Adipose tissue-derived stem cells: a comparative review on isolation, culture, and differentiation methods

Khazaei, Saber; Keshavarz, Ghazal; Bozorgi, Azam; Nazari, Hamed; Khazaei, Mozafar

doi:10.1007/s10561-021-09905-z

Cited by 33 publications

(26 citation statements)

References 113 publications

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“…In the last 15 years, the role of stem cells has given a major boost to various dental regenerative procedures for repairing dental and other oral defects [28][29][30]. However, all these procedures require aseptic conditions, which is greatly altered by the presence of pathogenic oral microorganisms.…”

Section: Discussionmentioning

confidence: 99%

Synergistic Effect of Plant Extracts on Endodontic Pathogens Isolated from Teeth with Root Canal Treatment Failure: An In Vitro Study

et al. 2021

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Background and objectives: This study aimed to evaluate the synergistic antimicrobial activity of extracts obtained from Salvadora persica (Miswak), Commiphora molmol (myrrh) and Azadirachta indica (neem) in combination with commercially available antimicrobial agents: penicillin, tetracycline, ofloxacin and fluconazole on endodontic pathogens such as Enterococcus faecalis, Streptococcus mitis, Actinomyces naeslundii and Candida albicans. Materials and Methods: Microbiological samples from the root canals of the teeth undergoing retreatment were taken using sterile paper points kept at full length in the canal for 30 seconds. The disc diffusion method was used to check the susceptibility of microbes to the plant extracts and antimicrobials by measuring the diameter of the inhibition zones. Against the microbes, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC)/minimum fungicidal concentration (MFC) of the plant extracts were assessed. The fractional inhibitory concentration index (FICI) was used to estimate the synergistic effect of plant extracts combined with antimicrobials against the resistant endodontic microbes. Results: The findings clearly indicate the effectiveness of all the three plant extracts, Commiphora molmol, Azadirachta indica, Salvadora persica, against all the experimental pathogenic microorganisms except for the ineffectiveness of Azadirachta indica, Salvadora persica against Candida albicans. Maximum antimicrobial activity was displayed by Azadirachta indica against Enterococcus faecalis (MIC = 0.09 ± 1.2 mg/mL, MBC = 0.78 ± 1.25 mg/mL) and the minimum antimicrobial activity was displayed by Commiphora molmol against Actinomyces naeslundii (MIC = 12.5 ± 3.25 mg/mL, MBC = 100 ± 3.75 mg/mL). The best synergy was displayed by Commiphora molmol with fluconazole against Candida albicans (FICI = 0.45). Conclusions: The current study delineates the variable antimicrobial activity of plant extracts against the experimental endodontic pathogenic microorganisms. Plant extracts in conjunction with various antimicrobials can be valuable aids in combating relatively resistant endodontic microorganisms that have been the cause of worry in recent years, leading to failure even in treatment procedures following all required protocols.

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Section: Discussionmentioning

confidence: 99%

Synergistic Effect of Plant Extracts on Endodontic Pathogens Isolated from Teeth with Root Canal Treatment Failure: An In Vitro Study

et al. 2021

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“…The 10/30 fraction contained immature osteoblasts, the 50/70 fraction contained mature osteoblasts, and the 30/50 fraction contained intermediate osteoblasts during the differentiation process. In the plastic surgery field, the evaluation of the enrichment of adipose tissue‐derived stem cells or adipose stromal/stem cells has been based on centrifugation and density 41 . Therefore, this technique can be applied to analyze adipocyte differentiation stages in the future.…”

Section: Discussionmentioning

confidence: 99%

“…In the plastic surgery field, the evaluation of the enrichment of adipose tissue-derived stem cells or adipose stromal/stem cells has been based on centrifugation and density. 41 Therefore, this technique can be applied to analyze adipocyte differentiation stages in the future.…”

Section: Expression Analysis Of Cell Surface Markers In Three Fractionsmentioning

confidence: 99%

Intracellular density is a novel indicator of differentiation stages of murine osteoblast lineage cells

Itoh

Naruse

et al. 2021

J of Cellular Biochemistry

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Osteoblasts are primary bone‐making cells originating from mesenchymal stem cells (MSCs) in the bone marrow. The differentiation of MSCs to mature osteoblasts involves an intermediate stage called preosteoblasts, but the details of this process remain unclear. This study focused on the intracellular density of immature osteoblast lineage cells and hypothesized that the density might vary during differentiation and might be associated with the differentiation stages of osteoblast lineage cells. This study aimed to clarify the relationship between intracellular density and differentiation stages using density gradient centrifugation. Primary murine bone marrow stromal cell cultures were prepared in an osteogenic induction medium, and cells were separated into three fractions (low, intermediate, and high‐density). The high‐density fraction showed elevated expression of osteoblast differentiation markers (Sp7, Col1a1, Spp1, and Bglap) and low expression of MSC surface markers (Sca‐1, CD73, CD105, and CD106). In contrast, the low‐density fraction showed a high expression of MSC surface markers. These results indicated that intracellular density increased during differentiation from preosteoblasts to committed osteoblasts. Intracellular density may be a novel indicator for osteoblast differentiation stages. Density gradient centrifugation is a novel technique to study the process by which preosteoblasts transform into bone‐forming cells.

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“…O tecido branco é predominante em Sumário adultos, localiza-se abaixo da pele e apresenta células de gordura com uma única gota lipídica intracelular. Esse tecido é responsável pelo armazenamento de energia e pela liberação de substâncias que participam de diversas vias no organismo (LIN et al, 2021;KHAZAEI et al, 2021). O tecido adiposo marrom é observado em recém-nascidos, possui várias pequenas gotículas de gordura e um número considerável de mitocôndrias e é responsável pelo controle térmico, liberando ácidos graxos armazenados e glicose.…”

Section: O Tecido Adiposounclassified

Covid dezenove em pacientes obesos

amaral¹,

filho²,

gonçalves³

2021

Obesidade E Covid Dezenove

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Adipose tissue-derived stem cells: a comparative review on isolation, culture, and differentiation methods

Cited by 33 publications

References 113 publications

Synergistic Effect of Plant Extracts on Endodontic Pathogens Isolated from Teeth with Root Canal Treatment Failure: An In Vitro Study

Synergistic Effect of Plant Extracts on Endodontic Pathogens Isolated from Teeth with Root Canal Treatment Failure: An In Vitro Study

Intracellular density is a novel indicator of differentiation stages of murine osteoblast lineage cells

Covid dezenove em pacientes obesos

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