Adipose-derived stromal cell secretome reduces TNFα-induced hypertrophy and catabolic markers in primary human articular chondrocytes

Niada, Stefania; Giannasi, Chiara; Stanco, D.; Casati, Sara; Brini, A.T.

doi:10.1016/j.scr.2019.101463

Cited by 37 publications

(55 citation statements)

References 45 publications

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“…Effect on viability, proliferation, and migration: A total of 14 papers investigated the effect of EVs or secretome on cell proliferation, viability, and migration (Table 1) [18,20,21,22,23,24,25,26,27,28,29,30,31,32]. Twelve papers reported that EVs derived from MSCs increased the proliferation and/or the viability of OA chondrocytes or chondrocyte progenitor cells [18,20,21,22,23,25,26,28,29,30,31,32], with a dose dependent effect [18,21,22,26], while two papers [24,27] reported no significant effects on chondrocytes viability. One study compared the effect on chondrocyte proliferation of Exo derived from two different MSCs sources [31], iPS-derived MSCs and SMSCs, finding that Exo from iPS-MSCs had superior effects than SMSC-Exo.…”

Section: Resultsmentioning

confidence: 99%

“…Effect on chondrocyte phenotype: Thirteen papers assessed phenotype maintenance and chondrocyte hypertrophy after the treatment with EVs or secretome [20,21,23,24,25,26,27,28,29,30,32,33,34]. Regarding the maintenance or the induction of chondrocyte phenotype, three studies described the increase of the expression of SOX9 [20,25,30], mediated by EVs.…”

Section: Resultsmentioning

confidence: 99%

“…Regarding the maintenance or the induction of chondrocyte phenotype, three studies described the increase of the expression of SOX9 [20,25,30], mediated by EVs. Six works analyzed chondrocyte hypertrophy status after EVs addition [20,24,28,29,30,32], and four of them showed that the expression of the transcription factor RUNX2 diminished with EVs [20,28,30,32]. Liu et al [28] reported that this reduction was dose dependent.…”

Section: Resultsmentioning

confidence: 99%

“…Liu et al [28] reported that this reduction was dose dependent. At protein level, four papers observed the decreased expression of type-X collagen mediated by EVs, either normal [20,24] or overexpressing miR-92a-3p [30] or miR-95-5p [29]. Similarly, the level of other two hypertrophy markers (alkaline phosphatase and osteocalcin) were lowered [20,24].…”

Section: Resultsmentioning

confidence: 99%

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Secretome and Extracellular Vesicles as New Biological Therapies for Knee Osteoarthritis: A Systematic Review

D’Arrigo

Roffi

Cucchiarini

et al. 2019

JCM

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Secretome and extracellular vesicles (EVs) are considered a promising option to exploit mesenchymal stem cells’ (MSCs) properties to address knee osteoarthritis (OA). The aim of this systematic review was to analyze both the in vitro and in vivo literature, in order to understand the potential of secretome and EVs as a minimally invasive injective biological approach. A systematic review of the literature was performed on PubMed, Embase, and Web of Science databases up to 31 August 2019. Twenty studies were analyzed; nine in vitro, nine in vitro and in vivo, and two in vivo. The analysis showed an increasing interest in this emerging field, with overall positive findings. Promising in vitro results were documented in terms of enhanced cell proliferation, reduction of inflammation, and down-regulation of catabolic pathways while promoting anabolic processes. The positive in vitro findings were confirmed in vivo, with studies showing positive effects on cartilage, subchondral bone, and synovial tissues in both OA and osteochondral models. However, several aspects remain to be clarified, such as the different effects induced by EVs and secretome, which is the most suitable cell source and production protocol, and the identification of patients who may benefit more from this new biological approach for knee OA treatment.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Secretome and Extracellular Vesicles as New Biological Therapies for Knee Osteoarthritis: A Systematic Review

D’Arrigo

Roffi

Cucchiarini

et al. 2019

JCM

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“…Conditioned media were collected from approximately 6 × 10 6 cells in starving conditions, centrifuged for 15 min at 2500× g, at 4 • C to remove debris and large apoptotic bodies, and concentrated through Amicon Ultra-15 Centrifugal Filter Devices with 3 kDa cut-off (Merck Millipore, Milan, Italy) for 90 min at 4000× g, 4 • C [48]. The final product was concentrated about 40-50 folds.…”

Section: Concentrated Conditioned Media (Cm)mentioning

confidence: 99%

Quantitative Lipidomic Analysis of Osteosarcoma Cell-Derived Products by UHPLC-MS/MS

et al. 2020

Self Cite

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Changes in lipid metabolism are involved in several pathological conditions, such as cancer. Among lipids, eicosanoids are potent inflammatory mediators, synthesized from polyunsaturated fatty acids (PUFAs), which coexist with other lipid-derived ones, including endocannabinoids (ECs) and N-acylethanolamides (NAEs). In this work, a bioanalytical assay for 12 PUFAs/eicosanoids and 20 ECs/NAEs in cell culture medium and human biofluids was validated over a linear range of 0.1–2.5 ng/mL. A fast pretreatment method consisting of protein precipitation with acetonitrile followed by a double step liquid–liquid extraction was developed. The final extracts were injected onto a Kinetex ultra-high-performance liquid chromatography (UHPLC) XB-C18 column with a gradient elution of 0.1% formic acid in water and methanol/acetonitrile (5:1; v/v) mobile phase. Chromatographic separation was followed by detection with a triple-quadrupole mass spectrometer operating both in positive and negative ion-mode. A full validation was carried out in a small amount of cell culture medium and then applied to osteosarcoma cell-derived products. To the best of our knowledge, this is the first lipid profiling of bone tumor cell lines (SaOS-2 and MG-63) and their secretome. Our method was also partially validated in other biological matrices, such as serum and urine, ensuring its broad applicability as a powerful tool for lipidomic translational research.

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Fat-Derived Stem Cells

Libonati

Colombini

Orfei

2021

Early Osteoarthritis

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Adipose-derived stromal cell secretome reduces TNFα-induced hypertrophy and catabolic markers in primary human articular chondrocytes

Cited by 37 publications

References 45 publications

Secretome and Extracellular Vesicles as New Biological Therapies for Knee Osteoarthritis: A Systematic Review

Secretome and Extracellular Vesicles as New Biological Therapies for Knee Osteoarthritis: A Systematic Review

Quantitative Lipidomic Analysis of Osteosarcoma Cell-Derived Products by UHPLC-MS/MS

Fat-Derived Stem Cells

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