Adipose Tissue and Extracellular Matrix Development by Injectable Decellularized Adipose Matrix Loaded with Basic Fibroblast Growth Factor

Abstract

This study demonstrates the high efficiency of heparinized decellularized adipose tissue matrix loaded with bFGF in promoting adipose neotissue formation and neovascularization with long-term volume stability.

Design of Injectable Materials to Improve Stem Cell Transplantation

“…Injectable materials that utilize decellularized matrix offer plenty of cell-binding sites necessary to anchor transplanted cells and prevent apoptosis. Furthermore, decellularized matrix can be harvested from the donor tissue of interest providing appropriate, tissue-specific biochemical cues necessary for transplanted cell engraftment and function [4, 38, 60, 61]. Alternatively, synthetic scaffolds can be decorated with known matrix ligands to elicit specific interactions with cell-surface receptors [62–68].…”

Hydrogels in adipose tissue engineering—Potential application in post‐mastectomy breast regeneration

“…When these were harvested at 3 weeks, the graft demonstrated well‐organised adipose tissue, demonstrating that a cell‐free scaffold can actively recruit cells from native tissues for the purposes of adipose tissue engineering (Kim, Choi, & Cho, ). Zhang, Lu, Cao, and Toh () investigated the adipogenic potential of DAT loaded with basic fibroblast growth factor as in injectable hydrogel in vivo. New adipose tissue formed with significant adipose tissue growth and neovascularisation was observed between 6 and 12 weeks after injection.…”

“…New adipose tissue formed with significant adipose tissue growth and neovascularisation was observed between 6 and 12 weeks after injection. In contrast, DAT without basic fibroblast growth factor was completely resorbed at 12 weeks (Zhang et al, ). Young, Ibrahim, Hu, & Christman () has also studied DAT as an acellular scaffold for adipose tissue regeneration; however, they do suggest that DAT as a hydrogel could be used to deliver human ADSCs to the defect site with greater concentration and consistency than current lipotransfer methods and thus achieve greater reconstructive success than lipotransfer due to the prevention of ADSC migration.…”

Current Therapeutic Strategies for Adipose Tissue Defects/Repair Using Engineered Biomaterials and Biomolecule Formulations

“…Multi-component materials are becoming a common approach in recent years producing interesting results. Basic fibroblast growth factor-2 (FGF-2) ( Kawaguchi et al, 1998 ; Kimura et al, 2003 ; Vashi et al, 2006 ; Zhang et al, 2016 ), fibroblast growth factor-1 (FGF-1) ( Moya et al, 2010 ), dexamethasone ( Rubin et al, 2009 ; Sun et al, 2013 ; Fan et al, 2015 ; Jia et al, 2015 ; Kelmendi-Doko et al, 2017 ), adipose-derived stem cells (ASCs) ( Wang et al, 2013 ; Cheung et al, 2014 ; Brown et al, 2015 ), pioglitazone ( Yazawa et al, 2015 ), insulin ( Masuda et al, 2004 ; Rubin et al, 2009 ; Hong et al, 2010 ), and insulin-like growth factor-1 (IGF-1) ( Masuda et al, 2004 ) are among several additive components that have been evaluated in vitro / in vivo . Several methods of encapsulation have been used to localize the delivery of the therapeutic agents.…”