Adjustment of common carp sperm concentration prior to cryopreservation: Does it matter?

Pataki, Bernadett; Horváth, Ákos; Mészáros, Gergely; Kitanović, Nevena; Ács, András; Hegyi, Árpád; Molnár, József; Csorbai, Balázs; Urbányi, Béla

doi:10.1016/j.aqrep.2022.101109

Cited by 2 publications

(2 citation statements)

References 23 publications

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“…First, as suggested by Dokina et al (2019), a hypotonic carp sperm cryoprotective medium has been successively applied for carp sperm using a low dilution rate (1:1). Second, several studies have demonstrated that application of low sperm dilution rates is not a critically dangerous factor for successful common carp sperm cryopreservation (Pataki et al, 2022;Sotnikov et al, 2023). These findings provide a robust background for the current study.…”

Section: Discussionmentioning

confidence: 55%

Optimizing aquaculture-scale common carp artificial reproduction: a novel approach to sperm cryopreservation using large-volume containers and elevated thawing temperatures

Sotnikov,

Rodina,

Gela

et al. 2024

Front. Mar. Sci.

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IntroductionThe successful cryopreservation of common carp sperm is crucial for its application in aquaculture and selective breeding programs. This study investigates the efficacy of cryopreserving sperm in large containers (5 mL) with a low dilution rate (1:1) in three different cryoprotective media and thawing in different conditions.MethodsThe developed method utilizes a low-ionic (hypotonic) cryoprotective medium, freezing with a controlled cooling rate, and high-temperature sperm thawing (60°C). The investigation employs a detailed spermatozoon motility assessment.ResultsPost-thaw motility of 32.3% ± 14% and initial curvilinear velocity of 89 ± 20 μm/s across 30 males were observed. Principal component analysis of sperm kinematic characteristics revealed distinct populations of sperm cells exhibiting varying responses to cryopreservation. The developed method achieved successful fertilization comparable to that of the non-frozen control group using sperm from a single cryotube (2.5 mL, approximately 50 * 109 spermatozoa) to fertilize 200 g of eggs (1:120,000 egg:spz).DiscussionThis novel approach demonstrates an effective cryopreservation protocol for common carp sperm in large-volume cryo-containers in combination with low-ionic cryomedia and high thawing temperature, providing methods well-suited for fisheries practices and selective breeding programs. Future studies of the biological properties of different sperm subpopulations in post-thaw sperm samples can contribute to a deeper understanding of sperm biology, improve cryopreservation techniques, and enhance the success rates of assisted reproductive technologies.

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Section: Discussionmentioning

confidence: 55%

Optimizing aquaculture-scale common carp artificial reproduction: a novel approach to sperm cryopreservation using large-volume containers and elevated thawing temperatures

Sotnikov,

Rodina,

Gela

et al. 2024

Front. Mar. Sci.

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“…On the other hand, it has been shown that optimization of sperm concentration plays a crucial role in a number of aquaculture species, in particular pikeperch (Sander lucioperca) [30], European perch (Perca fluviatilis) [9], and Salmonid [31]. However, it was observed that there was no significant difference between sperm samples' motility and velocity after thawing when different sperm concentrations were specially adjusted before freezing in carp sperm [32].…”

Section: Carp Sperm Cryopreservationmentioning

confidence: 99%

Cryopreservation Studies in Aquaculture from Past to Present: Scientific Techniques and Quality Controls for Commercial Applications

Ekici¹,

Yamaner²,

Demircan³

2023

Biomedical Engineering

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In this section, cryopreservation of fish genetic resources, which is one of the important applications to ensure the sustainability of genetic resources of freshwater fish species, is discussed. At the same time, information is provided about the possible sources of contamination that may be encountered during cryopreservation applications. In this context, the results of sperm, egg, and embryo cryopreservation studies of fish and their success and failure in applications were evaluated in addition to the process from past to present. Information is given about the contamination that may develop depending on the applications in the process of cryopreservation and dissolving processes, as well as the studies carried out to eliminate extracellular disease agents. In the section, in addition to the evaluation of the results of scientific studies, commercial companies that commercially carry out gamete cryopreservation applications are also included. The contamination that may develop depending on the applications in the process of cryopreservation and thawing processes, as well as the studies carried out to eliminate extracellular disease agents are mentioned.

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Adjustment of common carp sperm concentration prior to cryopreservation: Does it matter?

Cited by 2 publications

References 23 publications

Optimizing aquaculture-scale common carp artificial reproduction: a novel approach to sperm cryopreservation using large-volume containers and elevated thawing temperatures

Optimizing aquaculture-scale common carp artificial reproduction: a novel approach to sperm cryopreservation using large-volume containers and elevated thawing temperatures

Cryopreservation Studies in Aquaculture from Past to Present: Scientific Techniques and Quality Controls for Commercial Applications

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