Adipose Tissue-Derived Mesenchymal Stem Cells as a New Host Cell in Latent Leishmaniasis

Abstract

Some protozoan infections such as Toxoplasma, Cryptosporidium, and Plasmodium can be transmitted through stem cell transplantations. To our knowledge, so far, there is no study about transmission of Leishmania parasites in stem cell transplantation and interactions between parasites and stem cells in vitro. Therefore, the aim of this study was to investigate the interaction between different species of Leishmania parasites and adipose tissue-derived mesenchymal stem cells (ADMSCs). ADMSCs have been isolated, cultured, characterized, and infected with different species of Leishmania parasites (L. donovani, L. major, L. tropica, and L. infantum). Infectivity was examined by Giemsa staining, microculture, and polymerase chain reaction methods. As a result, infectivity of ADMSCs by Leishmania parasites has been determined for the first time in this study. According to our findings, it is very important that donors are screened for Leishmania parasites before stem cell transplantations in regions where leishmaniasis is endemic.

Mesenchymal stem cells and infectious diseases: Smarter than drugs

“…AMSCs for instance can be infected with various types of Leishmania parasites [65]. IFN-␥ and TNF-␣ costimulated human and murine infected BMSCs are able to eliminate the obligate intracellular parasite: type II Toxoplasma gondii, by upregulating IDO or iNOS expression, respectively.…”

Pretreatment of Mesenchymal Stem Cells WithLeishmania majorSoluble Antigens Induce Anti-Inflammatory Properties in Mouse Peritoneal Macrophages

“…It is noted that MSCs are infected by L . species [Allahverdiyev et al, ]; however, there is no evidence about MSCs immunomodulatory behaviors in L. major ‐infected tissue. Therefore, we intend to assess the effects of the soluble L. major antigens primed MSCs on macrophage immune responses to lipopolysaccharide (LPS) in both cell‐to‐cell contact‐dependent manner and conditioned media (CM) treatment.…”

“…In a previous study, we have showed an increase in transforming growth factor-alpha (TNF)-a/IL-10 ratio after co-culture of MSCs with macrophages in BALB/c and C57BL/6 mice in response to Leishmania major (L. major) infection, indicating a positive effect in treatment of leishmaniasis [Dameshghi et al, 2015]. It is noted that MSCs are infected by L. species [Allahverdiyev et al, 2011]; however, there is no evidence about MSCs immunomodulatory behaviors in L. major-infected tissue. Therefore, we intend to assess the effects of the soluble L. major antigens primed MSCs on macrophage immune responses to lipopolysaccharide (LPS) in both cell-to-cell contactdependent manner and conditioned media (CM) treatment.…”

Utility of the Microculture Method in Non-Invasive Samples Obtained from an Experimental Murine Model with Asymptomatic Leishmaniasis

“…In all assays, positive controls containing L. donovani DNA and a negative control without DNA were included; 10 μL reaction mixture were visualized by 2% agarose gel electrophoresis. 24 Our previously experiments showed that the analytical sensitivity of PCR method was 100 parasites in evaluated samples (unpublished results).…”

“…The sample was centrifuged at 15,800 + g for 10 minutes, and the pellet was washed with cold 70% ethanol and then dissolved in 20 μL TE buffer 10/0.1 (10 mM Tris·HCl, 0.1 mM EDTA, pH 8.0). 24 Samples for diagnosis by PCR. PCR has been performed for the amplification of Leishmania-specific gene from miniexon sequences by using DNA extracted from previously infected BALB/c PB and MCM samples.…”