Adjuvant effect of zinc oxide on Th2 but not Th1 immune responses in mice

Matsumura, Misa; Nagata, Masafumi; Nakamura, Kazuichi; Kawai, Motoyuki; Baba, Tsuneo; Yamaki, Kouya; Yoshino, Shigefumi

doi:10.3109/08923970903124627

Cited by 30 publications

(25 citation statements)

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“…This suggests that oral tolerance induced by the mucosal immune system in the gut might be modulated by such very small size of particles. In addition, our recent studies revealed that treatment of mice with ultrafine ZnO was followed by the enhancement of immune responses, especially on T H 2 responses (Matsumura et al, 2010). The similar adjuvant effect was observed when mice were exposed to DEP (Yoshino and Sagai, 1999).…”

Section: Discussionsupporting

confidence: 49%

“…Yoshino and Sagai (1999) demonstrated that mice exposed to DEP had enhanced production of HELspecific IgG 2a and IgG 1 that was associated with increased secretion of IFNγ and IL-4, respectively, suggesting that DEP appears to have adjuvant activity on both T H 1 and T H 2 immune responses. On the other hand, Matsumura et al (2010) revealed that treatment of mice with OVA plus ZnO was followed by greater increases in anti-OVA IgG 1 /-IgE and IL-4/IL-5, but not in anti-OVA IgG 2a and IFNγ compared with that with OVA alone, indicating that ZnO seems to selectively stimulate T H 2 but not T H 1 responses. The blockade of induction of oral tolerance by DEP was associated with marked prevention of the suppression of both T H 1 and T H 2 secretion by oral antigen.…”

Section: Discussionmentioning

confidence: 95%

“…This suggests that oral tolerance might be modulated by such very small size of particles. Moreover, our recent studies showed that ultrafine ZnO had an ability to enhance immune responses in mice (Matsumura et al, 2010) …”

Section: Introductionmentioning

confidence: 99%

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Effect of ultrafine zinc oxide (ZnO) nanoparticles on induction of oral tolerance in mice

Matsumura¹,

Takasu²,

Nagata³

et al. 2010

Journal of Immunotoxicology

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Section: Discussionsupporting

confidence: 49%

Section: Discussionmentioning

confidence: 95%

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Effect of ultrafine zinc oxide (ZnO) nanoparticles on induction of oral tolerance in mice

Matsumura¹,

Takasu²,

Nagata³

et al. 2010

Journal of Immunotoxicology

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“…The assay to measure OVA-specific antibodies was performed as described previously (Matsumura et al 2010). IgG, IgG 1 , IgG 2a , and IgE antibodies specific for OVA were each measured by ELISA.…”

Section: Measurement Of Ova-specific Antibodiesmentioning

confidence: 99%

Amorphous nanosilica particles block induction of oral tolerance in mice

Toda

Yoshino

2016

Journal of Immunotoxicology

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The mucosal immune system is exposed to non-self antigens in food and the gut microbiota. Therefore, the recognition of orally ingested non-self antigens is suppressed in healthy individuals to avoid excessive immune responses in a process called ''oral tolerance''. The breakdown of oral tolerance has been cited as a possible cause of food allergy, and amorphous silica nanoparticles (nSP) have been implicated in this breakdown. As nSP are widely used in foodstuffs and other products, exposure to them is increasing; thus, investigations of any effects of nSP on oral tolerance are urgent. This study evaluated the effects of nSP30 (particle diameter ¼ 39 nm) on immunological unresponsiveness induced in mice with oral ovalbumin (OVA). Specifically, production of OVA-specific antibodies, splenocyte proliferation in response to OVA, and effects on T-helper (T H )-1, T H 2, and T H 17 responses (in terms of cytokine and IgG/IgE subclass expression) were evaluated. nSP30 increased the levels of OVA-specific IgG in OVA-tolerized mice and induced the proliferation of OVA-immunized splenocytes in response to OVA in a dose-related manner. nSP30 also increased the expression of OVA-specific IgG 1 , IgE, and IgG 2a , indicating stimulation of the T H 1 and T H 2 responses. The expression of interferon (IFN)-c (T H 1), interleukin (IL)-4 and IL-5 (T H 2), and IL-17 (T H 17) was also stimulated in a dose-related manner by nSP30 in splenocytes stimulated ex vivo with OVA. The induction of tolerance by OVA, the production of anti-OVA IgG antibodies, and proliferation of splenocytes in response to OVA was inhibited by nSP30 in conjunction with OVA and was dose-related. The nSP30 enhanced T H 1 and T H 2 responses that might prevent the induction of oral tolerance. Overall, this study showed that the abrogation of OVA-induced oral tolerance in mice by exposure to nSP30 was dose-related and that nSP30 stimulated T H 1, T H 2, and T H 17 responses. ARTICLE HISTORY

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“…Aggravation of asthma is unlikely to be caused by cytotoxicity of the NPs because exposure by the respiratory tract and by other routes (subcutaneous, intraperitoneal), where no direct contact with the alveolar epithelium occurred, caused the same effects. [113][114][115] The mechanisms for amplifying pre-existing pathologies have been proposed through the following mechanisms:…”

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confidence: 99%

Value of phagocyte function screening for immunotoxicity of nanoparticles in vivo

Frőhlich¹

2015

IJN

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Nanoparticles (NPs) present in the environment and in consumer products can cause immunotoxic effects. The immune system is very complex, and in vivo studies are the gold standard for evaluation. Due to the increased amount of NPs that are being developed, cellular screening assays to decrease the amount of NPs that have to be tested in vivo are highly needed. Effects on the unspecific immune system, such as effects on phagocytes, might be suitable for screening for immunotoxicity because these cells mediate unspecific and specific immune responses. They are present at epithelial barriers, in the blood, and in almost all organs. This review summarizes the effects of carbon, metal, and metal oxide NPs used in consumer and medical applications (gold, silver, titanium dioxide, silica dioxide, zinc oxide, and carbon nanotubes) and polystyrene NPs on the immune system. Effects in animal exposures through different routes are compared to the effects on isolated phagocytes. In addition, general problems in the testing of NPs, such as unknown exposure doses, as well as interference with assays are mentioned. NPs appear to induce a specific immunotoxic pattern consisting of the induction of inflammation in normal animals and aggravation of pathologies in disease models. The evaluation of particle action on several phagocyte functions in vitro may provide an indication on the potency of the particles to induce immunotoxicity in vivo. In combination with information on realistic exposure levels, in vitro studies on phagocytes may provide useful information on the health risks of NPs.

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Adjuvant effect of zinc oxide on Th2 but not Th1 immune responses in mice

Abstract: These results suggest that ZnO appears to have an adjuvant effect on the immune system, especially Th2 but not Th1 immune responses.

Cited by 30 publications

References 31 publications

Effect of ultrafine zinc oxide (ZnO) nanoparticles on induction of oral tolerance in mice

Effect of ultrafine zinc oxide (ZnO) nanoparticles on induction of oral tolerance in mice

Amorphous nanosilica particles block induction of oral tolerance in mice

Value of phagocyte function screening for immunotoxicity of nanoparticles in vivo

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Adjuvant effect of zinc oxide on Th2 but not Th1 immune responses in mice

Abstract: These results suggest that ZnO appears to have an adjuvant effect on the immune system, especially Th2 but not Th1 immune responses.

Cited by 30 publications

References 31 publications

Effect of ultrafine zinc oxide (ZnO) nanoparticles on induction of oral tolerance in mice

Effect of ultrafine zinc oxide (ZnO) nanoparticles on induction of oral tolerance in mice

Amorphous nanosilica particles block induction of oral tolerance in mice

Value of phagocyte function screening for&nbsp;immunotoxicity of nanoparticles in vivo

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Product

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Value of phagocyte function screening for immunotoxicity of nanoparticles in vivo