Adipose-Derived Stem Cells Promote Proliferation, Migration, and Tube Formation of Lymphatic Endothelial Cells In Vitro by Secreting Lymphangiogenic Factors

Takeda, Kazuhisa; Sowa, Yoshihiro; Nishino, Koichi; Itoh, Kyoko; Fushiki, Shinji

doi:10.1097/sap.0000000000000084

Cited by 60 publications

(58 citation statements)

References 56 publications

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“…Indeed, these cells have been shown to promote adipose cell replication, incorporate into vessel walls, and decrease the local inflammatory response (Rehman et al 2004). Potentially, they produce a series of autocrine factors, that affect the cell itself, and paracrine, that have an effect on neighboring cells (Yang et al 2010;Takeda et al 2015;Wang et al 2015). Moreover, they may contribute to neo-angiogenesis in the acute phase by secreting angiogenic factors and by acting as endothelial progenitor cells (Tiryaki et al 2011).…”

Section: Discussionmentioning

confidence: 99%

“…These are pluripotent mesenchymal stem cells that are similar to bone marrow-derived mesenchymal stem cells (Sterodimas et al 2011;Tiryaki et al 2011;Park et al 2008). Indeed, ADSCs are able to differentiate in mature adipose cells or other adult mesenchymal cells after paracrine or autocrine hormonal stimulations (Yang et al 2010;Takeda et al 2015;Wang et al 2015;Salibian et al 2013). This characteristic of ADSCs would allow the regeneration and, therefore, a longer survival of the implanted tissues (Ryu et al 2013;Ellenbogen et al 2005).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of adipose-derived stem cells freshly isolated from liposuction aspirates performed with Prolipostem®

Savoia¹,

Perna

Pasquale³

et al. 2017

biomed dermatol

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Background: Lipofilling is a cosmetic surgical procedure that consists in the withdrawal of a small quantity of fat tissue from a suitable anatomic area and the reimplantation of this tissue in another corporeal district in the same individual, so as to obtain a filling effect. Recently, adipose-derived stem cells (ADSCs) have been isolated in the aspirates. These are pluripotent mesenchymal stem cells that are able to differentiate in mature adipose cells or other adult mesenchymal cells after paracrine or autocrine hormonal stimulations, thus favoring a longer survival of the implanted tissues. Methods: In this article, we have defined a new method for liposuction (Prolipostem®), where the ADSCs are recovered and mixed with the suctioned adipose tissue derived from the abdominal fat, before the reimplantation. Results: We have demonstrated by immunocytochemistry the presence of ADSCs in the adipose tissue taken with Prolipostem® from the abdominal fat and the ability of these ADSCs to differentiate in mature adipose cells in vitro. Conclusions: The possibility to enrich the tissue to be implanted with ADSCs would assure a longer survival of the cells implanted and a regeneration of the host tissue thanks to growth and angiogenic stimuli induced by the ADSCs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of adipose-derived stem cells freshly isolated from liposuction aspirates performed with Prolipostem®

Savoia¹,

Perna

Pasquale³

et al. 2017

biomed dermatol

View full text Add to dashboard Cite

show abstract

“…Matrigel tube formation assays were carried out as previously described [13]. Briefly, matrigel (BD Biosciences, Bedford, MA, USA) was placed in 24-well plates (100mL/well) and allowed to gel at 37 o C for 30 minutes.…”

Section: Tube Formation Assaymentioning

confidence: 99%

“…Vascular endothelial growth factor-C (VEGF-C) is the first powerful lymphangiogenic factor [13,14]. Lymphangiogenesis is mediated by binding of VEGF-C to VEGFR-3.…”

Section: Introductionmentioning

confidence: 99%

Exosomes from Adipose-Derived Stem Cells Promotes VEGF-C-Dependent Lymphangiogenesis by Regulating miRNA-132/TGF-β Pathway

Wang

Cao

et al. 2018

Cell Physiol Biochem

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Background/Aims: Lymphangiogenesis plays an important role in the pathogenesis of inflammatory bowel diseases (IBD), and vascular endothelial growth factor-C (VEGF-C) is a powerful lymphangiogenic factor. Adipose-derived stem cells (ADSCs) are a promising therapeutic modality for several diseases because ADSCs secret growth factors and exosomes, which modulate hostile microenvironments affected by diseases. However, the effect of exosomes on VEGF-C-dependent lymphangiogenesis and its mechanism remain unclear. Methods: ADSCs were cultured in media with or without recombinant VEGF-C and exosomes were extracted from conditioned medium (CM). Lymphatic endothelial cells (LECs) were treated with ADSCs-derived exosomes, then proliferation, migration and tube formation of LECs were assayed using cell counting Kit-8 (CCK-8), transwell chamber inserts and matrigel-based tube formation assay respectively. Results: We identified significantly higher levels of miR-132 in exosomes isolated from VEGF-C-treated ADSCs (ADSCs/VEGF-C) than in those from ADSCs control. miR-132 was directly transferred from ADSCs to the LECs by the mediation of exosomes. The exosomes from ADSCs/VEGF-C promoted LECs proliferation, migration, and tube formation more potently than the exosomes from ADSCs, whereas pretreatment of ADSCs with miR-132 inhibitor attenuates VEGF-C-dependent lymphangiogenic response. Finally we reveal that miR-132 promotes lymphangiogenic response by directly targeting Smad-7 and regulating TGF-β/Smad signaling. Conclusion: These data provide new insights into the role of ADSCs-derived exosomes as an important player in VEGF-C-dependent lymphangiogenesis.

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“…Moreover, stem cells exhibit immunomodulatory abilities affecting the length and strength of the inflammatory response, tissue remodeling, and thus the overall structure of the local extracellular matrix (ECM) and surrounding microenvironment [23,24]. Furthermore, adipose tissue-derived stem cells (ADSCs) have been found to secrete lymphangiogenic factors and promote the formation of the lymphatic system [25], exhibiting an even more striking effect on the immune system. …”

Section: Introductionmentioning

confidence: 99%

Stem Cells in Functional Bladder Engineering

Smolar

Salemi

Horst

et al. 2016

Transfus Med Hemother

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Conditions impairing bladder function in children and adults, such as myelomeningocele, posterior urethral valves, bladder exstrophy or spinal cord injury, often need urinary diversion or augmentation cystoplasty as when untreated they may cause severe bladder dysfunction and kidney failure. Currently, the gold standard therapy of end-stage bladder disease refractory to conservative management is enterocystoplasty, a surgical enlargement of the bladder with intestinal tissue. Despite providing functional improvement, enterocystoplasty is associated with significant long-term complications, such as recurrent urinary tract infections, metabolic abnormalities, stone formation, and malignancies. Therefore, there is a strong clinical need for alternative therapies for these reconstructive procedures, of which stem cell-based tissue engineering (TE) is considered to be the most promising future strategy. This review is focused on the recent progress in bladder stem cell research and therapy and the challenges that remain for the development of a functional bladder wall.

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Adipose-Derived Stem Cells Promote Proliferation, Migration, and Tube Formation of Lymphatic Endothelial Cells In Vitro by Secreting Lymphangiogenic Factors

Cited by 60 publications

References 56 publications

Characterization of adipose-derived stem cells freshly isolated from liposuction aspirates performed with Prolipostem®

Characterization of adipose-derived stem cells freshly isolated from liposuction aspirates performed with Prolipostem®

Exosomes from Adipose-Derived Stem Cells Promotes VEGF-C-Dependent Lymphangiogenesis by Regulating miRNA-132/TGF-β Pathway

Stem Cells in Functional Bladder Engineering

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