Adjuvant effect of mesoporous silica SBA-15 on anti-diphtheria and anti-tetanus humoral immune response

Trezena, Aryene Góes; Filho, Pedro Leonidas Oseliero; Silva, Luís Carlos Cides da; Oliveira, Cristiano L. P.; Lopes, José Luiz de Souza; Antonio, Nayara da Silva; Dettmann, Viktor Fonseca Barbosa; Akamatsu, Milena Apetito; Martins, Tereza da Silva; Ribeiro, Orlando; Fantini, Márcia Carvalho de Abreu; Sant’Anna, Osvaldo A.; Tino-De-Franco, Milene

doi:10.1016/j.biologicals.2022.10.001

Cited by 5 publications

(4 citation statements)

References 48 publications

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“… 51–56 In fact, by modeling the SAXS data, in combination with the NAI results, it was possible to conclude that a fraction of the Ac2-26 peptide was loaded into the SBA-15 mesopores. In contrast, the remaining amount is on the silica surface, a similar scenario observed in previous studies, 44 , 57 in which the authors loaded proteins instead of peptides. As suggested by Rasmussen et al, 58 the biological content of the SBA-15 macroporosity is still being protected against harsh environmental conditions such as the TGI.…”

Section: Discussionsupporting

confidence: 84%

Development of Ac2-26 Mesoporous Microparticle System as a Potential Therapeutic Agent for Inflammatory Bowel Diseases

Broering,

Oseliero Filho,

Borges

et al. 2024

IJN

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Inflammatory bowel diseases (IBDs) disrupt the intestinal epithelium, leading to severe chronic inflammation. Current therapies cause adverse effects and are expensive, invasive, and ineffective for most patients. Annexin A1 (AnxA1) is a pivotal endogenous anti-inflammatory and tissue repair protein in IBD. Nanostructured compounds loading AnxA1 or its active N-terminal mimetic peptides improve IBD symptomatology. Methods: To further explore their potential as a therapeutic candidate, the AnxA1 N-terminal mimetic peptide Ac2-26 was incorporated into SBA-15 ordered mesoporous silica and covered with EL30D-55 to deliver it by oral treatment into the inflamed gut. Results:The systems SBA-Ac2-26 developed measurements revealed self-assembled rod-shaped particles, likely on the external surface of SBA-15, and 88% of peptide incorporation. SBA-15 carried the peptide Ac2-26 into cultured Raw 264.7 macrophages and Caco-2 epithelial cells. Moreover, oral administration of Eudragit-SBA-15-Ac2-26 (200 μg; once a day; for 4 days) reduced colitis clinical symptoms, inflammation, and improved epithelium recovery in mice under dextran-sodium sulfate-induced colitis. Discussion: The absorption of SBA-15 in gut epithelial cells is typically low; however, the permeable inflamed barrier can enable microparticles to cross, being phagocyted by macrophages. These findings suggest that Ac2-26 is successfully delivered and binds to its receptors in both epithelial and immune cells, aligning with the clinical results. Conclusion: Our findings demonstrate a simple and cost-effective approach to delivering Ac2-26 orally into the inflamed gut, highlighting its potential as non-invasive IBD therapy.

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Section: Discussionsupporting

confidence: 84%

Development of Ac2-26 Mesoporous Microparticle System as a Potential Therapeutic Agent for Inflammatory Bowel Diseases

Broering,

Oseliero Filho,

Borges

et al. 2024

IJN

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“…The SBA-15+MC model was successfully applied to model SBA-15 with expanded mesopores, while the SBA-15 model, despite yielding satisfactory fits, failed to recover the correct size distribution and the values of the other fit parameters. In fact, the increase of mesopore width is followed by a decrease in the structural order (Garcia et al, 2016), but this is necessary to incorporate molecules of larger sizes, for instance diphtheria and tetanus anatoxins (Trezena et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Retrieving the size distribution of SBA-15 mesopores from small-angle X-ray scattering data using a Monte Carlo method

Tan,

Gerbelli,

Fantini

et al. 2023

J Appl Cryst

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A Monte Carlo (MC) method was introduced into a state-of-the-art model used to analyse small-angle X-ray scattering (SAXS) data of SBA-15, an ordered mesoporous material with many applications. With this new procedure, referred to herein as the SBA-15+MC model, it is possible to retrieve the size distribution of the mesopores, D(r), in a free modelling approach. To achieve this, two main points were addressed: (i) based on previous implementations, the method was adapted to work with long core–shell cylinders; (ii) since the MC model requires longer processing times, strategies to speed up the calculations were developed, which included a simplified version of the original model used to analyse SAXS data of SBA-15 (referred to as the SBA-15 model) as well as the determination of several structural features from the SAXS curve prior to the fit. The new model was validated with simulated data and later used to fit experimental SAXS curves of SBA-15. The obtained results show that the SBA-15 model only works well because the mesopore size distribution of SBA-15 is narrow, whereas the new approach can be successfully used in cases where D(r) is wider and/or has a more complex profile, such as SBA-15 with expanded mesopores. Even though a specific SAXS example was chosen to prove the model, the strategies presented herein are general and suitable for inclusion in other models aimed at the analysis of SBA-15 and similar ordered mesoporous materials.

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“…Furthermore, mesoporous SiO 2 with a pore size of 2–50 nm can encapsulate compounds, such as anticancer agents, or biomolecules, such as antigen proteins, within its regularly structured pores and release them in vivo. Because of these properties, various studies have been conducted on the feasibility of their use as drug delivery vehicles [ 6 , 7 , 8 ] and vaccine carriers [ 9 , 10 , 11 ]. For medical applications involving biological administration, it is important to evaluate SiO 2 microsphere aggregation and closely assess the uniform binding of compounds and biomolecules to individual microspheres.…”

Section: Introductionmentioning

confidence: 99%

Particle Size Measurement and Detection of Bound Proteins of Non-Porous/Mesoporous Silica Microspheres by Single-Particle Inductively Coupled Plasma Mass Spectrometry

Miyashita,

Ogura,

Matsuura

et al. 2024

Molecules

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Single-particle inductively coupled plasma mass spectrometry (spICP-MS) has been used for particle size measurement of diverse types of individual nanoparticles and micrometer-sized carbon-based particles such as microplastics. However, its applicability to the measurement of micrometer-sized non-carbon-based particles such as silica (SiO2) particles is unclear. In this study, the applicability of spICP-MS to particle size measurement of non-porous/mesoporous SiO2 microspheres with a nominal diameter of 5.0 µm or smaller was investigated. Particle sizes of these microspheres were measured using both spICP-MS based on a conventional calibration approach using an ion standard solution and scanning electron microscopy as a reference technique, and the results were compared. The particle size distributions obtained using both techniques were in agreement within analytical uncertainty. The applicability of this technique to the detection of metal-containing protein-binding mesoporous SiO2 microspheres was also investigated. Bound iron (Fe)-containing proteins (i.e., lactoferrin and transferrin) of mesoporous SiO2 microspheres were detected using Fe as a presence marker for the proteins. Thus, spICP-MS is applicable to the particle size measurement of large-sized and non-porous/mesoporous SiO2 microspheres. It has considerable potential for element-based detection and qualification of bound proteins of mesoporous SiO2 microspheres in a variety of applications.

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Adjuvant effect of mesoporous silica SBA-15 on anti-diphtheria and anti-tetanus humoral immune response

Cited by 5 publications

References 48 publications

Development of Ac2-26 Mesoporous Microparticle System as a Potential Therapeutic Agent for Inflammatory Bowel Diseases

Development of Ac2-26 Mesoporous Microparticle System as a Potential Therapeutic Agent for Inflammatory Bowel Diseases

Retrieving the size distribution of SBA-15 mesopores from small-angle X-ray scattering data using a Monte Carlo method

Particle Size Measurement and Detection of Bound Proteins of Non-Porous/Mesoporous Silica Microspheres by Single-Particle Inductively Coupled Plasma Mass Spectrometry

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