Adjuvant Approaches to Enhance Cryosurgery

Goel, Raghav; Anderson, Kyle; Slaton, Joel W.; Schmidlin, F.; Vercellotti, G. M.; Belcher, John D.; Bischof, John C.

doi:10.1115/1.3156804

Cited by 62 publications

(48 citation statements)

References 149 publications

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“…Finally, the maximum concentration to enhance the cryoprotective effect against red blood cells and islet cells using AFGPs is limited by the onset of increased cell damage due to specular ice crystal formation [118,143]. However, the negative effect of specular ice crystal growth was successfully used in cryosurgery using AFPs [175]. These results suggest that the cytotoxicity of AFGP8 is not only concentration-dependent but also cell specific.…”

Section: Discussionmentioning

confidence: 99%

Antifreeze Peptides and Glycopeptides, and Their Derivatives: Potential Uses in Biotechnology

et al. 2013

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Antifreeze proteins (AFPs) and glycoproteins (AFGPs), collectively called AF(G)Ps, constitute a diverse class of proteins found in various Arctic and Antarctic fish, as well as in amphibians, plants, and insects. These compounds possess the ability to inhibit the formation of ice and are therefore essential to the survival of many marine teleost fishes that routinely encounter sub-zero temperatures. Owing to this property, AF(G)Ps have potential applications in many areas such as storage of cells or tissues at low temperature, ice slurries for refrigeration systems, and food storage. In contrast to AFGPs, which are composed of repeated tripeptide units (Ala-Ala-Thr)n with minor sequence variations, AFPs possess very different primary, secondary, and tertiary structures. The isolation and purification of AFGPs is laborious, costly, and often results in mixtures, making characterization difficult. Recent structural investigations into the mechanism by which linear and cyclic AFGPs inhibit ice crystallization have led to significant progress toward the synthesis and assessment of several synthetic mimics of AFGPs. This review article will summarize synthetic AFGP mimics as well as current challenges in designing compounds capable of mimicking AFGPs. It will also cover our recent efforts in exploring whether peptoid mimics can serve as structural and functional mimics of native AFGPs.

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Section: Discussionmentioning

confidence: 99%

Antifreeze Peptides and Glycopeptides, and Their Derivatives: Potential Uses in Biotechnology

et al. 2013

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“…Most drug injection and CA strategies involve systemic or locoregional injection before or after CA. 11,23 They miss the potential ''driving'' role of an intervening ice ball on a locally coinjected fluid.…”

Section: Discussionmentioning

confidence: 99%

“…23 Various adjuvants such as chemicals, chemotherapeutics, or biologics have been used in preclinical and clinical applications as ''cryosensitizers'' before or after CA. 11 The goal was to bring the edge of the kill zone (cryolesion lethal margin at *À25 C) that cannot be imaged intraoperatively closer to the edge of the ice ball (cryolesion nonlethal margin *À0.5 C) that can be imaged intraoperatively. Such realization would allow an improved monitoring of the cryoablative zone, especially for large, irregular, and/ or ill-located tumors.…”

Section: Discussionmentioning

confidence: 99%

“…9,10 However, it is not known which timing, route of administration, formulation, or dosing for the associated cytotoxic drugs is best for improving the effectiveness and predictability of the cryoablative ice margin. [11][12][13] Many studies use drugs formulated with vehicles such as a matrix, capsule, or nanoparticle that facilitate tumor targeting, drug residence, slow release rate, and intracellular uptake. Promising combination therapy associated with nanoparticleencapsulated doxorubicin and CA 14 demonstrate enhanced eradication of cancer stem cells (CSTs) in vitro.…”

Section: Introductionmentioning

confidence: 99%

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Percutaneous Tumor Ablation

Xiao

et al. 2016

Technol Cancer Res Treat

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This acute study was aimed at exploring the ability of a cryoablative lesion to drive the distribution of a concomitant in situ injection of a free epirubicin-ethanol-ethiodol-methylene blue mixture. We report the feasibility and safety of this new percutaneous computed tomography-guided combinatorial ablative procedure on VX2 tumors. Eight New Zealand white rabbits bearing 16 tumors on both side of the back muscle were randomly selected and treated on the same day with the following procedures: (1) 8 concomitant cryoablation and interstitial chemotherapy and (2) 8 intratumor marginal chemotherapy. For the latter, an injection needle was positioned at the inner distal margin of a first selected tumor side, where the chemotherapy was delivered during 5 serial sequences. For the concomitant therapy, a single cryoneedle maintained the ice front at the tumor margin, where a needle delivered the drug dose during 5 freeze-injection-thaw sequences. Enhanced computed tomography scans on days 3, 7, and 10 assessed the tumor contours and the tracer localization. Two rabbits were killed on days 0, 3, 7, and 10 for gross and histopathological analyses. During the concomitant therapy, ioversol was distributed at the tumor and iceball margins along with the methylene blue. Enhanced computed tomography on days 3, 7, and 10 showed a focal enlarging defect of the tumor marginal enhancing rim. The rim coincided with focal necrosis at histopathology. During the intratumor chemotherapy procedure, computed tomography showed that the tracers distributed mostly over the tumor mass. No marginal necrosis was detected at histopathology. On day 10, the tumor size for the intratumor chemotherapy group was twice that of the concomitant therapy group. No adverse events were observed. In this VX2 tumor model, our image-guided concomitant therapy is feasible and may enhance the effectiveness of a free epirubicin tracer mixture at the tumor margin.

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“…The freezing technology performs increased control of the local disease in the area (i.e., reduced local recurrence of disease). And also can be used to enhance the natural destructive mechanisms of freezing within tissue [7].…”

Section: Introductionmentioning

confidence: 99%

Cryogenic frozen device for hepatocyte culture and responses

Chen

Liu

et al. 2012

2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)

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In clinical medicine, freezing treatment has been applied to patients for years. However, the combination of the micro/nano biochip (or Lab Chip) techniques and cryogenic frozen techniques were seldom proposed in the past. This paper reports a cryogenic frozen apparatus for the study of microfluidic liver tissue mimic hepatic cords responses on three dimension hepatocyte culture chip. We designed the micro cylinder about the height of 85 micrometer for loading hepatocyte to form three dimension tissue-mimic structures. The liver is organized into lobules which take the shape of polygonal prisms. Each lobule is typically hexagonal in cross section and is centered on the central vein. The bulk of the lobule consists of hepatocytes which are arranged into hepatic cords that are separated by sinusoid space. In liver function testing, the albumin secretion was affected 12% by freezing the hepatocyte ten minutes.

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Adjuvant Approaches to Enhance Cryosurgery

Cited by 62 publications

References 149 publications

Antifreeze Peptides and Glycopeptides, and Their Derivatives: Potential Uses in Biotechnology

Antifreeze Peptides and Glycopeptides, and Their Derivatives: Potential Uses in Biotechnology

Percutaneous Tumor Ablation

Cryogenic frozen device for hepatocyte culture and responses

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