Aditivos Mucogénicos para el Control de Caligus rogercresseyi en Salmón del Atlántico (Salmo salar)

Concha, Keila; Olivares, Pamela; Fonseca-Salamanca, Flery; Sánchez, Rodrigo; Serrano, Felipe; Parodí, Jorge

doi:10.15381/rivep.v28i3.13371

Cited by 3 publications

(1 citation statement)

References 19 publications

(33 reference statements)

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“…However, compounds used to prevent infection are ionic samples, like iodine. Iodine is used to generate fish mucus quality changes (Concha et al, 2017) and treat some salmonid problems (Mustafa & MacKinnon, 2011). The molecule also has broad effects on ion channels and receptors and may have a role in interacting with GABA (Abel et al, 1989).…”

Section: Figurementioning

confidence: 99%

A low‐cost screening system for kinetic analysis of Caligus rogercresseyi : New focus on pharmacological study of caligidosis disease

Parodí¹,

Hernández²,

Pizarro

et al. 2021

Aquaculture Research

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The study of the parasite Caligus rogercresseyi (sea louse) and its effects on salmon farms in Chile is a complex challenge. One of these problems concerns the cost for testing potential treatments' efficacy; the development of in vitro infection models is expensive because of the biosecurity protocol in general and animal care required. Our objective was to develop a low-cost system to evaluate the effect of different drugs on Caligus infestation. We previously used a low-cost software (ImageJ) to observe the kinetic parameters in Caligus sperm cells, which had been video recorded under an inverted microscope. The same was carried out for Caligus at different lifecycle stages (copepodites), where we used a modified protocol for ImageJ analysis (CASA) for kinetic parameter analysis. The Caligus were observed under both controlled (seawater at 15°), or experimental conditions (tap water dilution 0.1 at 100 V/V). Our results suggest that we can use the ImageJ plugin (CASA) as a low-cost system for screening potential drug compounds that have the potential to alter the physiology of Caligus rogercresseyi, as well as compare the pharmacological effects of a new drug before conducting an in vitro or in vivo study.

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Section: Figurementioning

confidence: 99%

A low‐cost screening system for kinetic analysis of Caligus rogercresseyi : New focus on pharmacological study of caligidosis disease

Parodí¹,

Hernández²,

Pizarro

et al. 2021

Aquaculture Research

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Structural and histochemical aspects of the Gymnotus carapo tegument (Teleostei: Gymnotiformes)

COHENE,

OLEA,

QUINTANA

2023

An. Acad. Bras. Ciênc.

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The present study aims to structurally and histochemically characterize the Gymnotus carapo tegument. 30 specimens were captured and slaughtered by spinal section with anesthesia. The observation was carried out with a stereoscopic microscope and the body surface was photographed. Fragments of the dorsal, ventral and lateral region were fixed in Bouin's solution for 12 hours and subsequently preserved in 70% alcohol. They were subsequently observed in the scanning electron microscope (SEM).The preparation for SEM was performed following the standardized protocol. Histological preparations were made, and the cuts were colored with H-E, PAS and Coomassie Blue. The images were obtained in an Olympus BX41-ENUTV-4 microscope. From the observations in SEM a plain tegument with pores of different sizes could be evidenced.The scales of the different regions of the body have different ornaments. Microscopically it was composed of a stratified non-keratinized epithelium consisting of two types of morphologically distinct cells: epidermal cells and mucous cells (PAS-Commassie Blue positive). Under the epithelium there is a layer of dense irregular connective tissue with associated chromatophores and more deeply scales. These analyzes are the basis for future studies that will focus on elucidating the events related to integumentary healing in this species.

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Evaluation of the Biological Activities of Peptides from Epidermal Mucus of Marine Fish Species from Chilean Aquaculture

Álvarez,

Toro-Araneda,

Cumillaf

et al. 2024

Marine Drugs

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The skin of fish is a physicochemical barrier that is characterized by being formed by cells that secrete molecules responsible for the first defense against pathogenic organisms. In this study, the biological activity of peptides from mucus of Seriola lalandi and Seriolella violacea were identified and characterized. To this purpose, peptide extraction was carried out from epidermal mucus samples of juveniles of both species, using chromatographic strategies for purification. Then, the peptide extracts were characterized to obtain the amino acid sequence by mass spectrometry. Using bioinformatics tools for predicting antimicrobial and antioxidant activity, 12 peptides were selected that were chemically produced by simultaneous synthesis using the Fmoc-Tbu strategy. The results revealed that the synthetic peptides presented a random coil or extended secondary structure. The analysis of antimicrobial activity allowed it to be discriminated that four peptides, named by their synthesis code 5065, 5069, 5070, and 5076, had the ability to inhibit the growth of Vibrio anguillarum and affected the copepodite stage of C. rogercresseyi. On the other hand, peptides 5066, 5067, 5070, and 5077 had the highest antioxidant capacity. Finally, peptides 5067, 5069, 5070, and 5076 were the most effective for inducing respiratory burst in fish leukocytes. The analysis of association between composition and biological function revealed that the antimicrobial activity depended on the presence of basic and aromatic amino acids, while the presence of cysteine residues increased the antioxidant activity of the peptides. Additionally, it was observed that those peptides that presented the highest antimicrobial capacity were those that also stimulated respiratory burst in leukocytes. This is the first work that demonstrates the presence of functional peptides in the epidermal mucus of Chilean marine fish, which provide different biological properties when the fish face opportunistic pathogens.

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Aditivos Mucogénicos para el Control de Caligus rogercresseyi en Salmón del Atlántico (Salmo salar)

Cited by 3 publications

References 19 publications

A low‐cost screening system for kinetic analysis of Caligus rogercresseyi : New focus on pharmacological study of caligidosis disease

A low‐cost screening system for kinetic analysis of Caligus rogercresseyi : New focus on pharmacological study of caligidosis disease

Structural and histochemical aspects of the Gymnotus carapo tegument (Teleostei: Gymnotiformes)

Evaluation of the Biological Activities of Peptides from Epidermal Mucus of Marine Fish Species from Chilean Aquaculture

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