Adipose-derived stromal cell cluster with light therapy enhance angiogenesis and skin wound healing in mice

Park, In-Su; Chung, Phil‐Sang; Ahn, Jin Ho

doi:10.1016/j.bbrc.2015.04.059

Cited by 40 publications

(49 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The study performed by Cury et al () demonstrated an enhanced VEGF mRNA expression in a dose‐dependent manner after red and infrared irradiation. A detailed study about vascular regeneration after LLLT on spheroid ASC in hind limb ischaemia mice was performed by the group of Park, Chung, and Ahn (, , ), who demonstrated enhanced secretion of angiogenic growth factors and improved functional recovery.…”

Section: Discussionmentioning

confidence: 99%

Photobiomodulation of freshly isolated human adipose tissue-derived stromal vascular fraction cells by pulsed light-emitting diodes for direct clinical application

Priglinger

Maier

Chaudary

et al. 2018

J Tissue Eng Regen Med

View full text Add to dashboard Cite

A highly interesting source for adult stem cells is adipose tissue, from which the stromal vascular fraction (SVF)-a heterogeneous cell population including the adipose-derived stromal/stem cells-can be obtained. To enhance the regenerative potential of freshly isolated SVF cells, low-level light therapy (LLLT) was used. The effects of pulsed blue (475 nm), green (516 nm), and red (635 nm) light from light-emitting diodes applied on freshly isolated SVF were analysed regarding cell phenotype, cell number, viability, adenosine triphosphate content, cytotoxicity, and proliferation but also osteogenic, adipogenic, and proangiogenic differentiation potential. The colony-forming unit fibroblast assay revealed a significantly increased colony size after LLLT with red light compared with untreated cells, whereas the frequency of colony-forming cells was not affected. LLLT with green and red light resulted in a stronger capacity to form vascular tubes by SVF when cultured within 3D fibrin matrices compared with untreated cells, which was corroborated by increased number and length of the single tubes and a significantly higher concentration of vascular endothelial growth factor. Our study showed beneficial effects after LLLT on the vascularization potential and proliferation capacity of SVF cells. Therefore, LLLT using pulsed light-emitting diode light might represent a new approach for activation of freshly isolated SVF cells for direct clinical application.

show abstract

Section: Discussionmentioning

confidence: 99%

Photobiomodulation of freshly isolated human adipose tissue-derived stromal vascular fraction cells by pulsed light-emitting diodes for direct clinical application

Priglinger

Maier

Chaudary

et al. 2018

J Tissue Eng Regen Med

View full text Add to dashboard Cite

show abstract

“…In these cases, the laser is employed as a therapy through low-intensity laser therapy (LLLT) [30] and is used to induce analgesia, anti-inflammation, and biomodulation [30,31]. The primary effects of LLLT occur at the cellular and/or molecular level, later generating secondary effects, such as increased cellular metabolism, collagen synthesis, DNA and RNA modifications, local effects on the immune system, and increased angiogenesis [32,33]. These properties suggest that LLLT could minimize the damage to the pulp resulting from oxidative stress stemming from the bleaching procedure [29].…”

Section: Introductionmentioning

confidence: 99%

Influence of different types of light on the response of the pulp tissue in dental bleaching: a systematic review

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The deposition of ECM such as collagen III provides a scaffold for the migration of fibroblasts and the elongation of blood vessels 51,52 . The regeneration of new blood vessels provides nutrients to fibroblasts 10 . As the main cell type of skin tissue, proliferation and migration of fibroblasts in ECM is the key factor promoting the formation of granulation tissue 5 , which in turn secretes more ECM and growth factors that promote angiogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…Infusion of human ASCs promoted repair of diabetic foot ulcers in rats 26 . ASCs in combination with light therapy enhanced angiogenesis and skin wound healing in mice 10 . Human ASCs seeded on a silk fibroin-chitosan scaffold enhanced wound repair by improving neovascularization in a murine soft tissue injury model 27 .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Adipose Stem Cells from Type 2 Diabetic Mice Exhibit Therapeutic Potential in Wound Healing

Sun

Song

Zhang

et al. 2020

Preprint

View full text Add to dashboard Cite

BACKGROUND: Diabetic patients suffer from impaired wound healing. Mesenchymal stem cell (MSC) therapy represents a promising approach toward improving skin wound healing through release of soluble growth factors and cytokines that stimulate new vessel formation and modulate inflammation. Whether adipose-derived MSCs (ASCs) from type 2 diabetes donors are suitable for skin damage repair remains largely unknown. METHODS: In this study, we compared the phenotype and functionality of ASCs harvested from high fat diet (HFD) and streptozotocin (STZ)-induced T2D or control mice, and assessed their abilities to promote wound healing in an excisional wound splinting mouse model with T2D. RESULTS: T2D ASCs expressed similar cellular markers as control ASCs, but secreted less hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), and transforming growth factor β (TGF-β). T2D ASCs were somewhat less effective in promoting healing of the wound, as manifested by slightly reduced re-epithelialization, cutaneous appendage regeneration, and collagen III deposition in wound tissues. In vitro, T2D ASCs promoted proliferation and migration of skin fibroblasts to a comparable extent as control ASCs via suppression of inflammation and macrophage infiltration. CONCLUSIONS: From these findings, we conclude that, although ASCs from T2D mice are marginally inferior to control ASCs, they possess comparable therapeutic effects in wound healing.

show abstract

Adipose-derived stromal cell cluster with light therapy enhance angiogenesis and skin wound healing in mice

Cited by 40 publications

References 28 publications

Photobiomodulation of freshly isolated human adipose tissue-derived stromal vascular fraction cells by pulsed light-emitting diodes for direct clinical application

Photobiomodulation of freshly isolated human adipose tissue-derived stromal vascular fraction cells by pulsed light-emitting diodes for direct clinical application

Influence of different types of light on the response of the pulp tissue in dental bleaching: a systematic review

Adipose Stem Cells from Type 2 Diabetic Mice Exhibit Therapeutic Potential in Wound Healing

Contact Info

Product

Resources

About