Adipose tissue derived mesenchymal stem cell (AD-MSC) promotes skin wound healing in diabetic rats

Khosravi‐Maharlooei, Mohsen; Bagheri, Mansooreh; Solhjou, Zhabiz; Jahromi, Behnam Moein; Akrami, Majid; Rohani, Lili; Monabati, Ahmad; Noorafshan, Ali; Omrani, Gholamhossein Ranjbar

doi:10.1016/j.diabres.2011.04.018

Cited by 140 publications

(94 citation statements)

References 29 publications

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“…Blood sugar level was recorded using Accu Check glucometer before and after administration of Streptozotocin (Normal blood sugar level recorded 80-100 mg/dl). Rats with blood sugar level more than 70 280 mg/dl ( Figure 2) and signs of polydipsia and ketonuria were considered as diabetic [11]. After sacrificing the rats, pancreas was collected in 3.7% buffered formalin for fixing.…”

Section: Diabetes Inductionmentioning

confidence: 99%

Therapeutic Potential of Canine Bone Marrow Derived Mesenchymal Stem Cells and its Conditioned Media in Diabetic Rat Wound Healing

Ansari¹,

Tr²,

Ch³

et al. 2013

J Stem Cell Res Ther

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Present study was conducted to explore the possibility of treating the diabetic rat wounds by canine bone marrow derived mesenchymal stem cells (BMSCs) and its conditioned media (CM). Canine bone marrow stem cells were isolated ascetically from dog and cultured in vitro. Stem cells conditioned media was collected from third passage cells at 120 hours of culture. Streptozotocin was used for induction of diabetes in rats. Six groups were made for the wound healing therapy in which group I, II and III were non-diabetic while group IV, V and VI were diabetic with six animals in each group and one wound was created in each animal. Group II and V received stem cells in culture media and group III and VI received conditioned media while group I and IV were kept as respective controls given only stem cell culture media. Stem cells and its conditioned media were injected at the periphery of wounds. Wound healing was assessed by wound contraction, photographic evaluation at different time interval (0, th day) and histomorphological examination on day 28. The outcomes of wound healing experiment suggested that canine bone marrow stem cells as well as its conditioned media can be exploited xenogenically very well for diabetic rat wound healing.

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Section: Diabetes Inductionmentioning

confidence: 99%

Therapeutic Potential of Canine Bone Marrow Derived Mesenchymal Stem Cells and its Conditioned Media in Diabetic Rat Wound Healing

Ansari¹,

Tr²,

Ch³

et al. 2013

J Stem Cell Res Ther

View full text Add to dashboard Cite

show abstract

“…54 While angiogenin and VEGF-A secretion levels were comparable within MSCs populations, DSCs and DPCs released a significantly higher amount of leptin. These angiogenic features were confirmed in excisional 26,57 and chronic wounds 58 healing, and they can be maximized by playing with the oxygen concentration, as discussed in section ''Hypoxia.'' MSCs secretome can further act in the remodeling phase of cutaneous wound healing.…”

Section: Secretome Unitsmentioning

confidence: 85%

Stem Cells in Skin Wound Healing: Are We There Yet?

Cerqueira

Pirraco

Marques

2016

Advances in Wound Care

101

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“…In the non-MSCs group collagen density exceeded the density of collagen in the NS-group. Maharlooei et al evaluated the volume density of collagen fibres in a control group and treatment group in a diabetic rat model where a full-thickness wound was made on the dorsum of the rat (n=26) [25]. No significant differences were found between both groups in volume density of collagen fibres, however, they showed a higher wound healing rate in rats with diabetes.…”

Section: Discussionmentioning

confidence: 99%