Adjuvant effect of an extract from Cochinchina momordica seeds on the immune responses to ovalbumin in mice

Xiao, Chen; Hu, Songhua; Rajput, Zahid Iqbal

doi:10.1007/s11703-007-0017-8

Cited by 21 publications

(16 citation statements)

References 13 publications

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“…3. Such a dosedependent relationship was also found previously when OVA was used as the antigen in a mouse model (31).…”

Section: Discussionsupporting

confidence: 84%

“…Our previous investigation has shown that an extract from a traditional Chinese medicine, ECMS, has an adjuvant effect on the immune response elicited by OVA in mice (31). In addition, ECMS has been found effective in stimulation of the immune response to vaccination against H5N1 avian influenza in chickens (24).…”

Section: Discussionmentioning

confidence: 99%

“…Oil emulsion provides the depot effect with progressive release of the antigens at the site of injection. ECMS has immunomodulatory capacity (31). The synergism between ECMS and oil may be due to the depot effect of oil emulsion in combination with the immunomodulatory effect of ECMS.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Enhancement of Serological Immune Responses to Foot-and-Mouth Disease Vaccine by a Supplement Made of Extract of Cochinchina Momordica Seeds

Xiao

Rajput

Liu

et al. 2007

Clin Vaccine Immunol

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Foot-and-mouth disease (FMD) is a highly contagious disease affecting cloven-hoofed animals. Vaccination against FMD is a routine practice in many countries where the disease is endemic. This study was designed first to investigate the extract of the seeds of Momordica cochinchinensis (Lour.) Spreng. (ECMS) for its adjuvant effect on vaccination of inactivated FMDV antigens in a guinea pig model and then to evaluate the supplement of ECMS in oil-emulsified FMD vaccines for its immunopotentiation in pigs. The results indicated that ECMS and oil emulsion act synergistically as adjuvants to promote the production of FMDV-and VP1-specific immunoglobulin G (IgG) and subclasses in guinea pigs. A supplement of ECMS in a commercial FMD vaccine significantly enhanced FMDV-specific indirect hemagglutination assay titers as well as VP1-specific IgG and subclasses in pigs. Therefore, ECMS could be an alternative approach to improving swine FMD vaccination when the vaccine is poor to induce an effective immune response.Foot-and-mouth disease (FMD) is a highly contagious disease affecting cloven-hoofed animals, particularly domestic species, such as cattle, swine, and sheep. Financial losses due to FMD are huge, and it is believed to be the most economically important animal disease in the world (26). Vaccination against FMD is a routine practice in many countries where the disease is endemic. Most commercially available FMD vaccines are inactivated whole-virus preparations containing oil emulsions as an adjuvant to improve their efficacy. However, some FMD vaccines have been reported to induce poor immune responses. For example, Xie et al. (33) observed that only 20.9% of piglets produced immune responses with antibody titers high enough for protection following vaccination against FMD (type O) in the Ningxia province of China. Hao et al. (12) analyzed 91 serum samples of pigs having received vaccination against FMD (type O) and found that only 31.9% of the samples had antibody titers required for immune protection immunity. Therefore, there is a need to improve currently available FMD vaccines in order to effectively protect animals from FMD infection.Our previous research has demonstrated that a supplement of saponin extracted from the bark of Quillaja saponaria Molina (Quil A) in vaccines significantly enhances immune responses to FMD vaccination (32). However, Quil A may be limited in veterinary use because of its hemolytic activity (23). Another study has shown that an extract from a traditional Chinese medicine, the seeds of Momordica cochinchinensis (Lour.) Spreng. (ECMS), has an adjuvant effect on the immune responses elicited by ovalbumin (OVA), with a much lower hemolytic activity (30). ECMS has also been proven effective in enhancing immune responses to vaccination against avian influenza (H5N1) in chickens (24). The medical use of the seeds has been described in ancient Chinese medical literature, Kai Bao Materia Medica, from the Song Dynasty (AD 793) (10) and is currently included in both Chinese pharmacopoei...

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“…3. Such a dosedependent relationship was also found previously when OVA was used as the antigen in a mouse model (31).…”

Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Enhancement of Serological Immune Responses to Foot-and-Mouth Disease Vaccine by a Supplement Made of Extract of Cochinchina Momordica Seeds

Xiao

Rajput

Liu

et al. 2007

Clin Vaccine Immunol

Self Cite

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“…A hemolytic assay was performed mainly as described by Xiao et al (30). Briefly, red blood cells (RBC) from a healthy New Zealand rabbit (Experimental Animal Center of Zhejiang Traditional Chinese Medicine College) were washed three times with physiological saline at 180 ϫ g for 10 min and adjusted to 0.5%.…”

Section: Methodsmentioning

confidence: 99%

Ginsenoside Rg1 and Aluminum Hydroxide Synergistically Promote Immune Responses to Ovalbumin in BALB/c Mice

Sun

Song

2008

Clin Vaccine Immunol

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The combined adjuvant effect of ginsenoside Rg1 and aluminum hydroxide (alum) on immune responses to ovalbumin (OVA) in mice was investigated. BALB/c mice were subcutaneously (s.c.) inoculated twice with OVA alone or in combination with Rg1, alum, or Rg1 plus alum. Samples were collected 2 weeks after the boosting for the measurement of anti-OVA immunoglobulin G (IgG) isotypes in sera and gamma interferon (IFN-␥) and interleukin-5 (IL-5) produced in singular splenocyte cultures. Delayed-type hypersensitivity (DTH) responses were measured in mice immunized as described above. After 10 days, the mice were injected s.c. with OVA at the footpads. Thereafter, the thickness of the footpads was measured once daily for 5 days. The results indicated that alum enhanced mainly Th2 (IgG1 and IL-5) responses (P < 0.05), while Rg1 enhanced both Th1 (IgG1 and IL-5) and Th2 (IgG2a, IFN-␥, and DTH) responses (P < 0.05). The highest immune responses were found in the mice injected with OVA solution containing both alum and Rg1. In addition, the hemolytic activity of Rg1 was much lower than that of Quil A. Therefore, Rg1 deserves further studies in order to tailor desired immune responses when a mixed Th1/Th2 immune response is needed.

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“…The ECMS was extracted following Xiao et al (2007a). Quil-A was supplied by Desert King Chile Ltd., Santiago, Chile.…”

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confidence: 99%

Short communication. Enhancement of the immune responses to vaccination against foot-and-mouth disease in mice by oral administration of Quillaja saponaria-A and extracts of Cochinchina momordica seed

Xiao

Rajput

Bao

et al. 2013

Span. j. agric. res.

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This study was designed to evaluate the effects of oral administration of extracts from Cochinchina momordica seed (ECMS) or Quillaja saponaria-A (Quil-A) on the immune responses in mice immunized with foot and mouth disease virus (FMDV)-serotype O vaccine. Forty-two imprinting control region (ICR) mice were randomly divided into seven groups of 6 animals in each group, and a dose of 400 µg of Quil-A or ECMS was orally administered for 1, 2 or 3 days. After that, the animals were subcutaneously immunized twice with FMD vaccine at 3-week intervals and blood samples were collected 2-weeks after boosting for measurement of FMDV-specific IgG and its subclasses. Spleens were collected for lymphocytes proliferation assay. Results indicated that serum FMDV-specific IgG and the IgG subclass responses were significantly enhanced in mice orally administered ECMS or Quil-A when compared with the control group (p < 0.05). Lymphocytes proliferation response to FMD vaccine was significantly enhanced by ECMS compared with the control (p < 0.05). This study illustrates that ECMS induced immunomodulatory effects and performed better than Quil-A.

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Adjuvant effect of an extract from Cochinchina momordica seeds on the immune responses to ovalbumin in mice

Cited by 21 publications

References 13 publications

Enhancement of Serological Immune Responses to Foot-and-Mouth Disease Vaccine by a Supplement Made of Extract of Cochinchina Momordica Seeds

Enhancement of Serological Immune Responses to Foot-and-Mouth Disease Vaccine by a Supplement Made of Extract of Cochinchina Momordica Seeds

Ginsenoside Rg1 and Aluminum Hydroxide Synergistically Promote Immune Responses to Ovalbumin in BALB/c Mice

Short communication. Enhancement of the immune responses to vaccination against foot-and-mouth disease in mice by oral administration of Quillaja saponaria-A and extracts of Cochinchina momordica seed

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