Adipose-Derived Stromal/Stem Cells from Large Animal Models: from Basic to Applied Science

Bukowska, Joanna; Szóstek‐Mioduchowska, Anna; Kopcewicz, Marta; Walendzik, Katarzyna; Machcińska, Sylwia; Gawrońska‐Kozak, Barbara

doi:10.1007/s12015-020-10049-y

Cited by 25 publications

(21 citation statements)

References 152 publications

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“…Several studies have demonstrated the plasticity of human and animal ASCs [62][63][64]. Consistently, there is extensive literature regarding the safety and efficacy of ASC-based therapies for the treatment of numerous diseases and injuries created in animal models of human diseases, including cardiovascular disease and musculoskeletal and cutaneous injuries [65]. The therapeutic potential of ASCs or Stromal Vascular Fraction cells (SVFs) has been confirmed not only in experimental animal models but also in an increasing number of human clinical trials [66][67][68].…”

Section: Stem Cell Types For Fibrotic Disorders Therapymentioning

confidence: 87%

Perspective on Stem Cell Therapy in Organ Fibrosis: Animal Models and Human Studies

Bukowska

Sadowska

Wójtowicz

et al. 2021

Life

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Tissue fibrosis is characterized by excessive deposition of extracellular matrix (ECM) components that result from the disruption of regulatory processes responsible for ECM synthesis, deposition, and remodeling. Fibrosis develops in response to a trigger or injury and can occur in nearly all organs of the body. Thus, fibrosis leads to severe pathological conditions that disrupt organ architecture and cause loss of function. It has been estimated that severe fibrotic disorders are responsible for up to one-third of deaths worldwide. Although intensive research on the development of new strategies for fibrosis treatment has been carried out, therapeutic approaches remain limited. Since stem cells, especially mesenchymal stem cells (MSCs), show remarkable self-renewal, differentiation, and immunomodulatory capacity, they have been intensively tested in preclinical studies and clinical trials as a potential tool to slow down the progression of fibrosis and improve the quality of life of patients with fibrotic disorders. In this review, we summarize in vitro studies, preclinical studies performed on animal models of human fibrotic diseases, and recent clinical trials on the efficacy of allogeneic and autologous stem cell applications in severe types of fibrosis that develop in lungs, liver, heart, kidney, uterus, and skin. Although the results of the studies seem to be encouraging, there are many aspects of cell-based therapy, including the cell source, dose, administration route and frequency, timing of delivery, and long-term safety, that remain open areas for future investigation. We also discuss the contemporary status, challenges, and future perspectives of stem cell transplantation for therapeutic options in fibrotic diseases as well as we present recent patents for stem cell-based therapies in organ fibrosis.

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Section: Stem Cell Types For Fibrotic Disorders Therapymentioning

confidence: 87%

Perspective on Stem Cell Therapy in Organ Fibrosis: Animal Models and Human Studies

Bukowska

Sadowska

Wójtowicz

et al. 2021

Life

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“…In this study, ACMs different in morphology and physical structure have been evaluated in vitro and after subcutaneous implant in mice. Firstly, ACMs were incubated with ASCs to assess the interaction between the acellular scaffold and mesenchymal stem cells, representing one of the cellular elements involved in regeneration and repair pathways [27]. According to the literature, the stimulation and differentiation of ASCs increase wound repair and regeneration ability by intensifying collagen secretion and deposition [9,14].…”

Section: Discussionmentioning

confidence: 99%

Tissue-Material Integration and Biostimulation Study of Collagen Acellular Matrices

Sierra

Busato

Zingaretti

et al. 2022

Tissue Eng Regen Med

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Background: Breast reconstruction after mastectomy using silicone implants is a surgical procedure that occasionally leads to capsular contracture formation. This phenomenon constitutes an important and persistent cause of morbidity, and no successful therapies are available to date. Recently, the use of acellular membranes as a protective material for silicone prostheses has been gaining attention due to their ability to prevent this adverse outcome. For this reason, the evaluation of the tissue-material integration and the induced biostimulation by acellular membranes results crucial. Evaluation of in vivo tissue integration and biostimulation induced by three different natural acellular collagen membranes. Methods: Scanning electron microscopy was performed to analyse the membrane porosity and cells-biomaterial interaction in vitro, both in dry and wet conditions. Adipose-derived stem cells were cultured in the presence of membranes, and the colonisation capacity and differentiation potential of cells were assessed. In vivo tests and ex vivo analyses have been performed to evaluate dermal integration, absorption degree and biostimulation induced by the evaluated membrane. Results: Analysis performed in vitro on the three different acellular dermal matrices evidenced that porosity and the morphological structure of membranes influence the liquid swelling ratio, affecting the cell mobility and the colonisation capacity. Moreover, the evaluated membranes influenced in different manner the adipose derived stem cells differentiation and their survival. In vivo investigation indicated that the absorption degree and the fluid accumulation surrounding the implant were membrane-dependent. Finally, ex vivo analysis confirmed the membrane-dependent behavior revealing different degree of tissue integration and biostimulation, such as adipogenic stimulation. Conclusion: The physico-chemical characteristics of the membranes play a key role in the biostimulation of the cellular environment inducing the development of well-organized adipose tissue.

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“…The cellular components of AdSVF can be resuspended in platelet-rich plasma (PRP), phosphate-buffered saline (PBS), or 0.9% sodium chloride (saline) to produce a ready-to-use solution (Bukowska et al. 2021 ). In addition, AdSVF can be expanded in vitro to obtain AdSVF-derived mesenchymal stem cells that differentiate into diverse lineages of cells (Han et al.…”

Section: Preparation Of Adipose-derived Stromal Vascular Fractionmentioning

confidence: 99%

“…Reproduced from Bukowska et al. ( 2021 ) under Creative Commons Attribution 4.0 International License ( https://creativecommons.org/licenses/by/4.0/ ).…”

Section: Therapeutic Potential Of Stromal Vascular Fractionmentioning

confidence: 99%

Clinical applications of adipose-derived stromal vascular fraction in veterinary practice

Sharun

Jambagi

Kumar

et al. 2022

Veterinary Quarterly

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Adipose tissue-derived stromal vascular fraction (AdSVF) comprises a heterogeneous cell population, including the multipotent mesenchymal stem cells, hematopoietic stem cells, immune cells, endothelial cells, fibroblasts, and pericytes. As such, multipotent adipose tissue-derived mesenchymal stem cells (AdMSCs), are one of the important components of AdSVF. Commonly used techniques to harvest AdSVF involve enzymatic or non-enzymatic methods. The enzymatic method is considered to be the gold standard technique due to its higher yield. The cellular components of AdSVF can be resuspended in normal saline, platelet-rich plasma, or phosphate-buffered saline to produce a ready-to-use solution. Freshly isolated AdSVF has exhibited promising osteogenic and vasculogenic capacity. AdSVF has already been proven to possess therapeutic potential for osteoarthritis management. It is also an attractive therapeutic option for enhancing wound healing. In addition, the combined use of AdSVF and platelet-rich plasma has an additive stimulatory effect in accelerating wound healing and can be considered an alternative to AdMSC treatment. It is also widely used for managing various orthopaedic conditions in clinical settings and has the potential for regenerating bone, cartilage, and tendons. Autologous AdSVF cells are used along with bone substitutes and other biological factors as an alternative to conventional bone grafting techniques owing to their promising osteogenic and vasculogenic capacity. It can also be used for treating osteonecrosis, meniscus tear, chondromalacia, and tendon injuries in veterinary practice. It has several advantages over in vitro expanded AdMSC, including precluding the need for culturing, reduced risk of cell contamination, and cost-effectiveness, making it ideal for clinical use.

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Adipose-Derived Stromal/Stem Cells from Large Animal Models: from Basic to Applied Science

Cited by 25 publications

References 152 publications

Perspective on Stem Cell Therapy in Organ Fibrosis: Animal Models and Human Studies

Perspective on Stem Cell Therapy in Organ Fibrosis: Animal Models and Human Studies

Tissue-Material Integration and Biostimulation Study of Collagen Acellular Matrices

Clinical applications of adipose-derived stromal vascular fraction in veterinary practice

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