“…Studies with nonhuman primates or mice have also demonstrated that immunization with baculovirus-expressed recombinant proteins or DNA vaccines composed of different combinations of IMV-and EEVassociated proteins-such as A33R, B5R, and L1R (14,15), A27L, A33R, L1R, and B5R (21), or A27L, B5R, L1R, A33R, and D8L (41)-result in the induction of high levels of neutralizing antibodies and in the protection of mice from lethal challenge with vaccinia virus. In this study, we describe the expression of immunogenic recombinant A27L, D8L, and B5R vaccinia virus proteins by E. coli and demonstrate that subcutaneous immunization of BALB/c mice stimulated high levels of protein-specific and virus-cross-reactive immunoglobulin G (IgG) responses.…”