Adipose-derived stem/progenitor cells from lipoaspirates: A comparison between the Lipivage200-5 liposuction system and the Body-Jet liposuction system

Taha, Sara; Saller, Maximilian Michael; Haas, E.; Farkas, Zsuzsanna; Aszódi, Attila; Giunta, Riccardo E.; Volkmer, Elias

doi:10.1016/j.bjps.2019.06.025

Cited by 11 publications

(12 citation statements)

References 32 publications

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“…The osteogenic differentiation was performed essentially as previously described 15,16 . Briefly, cells were expanded in a 6‐well plate for confluency and the differentiation was induced by changing to an osteogenic induction medium composed of CM supplemented with 100 nmol/L dexamethasone, 10 mmol/L β‐glycerolphosphate, and 50 μmol/L ascorbic acid (all from Sigma‐Aldrich).…”

Section: Methodsmentioning

confidence: 99%

Experimental approach to nasal septal cartilage regeneration with adipose tissue‐derived stem cells and decellularized porcine septal cartilage

Kuhlmann¹,

Schenck²,

Tluczynski

et al. 2020

Xenotransplantation

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Background Cartilage shortage is a major problem in facial reconstructive surgery. Prior studies have shown that decellularized porcine nasal septal cartilage (DPNC) seeded with primary human nasal chondrocytes enabled cartilage regeneration and showed potential as a replacement material for nasal cartilage. Since adipose tissue‐derived stem cells (ASCs) are easily accessible and almost abundantly available, they appear to be a promising alternative to limited chondrocytes making the combination of DPNC and ASCs a feasible approach towards clinical translation. Thus, this study was intended to investigate the interactions between ASCs and DPNC in an in vitro model. Methods DPNCs were seeded and 3D‐cultured with primary human ASCs that were priorly characterized with trilineage differentiation and flow cytometry. Cell vitality and proliferation were evaluated by Live‐Dead, alamarBlue, and PicoGreen assays. Chondrogenic differentiation was examined by DMMB assay and cryosectioning‐based histology. Cell invasion within DPNC was visualized and quantified by fluorescent histology (DAPI, Phalloidin). Results ASCs showed good adherence to DPNC and Live‐Dead assay proved their viability over 2 weeks. AlamarBlueassay showed an increase in metabolic activity compared to 2D cultures, and PicoGreen assay demonstrated an increase of cell number within DPNC over time. Biochemical assays and histology added evidence of chondrogenic differentiation of 3D‐cultured ASCs under the influence of chondrogenic induction medium. Fluorescent image analysis showed a significant increase of cell‐occupied areas of scaffolds over time (P < .05). Conclusions DPNC scaffolds provided a suitable environment for ASCs that allowed good cell vitality, high proliferation, and chondrogenic differentiation. Thus, the use of ASCs and DPNC yields a promising alternative to the use of primary human chondrocytes. For facial cartilage tissue engineering, we regard ASCs as an attractive alternative to human nasal chondrocytes due to their better accessibility and availability. Further research will be necessary to determine long‐term effects and in vivo outcomes of ASCs and DPNC in cartilage regeneration of the face.

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Section: Methodsmentioning

confidence: 99%

Experimental approach to nasal septal cartilage regeneration with adipose tissue‐derived stem cells and decellularized porcine septal cartilage

Kuhlmann¹,

Schenck²,

Tluczynski

et al. 2020

Xenotransplantation

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“…The increased percentage of CD146 + cells may be explained by the fact that the water jet procedure is less traumatic and the pericytes are better preserved [15] . Another study also reported a tendency for stronger adipogenic potential of cells isolated with water jet-assisted liposuction versus cells obtained by suction-assisted liposuction [12] . We have also described that CD146 + ASCs have enhanced angiogenic potential [10] , which was confirmed by another recent study that showed the benefits of supplementing fat grafts with CD146-selected cells [16] .…”

Section: Discussionmentioning

confidence: 89%

“…Earlier studies have shown a variance of 10% −80% survival of fat grafts that are not augmented with ASCs. The water jet technique has shown promising results with regard to cell viability [12][13][14] and when used in the absence of cell supplementation, it is possible to achieve better fat survival using the water jet technique when compared with manual isolation [14] .…”

Section: Discussionmentioning

confidence: 99%

Water jet-assisted lipoaspiration and Sepax cell separation system for the isolation of adipose stem cells with high adipogenic potential

Lauvrud¹,

Gümüscü²,

Wiberg³

et al. 2021

Journal of Plastic, Reconstructive & Aesthetic Surgery

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Water jet-assisted liposuction has gained popularity due to favourable fat grafting outcomes. In this study, we compared stem cells obtained from fat isolated with manual or the water jet-assisted procedure. Methods: Liposuction of abdominal fat was performed using the two methods on each donor ( n = 10). Aspirate samples were collagenase digested and the isolated cells seeded in vitro prior to proliferation, adipogenic differentiation and angiogenic activity analyses. Results: Cells from either procedure proliferated at similar rates and exhibited a similar colony-forming ability. The cells expressed stem cell markers CD73, CD90 and CD105. In the water jet cell preparations, there were higher numbers of cells expressing CD146. Robust adipogenic differentiation was observed in cultures expanded from both manual and water jet lipoaspirates. Gene analysis showed higher expression of the adipocyte markers aP2 and GLUT4 in the adipocyte-differentiated water jet cell preparations, and ELISA indicated increased secretion of adiponectin from these cells. Both cell groups expressed vasculogenic factors and the water jet cells promoted the highest levels of in vitr o angiogenesis. Given these positive results, we further characterised the water jet cells when prepared using an automated closed Parts of this work were presented at the International Federation for Adipose Therapeutics and Science (IFATS) annual meetings held in 2016 and 2018.

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“…ADSCs were isolated from approximately 10 g of the same L-WAT with a semi-automated centrifuge system (ARC™-Processing Unit, InGeneron, USA), following the manufacturer's protocol and using its enzyme blend (Matrase™) and 37 • C warm lactated Ringer's solution (Fresenius Kabi, Germany). Stem cell properties were proven by differentiating a proportion of isolated cells into the osteogenic, adipogenic and chondrogenic lineage, as previously published [67]. For RNA-Seq experiments, freshly isolated cells were expanded for 3 days in standard culture medium, as described above.…”

Section: Preparation Of White Adipose Tissue Cell Isolation and Cultmentioning

confidence: 99%

Differences in the Inflammatory Response of White Adipose Tissue and Adipose-Derived Stem Cells

Taha

Volkmer

Haas

et al. 2020

IJMS

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The application of liposuctioned white adipose tissue (L-WAT) and adipose-derived stem cells (ADSCs) as a novel immunomodulatory treatment option is the currently subject of various clinical trials. Because it is crucial to understand the underlying therapeutic mechanisms, the latest studies focused on the immunomodulatory functions of L-WAT or ADSCs. However, studies that examine the specific transcriptional adaptation of these treatment options to an extrinsic inflammatory stimulus in an unbiased manner are scarce. The aim of this study was to compare the gene expression profile of L-WAT and ADSCs, when subjected to tumor necrosis factor alpha (TNFα), and to identify key factors that might be therapeutically relevant when using L-WAT or ADSCs as an immuno-modulator. Fat tissue was harvested by liposuction from five human donors. ADSCs were isolated from the same donors and shortly subjected to expansion culture. L-WAT and ADSCs were treated with human recombinant TNFα, to trigger a strong inflammatory response. Subsequently, an mRNA deep next-generation sequencing was performed to evaluate the different inflammatory responses of L-WAT and ADSCs. We found significant gene expression changes in both experimental groups after TNFα incubation. However, ADSCs showed a more homogenous gene expression profile by predominantly expressing genes involved in immunomodulatory processes such as CCL19, CCL5, TNFSF15 and IL1b when compared to L-WAT, which reacted rather heterogeneously. As RNA sequencing between L-WAT and ADSCS treated with TNFα revealed that L-WAT responded very heterogeneously to TNFα treatment, we therefore conclude that ADSCs are more reliable and predictable when used therapeutically. Our study furthermore yields insight into potential biological processes regarding immune system response, inflammatory response, and cell activation. Our results can help to better understand the different immunomodulatory effects of L-WAT and ADSCs.

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Adipose-derived stem/progenitor cells from lipoaspirates: A comparison between the Lipivage200-5 liposuction system and the Body-Jet liposuction system

Cited by 11 publications

References 32 publications

Experimental approach to nasal septal cartilage regeneration with adipose tissue‐derived stem cells and decellularized porcine septal cartilage

Experimental approach to nasal septal cartilage regeneration with adipose tissue‐derived stem cells and decellularized porcine septal cartilage

Water jet-assisted lipoaspiration and Sepax cell separation system for the isolation of adipose stem cells with high adipogenic potential

Differences in the Inflammatory Response of White Adipose Tissue and Adipose-Derived Stem Cells

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