Adipose-derived stem cells from pregnant women show higher proliferation rate unrelated to estrogen

Ng, Lui; Yip, S. K. H.; Wong, Hei-Kiu; Yam, Gary Hin Fai; Liu, Y.M.; Lui, Wai Ting; Wang, Chi Chiu; Choy, Kwong Wai

doi:10.1093/humrep/dep001

Cited by 20 publications

(27 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to their findings, whereas male ADSCs isolated from superficial adipose layer have greater osteogenic capacity than deep layer cells, no significant difference was observed between the cultures isolated from female donors. In contrast to our and previous results, Ng et al (2009) found that both donor reproductive status (pregnancy, premenopause and postmenopause) and in vitro E 2 treatment have no significant effect on proliferation rate of human ADSCs. Overall, these conflicting findings may reflect the fact that proliferation rate and differentiation potential of stem cells including ADSCs can be influenced by several factors, such as donor age, gender, cell isolation and culture techniques, tissue sources.…”

Section: Discussioncontrasting

confidence: 99%

Stimulatory effect of 17β-estradiol on osteogenic differentiation potential of rat adipose tissue-derived stem cells

Taşkıran

Evren²

2011

gpb

View full text Add to dashboard Cite

Abstract. Adipose tissue-derived stem cells (ADSCs) are considered as a potential cell source for regenerative medicine and tissue engineering. Although ADSCs have greater proliferation capacity than bone marrow stem cells (BMSCs), lower differentiation ability of these cells limits their utility in experimental and clinical studies. The purpose of this study was to investigate whether 17β-estradiol (E 2 ) has a stimulatory effect on osteogenic differentiation potential of ADSCs in vitro. ADSCs were isolated from visceral adipose tissues of rats and treated with different concentrations of E 2 in osteogenic medium (OM) for 21 days. The differences in osteogenic differentiation potential of the cultures were assessed by von Kossa staining, measurement of alkaline phosphatase (ALP) activity and calcium levels. ADSCs cultured in OM supplemented with E 2 showed greater bone-like nodule formation and mineral deposition in comparing with the cells grown in OM. In addition, ALP activity and calcium levels also were significantly higher in the cultures exposed to E 2 than the cells treated only with OM (p < 0.005, n = 5). Our results suggest that E 2 may stimulate the osteogenic differentiation of ADSCs and therefore, can be used as an inducing agent to improve the efficiency of these cells in in vitro and in vivo studies.

show abstract

Section: Discussioncontrasting

confidence: 99%

Stimulatory effect of 17β-estradiol on osteogenic differentiation potential of rat adipose tissue-derived stem cells

Taşkıran

Evren²

2011

gpb

View full text Add to dashboard Cite

show abstract

“…Therefore, we hypothesize that the main effects of estrogen in improving ASC proliferation, migration and adipogenesis is mediated by ERα. In contrast to this, it was published that estrogen level variation can hardly alter the capacity for stem cell proliferation [36]. Differences between results of Ng et al and ours might result because different concentrations of estrogen were used for ASC-based experiments [36].…”

Section: Discussioncontrasting

confidence: 57%

Estrogen Receptor α and β in Mouse: Adipose-Derived Stem Cell Proliferation, Migration, and Brown Adipogenesis In Vitro

Zhang

Schmull

et al. 2016

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Adipose-derived stem cells (ASCs) belong to mesenchymal stem cells and may play a potential role as seeding cells in stem cell transplantation. To be able to exploit stem cells as therapeutic tool, their defects in some important cellular functions, such as low survival rate and cellular activity, should be considered. This is especially the case for stem cells that are intended for transplantation. Of note, stem cell responses to hormones should be considered since estrogen is known to play a critical role in stem cell behavior. However, different impacts of the estrogen receptor (ER) types α and β have not been fully determined in ASC function. In this study, we investigated effects of ERα and ERβ on ASC proliferation, migration, as well as in adipogenesis. Methods: ASCs obtained from mice were cultured with 100nM ERα or ERβ agonist PPT and DPN, respectively. The ERα and ERβ antagonist ICI 182,780 (100nM) was used as control. Results: Compared to ERβ, ERα appears more potent in improving ASC proliferation and migration. Investigation of adipogenesis revealed that ERβ played a significant role in suppressing ASC-mediated brown tissue adipogenesis which is in contrast to ERα. These results correlated with reduced mRNA expression of UCP-1, PGC-1α and PPAR-γ. Conclusions: ERα plays a more critical role in promoting ASC proliferation and migration while ERβ is more potent in suppressing ASC brown adipose tissue differentiation mediated by decreased UCP-1, PGC-1α and PPAR-γ expression.

show abstract

“…Manotaya et al (2002) indicated that hematopoietic stem cell number and activity increased during pregnancy. In addition, Ng et al (2009) found that the proliferation rate of ADSCs from pregnant women was higher than ADSCs isolated from preand postmenopausal women. We also detected the effect of progesterone and estradiol on rADSC proliferation.…”

Section: Discussionmentioning

confidence: 99%

“…The estrogen receptor is the decisive factor influencing cellular response to estrogen (Cobellis et al, 2002), and it was shown to exist in MSCs (Zhou et al, 2001;Wang et al, 2006). Ng et al (2009) showed that upon binding estrogen receptors on the cell surface, estrogen activated specific genes and eventually affected cellular processes. However, no differences in proliferation rates were observed when ADSCs from pregnant, premenopausal or menopausal women were treated with exogenous estrogen.…”

Section: Discussionmentioning

confidence: 99%

Effect of pregnancy on the proliferation of rat adipose-derived stem cells

Zhang

Wang

et al. 2017

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Stem cell therapy faces many problems including poor survival rates and low viability. Enhancing the biological functions of stem cells improves efficacy of therapies. Estrogen, whose levels are elevated during pregnancy, affects the properties of bone marrow mesenchymal stem cells. Evidence suggests that adipose-derived stem cells (ADSCs), which are a type of adult mesenchymal stem cells, can be used in regenerative medicine. In fact, ADSCs from pregnant animals have been used in clinical therapies. However, the effect of the donor's reproductive status on proliferation of ADSCs is unknown. We investigated the effect of 17b-estradiol (E2) and progesterone (P) on the in vitro proliferation of ADSCs from laboratory rats. ADSCs were obtained from five different groups of 15 rats each -non-pregnant, pregnant, in perinatal period, non-pregnant and treated with E2, and non-pregnant and treated with P. Adhesion and viability of ADSCs were determined by MTT assay, and cell cycle was followed by flow cytometry. The proliferation rate of ADSCs from pregnant rats was significantly higher than those from the non-pregnant rats (P < 0.05); however, there was no statistically significant difference in proliferation rates during different phases of pregnancy (P > 0.05). Additionally, ADSCs from pregnant rats possess higher adhesion property in early stage (P1 passage) and higher proliferation rate than ADSCs from nonpregnant rats. Interestingly, ADSCs from non-pregnant rats that were treated with E2, but not those treated with P, showed higher proliferation rates than those from their untreated counterparts. These results suggest that the proliferative capacity and residence time in different cell cycle phases of ADSCs can be regulated by extrinsic factors such as estrogen concentration.

show abstract

Adipose-derived stem cells from pregnant women show higher proliferation rate unrelated to estrogen

Abstract: Despite the higher proliferation rate in pregnant women, ASCs showed consistency in cell differentiation capacity and were unaffected by donor status. This suggests that factors other than estrogen are responsible for the difference in proliferation.

Cited by 20 publications

References 14 publications

Stimulatory effect of 17β-estradiol on osteogenic differentiation potential of rat adipose tissue-derived stem cells

Stimulatory effect of 17β-estradiol on osteogenic differentiation potential of rat adipose tissue-derived stem cells

Estrogen Receptor α and β in Mouse: Adipose-Derived Stem Cell Proliferation, Migration, and Brown Adipogenesis In Vitro

Effect of pregnancy on the proliferation of rat adipose-derived stem cells

Contact Info

Product

Resources

About