Adjuvant-active fraction from Albizia julibrissin saponins improves immune responses by inducing cytokine and chemokine at the site of injection

Sun, Hongxiang; He, Shuwang; Shi, Minghua

doi:10.1016/j.intimp.2014.07.021

Cited by 29 publications

(24 citation statements)

References 63 publications

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“…Saponins display the capability to reinforce cellular immunity and humoral immunity as well as relieve pain and enhance blood flow (Cai et al, ; Sun, Chen, Wang, Wang, & Zhou, ; Sun, He, & Shi, ). In this work, the concentration of haemoglobin was remarkably decreased in response to PRRSV infection, which is consistent with previous findings (Tao et al, ).…”

Section: Discussionmentioning

confidence: 99%

Therapeutic effects of saponin components on porcine reproductive and respiratory syndrome virus‐infected piglets

Zhang

Wang

et al. 2020

Animal Physiology Nutrition

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The present study aimed to evaluate the potential therapeutic effects of Anemoside B4 (AB4), Panax notoginseng saponins (PNS), Notoginsenoside R1 (SR1), Saikosaponin A (SSA) and Saikosaponin D (SSD) on piglets infected with porcine reproductive and respiratory syndrome virus (PRRSV). A total of 132 completely healthy piglets were randomly divided into 22 groups consisting of six animals each. Control piglets were intramuscularly injected with 2 ml of PRRSV (NJGC strain) solution containing 106 TCID50 virus/ml. For low‐, middle‐ and high‐dose saponin treatment groups, the piglets were initially administrated with the same volume of PRRSV solution, followed by intraperitoneal injection with AB4, PNS, SR1, SSA or SSD at 1, 5 or 10 mg/kg b.w. on day 3. The piglets in drug control group were intraperitoneally injected with 10 mg/kg b.w. of each saponin without prior PRRS challenge, while those in blank control group were injected with the same amount of normal saline. The results indicated that all the five saponin components could decrease the incidence and severity of PRRSV‐induced immunopathological damages, including the elevated body temperature, weight loss, anaemia and internal inflammation. Moreover, the saponin components could enhance protein absorption and immune responses. Taken together, this study reveals that the saponin components are effective against PRRSV infection and strengthen the immune system and thus may serve as potential antiviral therapeutic agents.

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Section: Discussionmentioning

confidence: 99%

Therapeutic effects of saponin components on porcine reproductive and respiratory syndrome virus‐infected piglets

Zhang

Wang

et al. 2020

Animal Physiology Nutrition

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“…[2,3]. Its chemical constituents include triterpenoid saponins, lignanoids, flavonoids, phenoloc glycosides, and other compounds [4]. The triterpenoid saponins have been proved to be the main bioactive principles of this crude drug [5].…”

Section: Introductionmentioning

confidence: 99%

“…Modern pharmacological studies show that Cortex Albiziae has a variety of activities including anticancer, anti‐angiogenic, antioxidant, anti‐fertility, anxiolytic activities . Its chemical constituents include triterpenoid saponins, lignanoids, flavonoids, phenoloc glycosides, and other compounds . The triterpenoid saponins have been proved to be the main bioactive principles of this crude drug .…”

Section: Introductionmentioning

confidence: 99%

“…In our previous study, the total saponin from the dry stem bark of A. julibrissin and its crude active fractions have been reported to possess the ability to improve antigen‐specific immune responses to ovalbumin and H5 avian influenza live vaccine . Furthermore, the purified active fraction of A. julibrissin saponin (AJSAF) was proved to be a promising adjuvant candidate for vaccine .…”

Section: Introductionmentioning

confidence: 99%

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Rapid annotation and structural characterization of saponins in the active fraction of Albizia julibrissin by high‐performance liquid chromatography coupled with quadrupole time of flight mass spectrometry based on accurate mass database

Liu

et al. 2019

J of Separation Science

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The purified active fraction of Albizia julibrissin saponin was proved to be a promising adjuvant candidate for vaccine. In this study, a simple, convenient, and practical strategy was established for characterizing the saponins in this purified active fraction. The personal accurate mass database including chemical structure, molecular formula, and theoretical mass was first constructed by collecting 110 reported known saponins from genus Albizia species. The raw data was obtained by high‐performance liquid chromatography coupled with quadrupole time of flight mass spectrometry. The potential compounds were extracted from raw data, and matched with the accurate mass databases. A series of saponin compounds were predicted and their chemical structures were characterized by interpreting the tandem mass spectrometry data. A total of 29 saponins including 10 new compounds and 5 first found saponins from A. julibrissin were successfully characterized in this purified active fraction using this new strategy.

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“…(AJSAF) would be a promising adjuvant candidate for vaccines. It has been proved to improve antigen-specific cellular and humoral immune responses, and simultaneously elicit mixed Th1/Th2 responses in mice to the H5 avian influenza vaccine [14] and porcine reproductive and respiratory syndrome virus vaccine [15]. In our previous studies, it was found that the colocalization of AJSAF with antigen or not significantly affected its adjuvant activity in mice.…”

Section: Introductionmentioning

confidence: 99%

Mechanisms of Mixed Th1/Th2 Responses in Mice Induced by Albizia julibrissin Saponin Active Fraction by in Silico Analysis

Jin

Wang

et al. 2020

Vaccines

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The purified active fraction of Albizia julibrissin saponin (AJSAF) is an ideal adjuvant candidate that improves antigen-specific both cellular and humoral immune responses and elicits mixed Th1/Th2 responses, but its mechanisms remain unclear. The key features of action of AJSAF were investigated in mice immunized with Newcastle disease virus-based recombinant influenza vaccine (rL-H5) and AJSAF at the same leg (AJSAF+rL-H5) or different legs (AJSAF/rL-H5). The adjuvant activity of AJSAF on rL-H5 is strictly dependent on their spatial colocalization. Serum H5 antigen (H5Ag)-specific IgG, IgG1, IgG2a, and IgG2b antibody titers in AJSAF+rL-H5 group were significantly higher than those in AJSAF/rL-H5 group. The mechanisms of selectivity of Th1 or Th2 in mice induced by AJSAF was explored by the transcriptomic and proteomic profiles of H5Ag-stimulated splenocytes from the immunized mice using gene microarray and two-dimensional difference gel electrophoresis coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Compared to rL-H5 alone, AJSAF/rL-H5 induced more differentially expressed genes (DEGs) than AJSAF+rL-H5, whereas AJSAF+rL-H5 upregulated higher mRNA expression of Th1 (T-bet, IFN-γ, TNF-α, IL-12β, and IL-12Rβ1) and Th2 (IL-10 and AICDA) immune response genes. The neutrophil response and its derived S100A8 and S100A9 might be involved in the AJSAF-mediated Th1 response. Meanwhile, AJSAF might induce the adaptive immune responses by improving a local innate immune microenvironment. These findings expanded the current knowledge on the mechanisms of action of saponin-based adjuvants, and provided new insights into how adjuvants shape adaptive immune responses.

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Adjuvant-active fraction from Albizia julibrissin saponins improves immune responses by inducing cytokine and chemokine at the site of injection

Cited by 29 publications

References 63 publications

Therapeutic effects of saponin components on porcine reproductive and respiratory syndrome virus‐infected piglets

Therapeutic effects of saponin components on porcine reproductive and respiratory syndrome virus‐infected piglets

Rapid annotation and structural characterization of saponins in the active fraction of Albizia julibrissin by high‐performance liquid chromatography coupled with quadrupole time of flight mass spectrometry based on accurate mass database

Mechanisms of Mixed Th1/Th2 Responses in Mice Induced by Albizia julibrissin Saponin Active Fraction by in Silico Analysis

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