Adjuvant efficacy of G2 (buffalo spleen extraction) against Yersinia septicemia in rainbow trout (Onchorhynchus mykiss)

Shafie, S. S. El; Soltani, Elahe; Soltani, Mehdi; Hazrati, Saleh Mohaghegh

doi:10.1016/j.fsi.2018.08.011

Cited by 2 publications

(1 citation statement)

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“…Serum lysozyme levels were determined by turbidimetric method according to procedure described by Ellis () and Shafie, Soltani, Soltani, and Mohaghegh Hazrati () with slight modification. A 10 μl volume of serum sample was mixed with 200 μl Micrococcus luteus (0.2 μg/ml, Sigma‐Aldrich) suspension in 0.5 mM sodium phosphate buffer (pH 6.2) and optical density was measured after 1 and 6 min at 530 nm.…”

Section: Methodsmentioning

confidence: 99%

Growth performance, immune response and disease resistance of rainbow trout (Oncorhynchus mykiss) fedThymus vulgarisessential oils

et al. 2019

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Efficacy of Thymus vulgaris essential oils was assessed on growth, immune response and disease resistance of rainbow trout (Oncorhynchus mykiss). Fish weighing 10 g were fed with dietary supplemented of the oils at 0.5, 1.0 and 2.0 ml/kg feed for 2 months. Fish fed with the oils at 0.5 ml/kg feed demonstrated a better weight gain and specific growth rate, compared to other treatments (p < .05). Fish fed with 1.0 ml the oils showed the highest up‐regulation of complement component 3 (C3) and (cluster of differentiation 4) (CD4) genes expression (p < .05), while lysozyme gene expression level significantly increased in fish fed with 2.0 ml of the oils. In addition, at the end of the experiment, the expression of C3 and CD4 genes were significantly up‐regulated in fish fed with 1.0 and 2.0 ml of the oils, while IL‐1ß and lysozyme genes expression levels were significantly decreased in fish fed 2.0 ml oils, towards the end of the trial (p < .05). There was a fluctuation in the levels of Alanine aminotransferase, Aspartate aminotransferase and Alkaline phosphatase in all treatments during the experiment. When treated fish were challenged with Aeromonas hydrophila, the highest survival rate was observed in 0.5 ml/kg treatment, followed by 2 and 1 mg/kg treatments. Overall, these findings demonstrated that dietary administration of T. vulgaris oils especially at 0.5 mg/kg feed can be considered as a potential component for enhancing of the growth, immune responses and disease resistance of trout against motile Aeromonas septicemia caused by A. hydrophila.

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Section: Methodsmentioning

confidence: 99%