Adjustment of the methodology of the tetrazolium test for estimating viability of Eugenia uniflora L. seeds during storage

Kaiser, Daiana Karoline; Freitas, Laura Cristiane Nascimento de; Biron, Rubia Priscila; Simonato, Simone Cristina; Bortolini, Michele Fernanda

doi:10.1590/2317-1545v36n31022

Cited by 10 publications

(13 citation statements)

References 25 publications

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“…Morong. ); Kaiser et al (2014) with pitanga (Brazilian cherry) (Eugenia uniflora L.); Gimenez et al (2014) with graviola (Annona cherimola Mill. ); Garlet et al (2015) with gold medallion tree (Cassia leptophylla Vogel.…”

Section: Introductionmentioning

confidence: 99%

Viability of Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea) seeds by tetrazolium test

et al. 2017

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Rapid tests have been essential to evaluate the physiological potential of seeds and the tetrazolium test is one of those which have been used by seed companies. The objective of the study was to establish the procedure for the tetrazolium test in Libidibia ferrea (Mart. Ex Tul.) L.P. Queiroz var. ferrea seeds. For this, three tetrazolium solution concentrations (0.05, 0.075 and 0.1%) and three staining periods (1, 3 and 6 hours) at temperatures of 35 to 40 °C were tested. The seeds were also evaluated by the germination test, whose result was compared with viability by the tetrazolium test. The experimental design was completely randomized in a factorial design 3 x 3 + 1 (three concentrations x three periods + a control = germination test) for each temperature. Viable seeds in the tetrazolium test were compared by Tukey’s test (p ≤ 0.05), while the comparison between the viable seeds with germination test was carried out by Dunnett’s test (p ≤ 0.05). It is recommended that the tetrazolium test for L. ferrea is carried out at a concentration of 0.05% for three-hour staining under 35 °C or 40 °C.

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Section: Introductionmentioning

confidence: 99%

Viability of Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea) seeds by tetrazolium test

et al. 2017

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“…em câmara fria (5±2 °C e 90% de umidade relativa do ar) e câmara seca (15±2 °C e 60% de umidade relativa do ar), Andrade et al (2000) verificaram que, após 30 dias de armazenamento, a porcentagem inicial de emergência (67%) reduziu 16 e 91%, respectivamente. Kaiser et al (2014) observaram que sementes de Eugenia uniflora L. armazenadas por 30 dias em geladeira (10 °C) apresentaram 94% de germinação. Benefícios potenciais do armazenamento em ambiente refrigerado também foi observado para sementes de E. calycina (VON BÜLOW et al, 1994), E. involucrata DC.…”

Section: Resultsunclassified

QUALIDADE DE SEMENTES DE Eugenia dysenterica DC. DURANTE O ARMAZENAMENTO

Silva

Vale

Silva

et al. 2019

Reveng

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Palavras-chave:Conservação emergência recalcitrante RESUMO A adoção de estratégias adequadas para o armazenamento de sementes é essencial para conservação da sua qualidade, ainda mais para aquelas classificadas como recalcitrantes.Objetivou-se, com este trabalho, avaliar a qualidade de sementes de Eugenia dysenterica DC. durante o armazenamento em ambiente natural e refrigerado. Os frutos foram coletados diretamente no chão e despolpados manualmente, e as sementes lavadas em água corrente, selecionadas quanto ao tamanho e armazenadas por 0; 7; 14; 21 e 28 dias em ambiente natural de laboratório (26,6 °C e 52,2% URA) e em refrigerador (8,4 °C e 49,2% URA). Para cada ambiente e período de armazenamento, foram realizadas as seguintes análises: emergência; índice de velocidade; tempo médio e primeira contagem de emergência de plântulas; comprimento da parte aérea e da raiz principal; massa fresca e seca da parte aérea e das raízes; massa seca total das plântulas; índice de qualidade de Dickson e teor de água. Independe do ambiente estudado, a porcentagem de emergência de plântulas se manteve acima de 84%, mesmo após 28 dias de armazenamento. O armazenamento das sementes de E. dysenterica DC. reduz a velocidade de emergência e o desempenho das plântulas, principalmente para aquelas armazenadas em ambiente natural. ABSTRACTThe adoption of adequate strategies for the storage of seeds is essential for their quality's conservation, even more for those classified as recalcitrant. The aim of this work was to evaluate the quality of Eugenia dysenterica DC.'s seeds during storage in a natural and refrigerated environment. The fruits were collected directly on the ground and manually pulped. The seeds were washed in running water, selected by size and stored for 0, 7, 14, 21 and 28 days in a natural laboratory's environment (26.6 °C and 52 °C, 2% URA) and in a refrigerator (8.4 °C and 49.2% URA). The following analyzes were performed for each environment and storage period: emergency, speed index, average time, first emergence's count of seedlings, length of the aerial part and the main root, fresh and dry mass of the aerial part and the roots, total dry mass of seedlings, quality index of Dickson and water content. The percentage of emergence's seedlings was above 84%, even after 28 days of storage, regardless the studied environment. The storage of E. dysenterica DC.'s seeds reduces the emergency speed and the seedlings' performance, mainly for those stored in a natural environment.

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“…If this moisture is reduced below a critical level during storage, the seeds will die (KING; ROBERTS, 1979). Kaiser et al (2014) reported that the high moisture content of Brazilian Cherry seeds (40.6 -43.4%) is characteristic of recalcitrant species, especially Eugenia genus, which moisture content varies from 40 to 70% at the moment of dispersion.…”

Section: Sexual Propagationmentioning

confidence: 99%

Advances in the propagation of Brazilian Cherry tree

et al. 2019

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Abstratct Brazilian Cherry tree is a native fruit tree belonging to the Myrtaceae family, with ample adaptation to the different edaphoclimatic conditions of Brazil, which makes this fruit widely known. However, there are still few commercial orchards, which may soon be reversed, since studies have shown the benefits of the consumption of this fruit for human health, as well as the potentialities for industrial processing. Thus, Brazilian Cherry tree will no longer be a backyard plant and will be grown in commercial orchards, which demands the production of seedlings with genetic quality. Sexual propagation with the use of seeds should be replaced by asexual propagation by means of cutting, grafting, spreading and tissue culture. The grafting method has proven to be efficient in more than 70% of cases and herbaceous cuttings have rooting greater than 77%. New studies on micropropagation and better control of herbaceous cutting processes should stimulate and economically increase the commercial production of this fruit for fresh consumption or processing. This review article included the results of other studies, addressing advances, limitations and protocols for propagation of Brazilian Cherry tree.

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Adjustment of the methodology of the tetrazolium test for estimating viability of Eugenia uniflora L. seeds during storage

Cited by 10 publications

References 25 publications

Viability of Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea) seeds by tetrazolium test

Viability of Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz var. ferrea) seeds by tetrazolium test

QUALIDADE DE SEMENTES DE Eugenia dysenterica DC. DURANTE O ARMAZENAMENTO

Advances in the propagation of Brazilian Cherry tree

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