Adjustments of the enzymatic complement for polyol biosynthesis and accumulation in diapausing cold-acclimated adults of Pyrrhocoris apterus

Košťál, Vladimı́r; Tollarová, Michaela; Šula, J

doi:10.1016/j.jinsphys.2004.01.006

Cited by 33 publications

(20 citation statements)

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“…The increase of pyruvate is not surprising as there must be an inhibitory block of glycolysis at this specific locus to favor polyols synthesis rather than TCA cycle [40]. Indeed, production of polyols requires a regulatory control of glycolysis and pentose cycle in order to divert the carbon flow from the main stream [40], [46]. In the present study, we found an up-regulation of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in D mummies (spot 1666).…”

Section: Discussionsupporting

confidence: 50%

Metabolic and Proteomic Profiling of Diapause in the Aphid Parasitoid Praon volucre

et al. 2012

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BackgroundDiapause, a condition of developmental arrest and metabolic depression exhibited by a wide range of animals is accompanied by complex physiological and biochemical changes that generally enhance environmental stress tolerance and synchronize reproduction. Even though some aspects of diapause have been well characterized, very little is known about the full range of molecular and biochemical modifications underlying diapause in non-model organisms.Methodology/Principal FindingsIn this study we focused on the parasitic wasp, Praon volucre that exhibits a pupal diapause in response to environmental signals. System-wide metabolic changes occurring during diapause were investigated using GC-MS metabolic fingerprinting. Moreover, proteomic changes were studied in diapausing versus non-diapausing phenotypes using a combination of two-dimensional differential gel electrophoresis (2D-DIGE) and mass spectrometry. We found a reduction of Krebs cycle intermediates which most likely resulted from the metabolic depression. Glycolysis was galvanized, probably to favor polyols biosynthesis. Diapausing parasitoids accumulated high levels of cryoprotective polyols, especially sorbitol. A large set of proteins were modulated during diapause and these were involved in various functions such as remodeling of cytoskeleton and cuticle, stress tolerance, protein turnover, lipid metabolism and various metabolic enzymes.Conclusions/SignificanceThe results presented here provide some first clues about the molecular and biochemical events that characterize the diapause syndrome in aphid parasitoids. These data are useful for probing potential commonality of parasitoids diapause with other taxa and they will help creating a general understanding of diapause underpinnings and a background for future interpretations.

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Section: Discussionsupporting

confidence: 50%

Metabolic and Proteomic Profiling of Diapause in the Aphid Parasitoid Praon volucre

et al. 2012

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“…The synthesis of these compounds is promoted by diapause [20]–[22], and is triggered by exposure to low temperatures [23]–[25]. At the early stages of insect diapause, a decline in the expression of enzymes involved in energetic metabolism has been observed [8], together with increased amounts of key enzymes involved in polyols production, such as glucose-6-P dehydrogenase, aldose reductase, and ketose reductase [26]. Then, during diapause development, the amount of accumulated polyols is modulated [26], with subsequent declines in temperature expected to have further effect on the synthesis of these compounds.…”

Section: Introductionmentioning

confidence: 99%

Deciphering the Metabolic Changes Associated with Diapause Syndrome and Cold Acclimation in the Two-Spotted Spider Mite Tetranychus urticae

et al. 2013

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Diapause is a common feature in several arthropod species that are subject to unfavorable growing seasons. The range of environmental cues that trigger the onset and termination of diapause, in addition to associated hormonal, biochemical, and molecular changes, have been studied extensively in recent years; however, such information is only available for a few insect species. Diapause and cold hardening usually occur together in overwintering arthropods, and can be characterized by recording changes to the wealth of molecules present in the tissue, hemolymph, or whole body of organisms. Recent technological advances, such as high throughput screening and quantification of metabolites via chromatographic analyses, are able to identify such molecules. In the present work, we examined the survival ability of diapausing and non-diapausing females of the two-spotted spider mite, Tetranychus urticae, in the presence (0 or 5°C) or absence of cold acclimation. Furthermore, we examined the metabolic fingerprints of these specimens via gas chromatography-mass spectrophotometry (GC-MS). Partial Least Square Discriminant Analysis (PLS-DA) of metabolites revealed that major metabolic variations were related to diapause, indicating in a clear cut-off between diapausing and non-diapausing females, regardless of acclimation state. Signs of metabolic depression were evident in diapausing females, with most amino acids and TCA cycle intermediates being significantly reduced. Out of the 40 accurately quantified metabolites, seven metabolites remained elevated or were accumulated in diapausing mites, i.e. cadaverine, gluconolactone, glucose, inositol, maltose, mannitol and sorbitol. The capacity to accumulate winter polyols during cold-acclimation was restricted to diapausing females. We conclude that the induction of increased cold hardiness in this species is associated with the diapause syndrome, rather than being a direct effect of low temperature. Our results provide novel information about biochemical events related to the cold hardening process in the two-spotted spider mite.

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“…Indeed, PPP is a major source for reductant (i.e. NADPH) and osmoprotectant production which are both used to counteract the damages caused by desiccation in insects [46–48]. We hypothesise that increased levels of both G6P and F6P at ODS depict an increase in activity of the PPP at ODS in female mosquitoes.…”

Section: Discussionmentioning

confidence: 99%

Comparative physiological plasticity to desiccation in distinct populations of the malarial mosquito Anopheles coluzzii

et al. 2016

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BackgroundIn West Africa, populations of the malaria vector mosquito, Anopheles coluzzii, are seasonally exposed to strong desiccating conditions during the dry season. Their dynamics strictly follows the pace of the availability of suitable larval development sites (water collections). Accordingly, mosquitoes can reproduce all year long where permanent breeding is possible, or stop reproduction and virtually disappear at the onset of the dry season when surface water dries up, like observed in temporary habitats of dry savannah areas. This highlights the strong adaptive abilities of this mosquito species, which relies at least in part, upon physiological and molecular mechanisms of specific signatures.MethodsHere, we analysed a range of physiological and molecular responses expressed by geographically different populations of An. coluzzii inhabiting permanent and temporary breeding sites from the north and the south-west of Burkina Faso. Four mosquito colonies, namely (i) Oursi, built from females breeding in permanent habitats of the north; (ii) Déou, from temporary northern habitats; (iii) Soumousso from south-western temporary breeding sites; and (iv) Bama, from permanent habitats of the same south-western zone, were reared in climatic chambers under contrasted environmental conditions, mimicking temperature, relative humidity and light regimen occurring in northern Burkina Faso. Female mosquitoes were analysed for the seasonal variation in their amounts of proteins, triglycerides and free-circulating metabolites. The expression level of genes coding for the adipokinetic (AKH-I) and the AKH/corazonin-related peptides (ACP) were also assessed and compared among populations and environmental conditions.ResultsOur analysis did not reveal an apparent pattern of physiological and molecular variations strictly correlated with either the larval ecotype or the geographical origin of the mosquitoes. However, specific distinct responses were observed among populations, suggesting that dry season survival may rely on more complex ecological parameters at a micro-habitat scale. Interestingly, the physiological and molecular data support the hypothesis that different aestivation abilities exist among populations of An. coluzzii inhabiting contrasted ecological settings. In particular, the striking metabotypes differentiation and the AKH mRNA expression level observed in females from temporary northern populations may suggest the existence of a “strong” aestivation strategy in these specimens.ConclusionOur work provides insights into the physiological and molecular basis of dry and rainy season responses in An. coluzzii, and highlights the important diversity of the mechanisms involved. Such results represent key data for understanding the ecophysiological mechanisms underpinning the strong adaptive potential of this malaria vector species, which undoubtedly contributes to the spreading of mosquito distribution areas in space and time.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-0...

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Adjustments of the enzymatic complement for polyol biosynthesis and accumulation in diapausing cold-acclimated adults of Pyrrhocoris apterus

Cited by 33 publications

References 41 publications

Metabolic and Proteomic Profiling of Diapause in the Aphid Parasitoid Praon volucre

Metabolic and Proteomic Profiling of Diapause in the Aphid Parasitoid Praon volucre

Deciphering the Metabolic Changes Associated with Diapause Syndrome and Cold Acclimation in the Two-Spotted Spider Mite Tetranychus urticae

Comparative physiological plasticity to desiccation in distinct populations of the malarial mosquito Anopheles coluzzii

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