Adipose-tissue engineering: Taking advantage of the properties of human adipose-derived stem/stromal cells

Vallée, Maud; Côté, Julie-Anne; Fradette, Julie

doi:10.1016/j.patbio.2008.04.010

Cited by 60 publications

(46 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1 Human adipose tissue-derived stem cells (hASCs) isolated from the stromal vascular fraction (SVF) of the adipose tissue bear resemblances to bone marrow-derived mesenchymal cells 2,3 demonstrated by their similar morphology, and common surface markers and gene expression profiles. [4][5][6] However, the SVF of the adipose tissue harbors more than 2% of cells featuring potential for multilineage differentiation compared to the 0.002% of the bone marrow.…”

Section: Introductionmentioning

confidence: 99%

“…[4][5][6] However, the SVF of the adipose tissue harbors more than 2% of cells featuring potential for multilineage differentiation compared to the 0.002% of the bone marrow. 1,4,7 Additionally, a large number of studies have proven the hASCs differentiation potential towards multiple lineages, namely the osteogenic, 8 chondrogenic, 8 adipogenic, 8 myogenic, 8 and neurogenic. 9 Also, the developmental plasticity of hASCs was demonstrated both in vitro 10 and in vivo.…”

Section: Introductionmentioning

confidence: 99%

“…1 Although there has been extensive research effort to create functional-engineered tissues, the success of such approaches still relies on the construction of vascular networks capable of delivering oxygen and nutrients within the engineered constructs. 12 Thus, in the context of bone engineering, the development of strategies that could effectively induce the microcirculation within the engineered constructs has become a major pursuit.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Human Adipose Tissue-Derived SSEA-4 Subpopulation Multi-Differentiation Potential Towards the Endothelial and Osteogenic Lineages

Mihăilă

Frias

Pirraco

et al. 2013

Tissue Engineering Part A

View full text Add to dashboard Cite

Human adipose tissue has been recently recognized as a potential source of stem cells for regenerative medicine applications, including bone tissue engineering (TE). Despite the gathered knowledge regarding the differentiation potential of human adipose tissue-derived stem cells (hASCs), in what concerns the endothelial lineage many uncertainties are still present. The existence of a cell subpopulation within the human adipose tissue that expresses a SSEA-4 marker, usually associated to pluripotency, raises expectations on the differentiation capacity of these cells (SSEA-4 + hASCs). In the present study, the endothelial and osteogenic differentiation potential of the SSEA-4 + hASCs was analyzed, aiming at proposing a single-cell source/ subpopulation for the development of vascularized bone TE constructs. SSEA-4 + hASCs were isolated using immunomagnetic sorting and cultured either in a-MEM, in EGM-2 MV (endothelial growth medium), or in osteogenic medium. SSEA-4 + hASCs cultured in EGM-2 MV formed endothelial cell-like colonies characterized by a cobblestone morphology and expression of CD31, CD34, CD105, and von Willebrand factor as determined by quantitative reverse transcriptase (RT)-polymerase chain reaction, immunofluorescence, and flow cytometry. The endothelial phenotype was also confirmed by their ability to incorporate acetylated lowdensity lipoprotein and to form capillary-like structures when seeded on Matrigel. SSEA-4+ hASCs cultured in a-MEM displayed fibroblastic-like morphology and exhibited a mesenchymal surface marker profile (>90% CD90+ ). After culture in osteogenic conditions, an overexpression of osteogenic-related markers (osteopontin and osteocalcin) was observed both at molecular and protein levels. Matrix mineralization detected by Alizarin Red staining confirmed SSEA-4 + hASCs osteogenic differentiation. Herein, we demonstrate that from a single-cell source, human adipose tissue, and by selecting the appropriate subpopulation it is possible to obtain microvascular-like endothelial cells and osteoblasts, the most relevant cell types for the creation of vascularized bone tissue-engineered constructs.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Human Adipose Tissue-Derived SSEA-4 Subpopulation Multi-Differentiation Potential Towards the Endothelial and Osteogenic Lineages

Mihăilă

Frias

Pirraco

et al. 2013

Tissue Engineering Part A

View full text Add to dashboard Cite

show abstract

“…For example, MSCs are a rare population in bone marrow constituting only 0.002% of total stromal cell population. 14,15 Whereas adipose tissue harbors nearly 2% MSCs in its stromal vascular fraction. 14,15 As compared to bone marrow, further reduced frequency of MSCs is reported for cord blood stem cells.…”

Section: Introductionmentioning

confidence: 99%

“…14,15 Whereas adipose tissue harbors nearly 2% MSCs in its stromal vascular fraction. 14,15 As compared to bone marrow, further reduced frequency of MSCs is reported for cord blood stem cells. 16, 17 Gronthos et al 10 reported that colonies of dental pulp cells occur at an apparently higher frequency in comparison to bone marrow over similar plating densities.…”

Section: Introductionmentioning

confidence: 99%

Clinical grade adult stem cell banking

2009

View full text Add to dashboard Cite

A Cell‐Based Self‐Assembly Approach for the Production of Human Osseous Tissues from Adipose‐Derived Stromal/Stem Cells

Galbraith

Clafshenkel

Kawecki

et al. 2016

Adv Healthcare Materials

View full text Add to dashboard Cite

Achieving optimal bone defect repair is a clinical challenge driving intensive research in the field of bone tissue engineering. Many strategies focus on seeding graft materials with progenitor cells prior to in vivo implantation. Given the benefits of closely mimicking tissue structure and function with natural materials, the authors hypothesize that under specific culture conditions, human adipose-derived stem/stromal cells (hASCs) can solely be used to engineer human reconstructed osseous tissues (hROTs) by undergoing osteoblastic differentiation with concomitant extracellular matrix production and mineralization. Therefore, the authors are developing a self-assembly methodology allowing the production of such osseous tissues. Three-dimensional (3D) tissues reconstructed from osteogenically-induced cell sheets contain abundant collagen type I and are 2.7-fold less contractile compared to non-osteogenically induced tissues. In particular, hROT differentiation and mineralization is reflected by a greater amount of homogenously distributed alkaline phosphatase, as well as higher calcium-containing hydroxyapatite (P < 0.0001) and osteocalcin (P < 0.0001) levels compared to non-induced tissues. Taken together, these findings show that hASC-driven tissue engineering leads to hROTs that demonstrate structural and functional characteristics similar to native osseous tissue. These highly biomimetic human osseous tissues will advantageously serve as a platform for molecular studies as well as for future therapeutic in vivo translation.

show abstract

Adipose-tissue engineering: Taking advantage of the properties of human adipose-derived stem/stromal cells

Cited by 60 publications

References 38 publications

Human Adipose Tissue-Derived SSEA-4 Subpopulation Multi-Differentiation Potential Towards the Endothelial and Osteogenic Lineages

Human Adipose Tissue-Derived SSEA-4 Subpopulation Multi-Differentiation Potential Towards the Endothelial and Osteogenic Lineages

Clinical grade adult stem cell banking

A Cell‐Based Self‐Assembly Approach for the Production of Human Osseous Tissues from Adipose‐Derived Stromal/Stem Cells

Contact Info

Product

Resources

About