Adjuvant activity of CpG-ODN formulated as a liquid crystal

Vallecillo, María F. Sánchez; Gamboa, Gabriela Verónica Ullio; Palma, Santiago Daniel; Harman, María F.; Chiodetti, Ana L.; Morón, Gabriel; Allemandi, Daniel Alberto; Pistoresi-Palencia, María C.; Maletto, Belkys A.

doi:10.1016/j.biomaterials.2013.12.002

Cited by 21 publications

(36 citation statements)

References 58 publications

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“…In the last years certain relationship of the presence of lipid domains with cellular function has been widely discussed [46]. In this context it is not difficult to relate the possible formation of condensed domains enriched in ASC 16 in cell membranes and the inflammatory effect observed in tissues exposed to large amount of this drug [8], or the ability of ASC 12 to mix in a single phase with phospholipids with a mild tissue irritation when used for drug permeation enhancer [5]. Taking into account the different pharmacological potential of each ASC n compound, we hypothesize that this may be strongly related to their surface properties and their ability to interact with lipid membranes.…”

Section: Discussionmentioning

confidence: 99%

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Alkyl esters of l-ascorbic acid: Stability, surface behaviour and interaction with phospholipid monolayers

Mottola

Vico

Villanueva

et al. 2015

Journal of Colloid and Interface Science

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Section: Discussionmentioning

confidence: 99%

“…On the other hand, ASC 16 has been proposed as the key component of a new and potent adjuvant preparation. The high immunization levels obtained were related to the induction of a local inflammatory response [8].…”

Section: Introductionmentioning

confidence: 99%

Alkyl esters of l-ascorbic acid: Stability, surface behaviour and interaction with phospholipid monolayers

Mottola

Vico

Villanueva

et al. 2015

Journal of Colloid and Interface Science

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“…This, together with an aqueous organization as large and highly compacted structures (Figure 1 and [11]), may account for its good adjuvant capacity recently reported for ASC16 coagels [8]. In addition, the effect of these coagels, when used as adjuvants, suggests a local inflammatory response induced by ASC16 as a main feature responsible for the obtained immunization.…”

Section: Discussionmentioning

confidence: 92%

“…The ASCn, used in different pharmacological preparations due to its amphiphilic nature and anti-oxidative power, have shown subtly differences on therapeutic effectiveness [7][8][9]27]. …”

Section: Discussionmentioning

confidence: 99%

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The amphiphilic alkyl ester derivatives of l -ascorbic acid induce reorganization of phospholipid vesicles

Giudice

Ambroggio

Mottola

et al. 2016

Biochimica et Biophysica Acta (BBA) - Biomembranes

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l-ascorbic acid alkyl esters (ASCn) are lipophilic forms of vitamin C, which maintain some of its antioxidant power. Those properties make this drug family attractive to be used in pharmacological preparations protecting other redox-sensible drugs or designed to reduce possible toxic oxidative processes. In this work, we tested the ability of l-ascorbic acid alkyl esters (ASCn) to modulate the structure, permeability, and rheological properties of phospholipid bilayers. The ASCn studied here (ASC16, ASC14, and ASC12) alter the structural integrity as well as the rheological properties of phospholipid membranes without showing any evident detergent activity. ASC14 appeared as the most efficient drug in destabilize the membrane structure of nano- and micro-size phospholipid liposomes inducing vesicle content leakage and shape elongation on giant unilamellar vesicles. It also was the most potent enhancer of membrane microviscosity and surface water structuring. Only ASC16 induced the formation of drug-enriched condensed domains after its incorporation into the lipid bilayer, while ASC12 appeared as the less membrane-disturbing compound, likely because of its poor, and more superficial, partition into the membrane. We also found that incorporation of ASCn into the lipid bilayers enhanced the reduction of membrane components, compared with soluble vitamin C. Our study shows that ASCn compounds, which vary in the length of the acyl chain, show different effects on phospholipid vesicles used as biomembrane models. Those variances may account for subtly differences in the effectiveness on their pharmacological applications.

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Endotoxin contamination in ovalbumin as viewed from a nano‐immunotherapy perspective

Pang

Liu

Wang

et al. 2021

WIREs Nanomed Nanobiotechnol

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Ovalbumin (OVA) is a model antigen commonly incorporated in smartly designed nanoparticles for delivery into antigen-presenting cells (APC), aiming to investigate the immune activity and therapeutic efficacy of nanoparticles that contain immunoregulatory compounds. However, the immunoresponse observed in nano-immunotherapy may unexpectedly arise from endotoxin impurity of OVA in the nanoparticles. Literature review shows that most researchers did not notice the importance of endotoxin-free OVA when used in nano-immunotherapy studies. Concentration at as low as 5 μg/ml OVA from Sigma-Aldrich (contains 0.625 ng/ml endotoxin) was able to activate APC such as dendritic cells and macrophages. Here, we proposed that the endotoxin impurity in OVA or the finished nanoproducts should be determined by both Limulus Amebocyte Lysate (LAL) and cell-based assay, to ensure the endotoxin-free quality of the nanoparticles. The endotoxin in OVA can be removed by endotoxin removal column and phase separation methods and endotoxin-free OVA can be purchased. This perspective alerts the researchers of endotoxin impurity of OVA that may transfer into the finished nanoparticles and introduce an unfavorable immunoregulatory function with false-positive results. OVA with minimal endotoxin level should be used in nano-immunotherapy studies to accurately reflect the true effects of nanoparticles on the immune system.

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Adjuvant activity of CpG-ODN formulated as a liquid crystal

Cited by 21 publications

References 58 publications

Alkyl esters of l-ascorbic acid: Stability, surface behaviour and interaction with phospholipid monolayers

Alkyl esters of l-ascorbic acid: Stability, surface behaviour and interaction with phospholipid monolayers

The amphiphilic alkyl ester derivatives of l -ascorbic acid induce reorganization of phospholipid vesicles

Endotoxin contamination in ovalbumin as viewed from a nano‐immunotherapy perspective

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