammary glands of cows. These authors noted that during the first 72 hours PI, quarters where L. lactis was applied showed higher levels of SCC than control quarters.…”

“…For this reason, new mastitis control methods continue to be studied. The use of live lactic acid bacterial cultures as therapeutic agents against bovine mastitis is currently a topic of research (BEECHER et al, 2009;.…”

“…In order to reduce the problem of mastitis in dairy cows, several strategies have been established for its treatment and prevention. These measures include antimicrobial therapy and studies of the potentials of molecules with bactericidal and/or bacteriostatic activities (BEECHER et al, 2009 increase in recent years. For this reason, new mastitis control methods continue to be studied.…”

Effects ofLactobacillus acidophilussupplementation for improvingin vitrorumen fermentation characteristics of cereal straws

“…Lactobacillus has been used widely as a feed additive in the dairy cattle industry to improve intestinal health, feed conversion efficiency and milk production (Chen et al 2013). Dietary supplementation of Lactobacillus was reported to increase milk production, reduce the count of somatic cells in milk (Jiang et al 2008) and reduce the probability of mastitis by positively regulating interleukin-1 (IL-1) and IL-8 in the mammary gland (Beecher et al 2009). However, those studies did not document the specific strain of L. acidophilus, and to our knowledge, there is no research evaluating the supplemental efficacy of L. acidophilus in in vitro fermentation experiments.…”

Inhibition of Staphylococcus aureus Invasion into Bovine Mammary Epithelial Cells by Contact with Live Lactobacillus casei

“…Such properties are harnessed in the development of vaginal probiotics used to prevent urogenital infections (18). Recently, the use of a bacteriocin-producing Lactococcus lactis strain was reported to be as efficient as a conventional antibiotic therapy to treat staphylococcal mastitis (19,20). Encouraging results were also obtained with a Lactobacillus perolens strain which was able to inhibit several mastitis-causing pathogens in vitro, to coaggregate with all of them, and to adhere to bovine teat canal epithelial cells without affecting udder aspect or the appearance of milk (21).…”