Adipose Tissue and Lipid Metabolism

Buyse, Johan; Decuypere, Eddy

doi:10.1016/b978-0-12-407160-5.00019-1

Cited by 19 publications

(25 citation statements)

References 66 publications

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“…Visceral fat is an important endocrine organ. 1,2 The results of sandwich ELISA demonstrated that Adamts18 KO mice showed significantly increased serum LEP level relative to WT littermates (KO versus WT, 5.69 AE 0.78 versus 4.33 AE 0.77 mg/L; P Z 0.01; n Z 7 per group) ( Figure 6A). Furthermore, Adamts18 KO mice showed a significantly decreased serum INS level relative to WT littermates (KO versus WT, 10.2 AE 1.6 versus 12.5 AE 1.5 mU/L; P Z 0.026; n Z 7 per group) ( Figure 6B).…”

Section: Adamts18 Deficiency Causes Abnormal Blood Glu Metabolism In mentioning

confidence: 99%

“…Increased visceral fat volume is a major contributory factor in hyperlipidemia, because excessive TG is continually released by fat cells into the bloodstream. 1,2 To further investigate the relevance of ADAMTS18 and hyperlipidemia, the blood lipid level of mice was examined. Compared with WT controls, Adamts18 KO mice fed a regular chow diet showed increased LDL (KO versus WT, 3.56 AE 0.28 versus 3.25 AE 0.22 mmol/L; P Z 0.0408), decreased HDL (KO versus WT, 1.30 AE 0.09 versus 1.48 AE 0.15 mmol/L; P Z 0.018), increased TG (KO versus WT, 1.31 AE 0.07 versus 0.84 AE 0.09 mmol/L; P < 0.001), and increased TC (KO versus WT, 4.79 AE 0.43 versus 4.1 AE 0.33 mmol/L; P Z 0.0058; n Z 7 per group) ( Figure 5A).…”

Section: Adamts18 Deficiency Causes Increased Serum Lipid Levels In Micementioning

confidence: 99%

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A Disintegrin and Metalloproteinase with Thrombospondin Motifs 18 Deficiency Leads to Visceral Adiposity and Associated Metabolic Syndrome in Mice

Zhu

Cheng

et al. 2018

The American Journal of Pathology

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Visceral adiposity is of greater risk than obesity in s.c. adipose tissue for diabetes and cardiovascular disease. Its pathogenesis remains unclear, but it is associated with extracellular matrix (ECM) remodeling. A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTSs) are a family of secreted zinc-dependent metalloproteinases that play crucial roles in development and various diseases because of their ECM remodeling activity. ADAMTS18 is an orphan ADAMTS whose function and substrate remain unclear. Herein, we showed that Adamts18 mRNA was abundantly expressed in visceral (gonadal) white adipose tissue (vWAT) during the early stage of development after birth. Adamts18 knockout (KO) mice showed increased body fat percentage and larger adipocyte size in vWAT relative to wild-type littermates. These findings may be partly attributed to ECM remodeling, especially increased expression of laminin 1 and adipokine thrombospondin 1 in vWAT. Attenuated extracellular signal-regulated kinase 1 and 2 activity, along with increased expression of adipocyte-specific transcription factors peroxisome proliferator-activated receptor-γ, CCAAT/enhancer binding protein β, and marker gene Fabp4, was detected in vWAT of Adamts18 KO mice. Furthermore, Adamts18 KO mice showed early metabolic syndrome, including hyperlipidemia, blood glucose metabolic disorder, and hypertension. ADAMTS18 deficiency promotes atherosclerosis in apolipoprotein E-deficient mice. These results indicate a novel function of ADAMTS18 in vWAT development and associated metabolic disorders.

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Section: Adamts18 Deficiency Causes Abnormal Blood Glu Metabolism In mentioning

confidence: 99%

Section: Adamts18 Deficiency Causes Increased Serum Lipid Levels In Micementioning

confidence: 99%

A Disintegrin and Metalloproteinase with Thrombospondin Motifs 18 Deficiency Leads to Visceral Adiposity and Associated Metabolic Syndrome in Mice

Zhu

Cheng

et al. 2018

The American Journal of Pathology

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“…This result highlights a possible additional production site of T3 behind the mediobasal hypothalamus [79]. It has also been proposed that T3 stimulates hepatic malic enzyme activity contributing to lipogenesis [80].…”

Section: Discussionmentioning

confidence: 90%

Integrative analysis of transcriptomic data related to the liver of laying hens: from physiological basics to newly identified functions

et al. 2019

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BackgroundAt sexual maturity, the liver of laying hens undergoes many metabolic changes to support vitellogenesis. In published transcriptomic approaches, hundreds of genes were reported to be overexpressed in laying hens and functional gene annotation using gene ontology tools have essentially revealed an enrichment in lipid and protein metabolisms. We reanalyzed some data from a previously published article comparing 38-week old versus 10-week old hens to give a more integrative view of the functions stimulated in the liver at sexual maturity and to move beyond current physiological knowledge. Functions were defined based on information available in Uniprot database and published literature.ResultsOf the 516 genes previously shown to be overexpressed in the liver of laying hens, 475 were intracellular (1.23–50.72 fold changes), while only 36 were predicted to be secreted (1.35–66.93 fold changes) and 5 had no related information on their cellular location. Besides lipogenesis and protein metabolism, we demonstrated that the liver of laying hens overexpresses several clock genes (which supports the circadian control of liver metabolic functions) and was likely to be involved in a liver/brain/liver circuit (neurotransmitter transport), in thyroid and steroid hormones metabolisms. Many genes were associated with anatomical structure development, organ homeostasis but also regulation of blood pressure. As expected, several secreted proteins are incorporated in yolky follicles but we also evidenced that some proteins are likely participating in fertilization (ZP1, MFGE8, LINC00954, OVOCH1) and in thyroid hormone maturation (CPQ). We also proposed that secreted proteins (PHOSPHO1, FGF23, BMP7 but also vitamin-binding proteins) may contribute to the development of peripheral organs including the formation of medullar bones to provide labile calcium for eggshell formation. Thirteen genes are uniquely found in chicken/bird but not in human species, which strengthens that some of these genes may be specifically related to avian reproduction.ConclusionsThis study gives additional hypotheses on some molecular actors and mechanisms that are involved in basic physiological function of the liver at sexual maturity of hen. It also revealed some additional functions that accompany reproductive capacities of laying hens, and that are usually underestimated when using classical gene ontology approaches.

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“…It is notable that white and brown adipose tissue showed differences in their pattern of MAG species, which may explain functional differences between them. For example, white and brown adipose tissues differ significantly based on their structure and physiology [ 27 , 28 ]. Specifically, the primary purpose of white adipose tissue is to store and release energy in the form of free fatty acids, while the essential function executed by brown adipose tissue is the expenditure of fatty acid-derived energy for maintenance of the organism’s thermal stability [ 29 , 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Monoacylglycerol Analysis Using MS/MSALL Quadruple Time of Flight Mass Spectrometry

Gao¹,

McDaniel²,

Chen³

et al. 2016

Metabolites

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Monoacylglycerols (MAGs) are structural and bioactive metabolites critical for biological function. Development of facile tools for measuring MAG are essential to understand its role in different diseases and various pathways. A data-independent acquisition method, MS/MSALL, using electrospray ionization (ESI) coupled quadrupole time of flight mass spectrometry (MS), was utilized for the structural identification and quantitative analysis of individual MAG molecular species. Compared with other acylglycerols, diacylglycerols (DAG) and triacylglycerols (TAG), MAG characteristically presented as a dominant protonated ion, [M + H]+, and under low collision energy as fatty acid-like fragments due to the neutral loss of the glycerol head group. At low concentrations (<10 pmol/µL), where lipid-lipid interactions are rare, there was a strong linear correlation between ion abundance and MAG concentration. Moreover, using the MS/MSALL method the major MAG species from human plasma and mouse brown and white adipose tissues were quantified in less than 6 min. Collectively, these results demonstrate that MS/MSALL analysis of MAG is an enabling strategy for the direct identification and quantitative analysis of low level MAG species from biological samples with high throughput and sensitivity.

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Adipose Tissue and Lipid Metabolism

Cited by 19 publications

References 66 publications

A Disintegrin and Metalloproteinase with Thrombospondin Motifs 18 Deficiency Leads to Visceral Adiposity and Associated Metabolic Syndrome in Mice

A Disintegrin and Metalloproteinase with Thrombospondin Motifs 18 Deficiency Leads to Visceral Adiposity and Associated Metabolic Syndrome in Mice

Integrative analysis of transcriptomic data related to the liver of laying hens: from physiological basics to newly identified functions

Monoacylglycerol Analysis Using MS/MSALL Quadruple Time of Flight Mass Spectrometry

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