Adipose-Derived Stem Cells and Their Potential to Differentiate into the Epithelial Lineage

Baer, Patrick C.

doi:10.1089/scd.2011.0086

Cited by 78 publications

(63 citation statements)

References 126 publications

(186 reference statements)

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“…The properties of ADSCs are similar to BMSCs in that they also have the capacity to differentiate into various kinds of cells [89][90][91][92]. Because humans have abundant sources for ADSCs (i.e., subcutaneous fat deposits that can be isolated by conventional liposuction procedures), they are also expected to be a practical cell source for cellbased therapy.…”

Section: • Adscsmentioning

confidence: 99%

Mesenchymal Stem Cells and Umbilical Cord as Sources for Schwann Cell Differentiation: their Potential in Peripheral Nerve Repair

Kuroda¹

2011

TOTERMJ

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Schwann cells are important components of the peripheral glia that form myelin, serving as the microenvironment of nerve fibers in the peripheral nervous system (PNS). Damage to the PNS induces the differentiation and activation of Schwann cells to produce factors that strongly promote axonal regrowth, and subsequently contribute to remyelination, which is crucial for the recovery of function. Although the collection and transplantation of native Schwann cells are effective for the treatment of neural diseases, isolation of Schwann cells results in new damage to other peripheral nerve segments and causes undesirable iatrogenic injury in the donor. Furthermore, the expansion of native Schwann cells to obtain a sufficient number of cells for clinical application within a reasonable period is technically difficult. Therefore, a method to induce easily accessible and highly proliferative cells to differentiate into cells with Schwann cell properties would be very practical and is highly desirable. Recently, regenerative medicine has focused on mesenchymal stem cells because they are easily accessible from various kinds of mesenchymal tissues such as the umbilical cord, bone marrow, and fat tissue. Mesenchymal stem cells are highly proliferative and it is easy to obtain an adequate number of cells. Notably, while mesenchymal stem cells are mesodermal lineage cells, they have an ability to cross oligolineage boundaries previously thought uncrossable to achieve transdifferentiation. In this review, we focus on the potential of mesenchymal stem cells, particularly umbilical cord-derived mesenchymal stem cells, to differentiate into functional Schwann cells, and discuss the prospective clinical application of these cells to PNS regeneration.

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Section: • Adscsmentioning

confidence: 99%

Mesenchymal Stem Cells and Umbilical Cord as Sources for Schwann Cell Differentiation: their Potential in Peripheral Nerve Repair

Kuroda¹

2011

TOTERMJ

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“…Bone marrow aspirates yield an average of 6 × 10 6 nucleated cells per ml, and MSC only represent 0.001 to 0.01% of all the nucleated cells. After considering these drawbacks, ADSC is suggested as a less obtrusive source to obtain multipotent stem cells 25,26 .…”

Section: Discussionmentioning

confidence: 99%

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Chen

Liu

Chang

et al. 2016

JoVE

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Obesity is an unconstrained worldwide epidemic. Unraveling molecular controls in adipose tissue development holds promise to treat obesity or diabetes. Although numerous immortalized adipogenic cell lines have been established, adipose-derived stem cells from the stromal vascular fraction of subcutaneous white adipose tissues provide a reliable cellular system ex vivo much closer to adipose development in vivo. Pig adipose-derived stem cells (pADSC) are isolated from 7-to 9-day old piglets. The dorsal white fat depot of porcine subcutaneous adipose tissues is sliced, minced and collagenase digested. These pADSC exhibit strong potential to differentiate into adipocytes. Moreover, the pADSC also possess multipotency, assessed by selective stem cell markers, to differentiate into various mesenchymal cell types including adipocytes, osteocytes, and chondrocytes. These pADSC can be used for clarification of molecular switches in regulating classical adipocyte differentiation or in direction to other mesenchymal cell types of mesodermal origin. Furthermore, extended lineages into cells of ectodermal and endodermal origin have recently been achieved. Therefore, pADSC derived in this protocol provide an abundant and assessable source of adult mesenchymal stem cells with full multipotency for studying adipose development and application to tissue engineering of regenerative medicine.

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“…ASCs have shown a high proliferative potential and multidifferentiation potential into epithelial lineage 14,15,29 besides myogenic, 30,31 osteogenic, 18,32 , and chondrogenic lineages. 33,34 And the harvesting of ASCs would cause less trauma to donor site compared with autologous epithelium.…”

Section: Figmentioning

confidence: 99%

253 Epithelial-Differentiated Adipose-Derived Stem Cells Seeded Bladder Acellular Matrix Grafts for Urethral Reconstruction: An Animal Model

Li¹,

Xu²,

Fei³

et al. 2013

Journal of Urology

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The limited amount of available epithelial tissue is considered a main cause of the high rate of urethral reconstruction failures. The aim of this study was to investigate whether epithelial-differentiated rabbit adiposederived stem cells (Epith-rASCs) could play a role of epithelium in vivo functionally and be a potential substitute of urothelium. Substitution urethroplasty was performed to repair an anterior urethral defect in male New Zealand rabbits using Epith-rASCs seeded bladder acellular matrix grafts (BAMGs) after 5-bromo-2¢-deoxyuridine (BrdU) labeling, based on the in vitro epithelial induction system we previously described. Urethroplasty with cell-free BAMGs and with undifferentiated rASCs (Und-rASCs) seeded BAMGs were performed as controls. After surgery, a notable amelioration of graft contracture and recovery of urethral continuity were observed in the Epith-rASCs/BAMG group by retrograde urethrograms and macroscopic inspection. Immunofluorescence revealed that the BrdU-labeled Epith-rASCs/Und-rASCs colocalized with cytokeratin 13 or myosin. Consistent with the results of western blotting, at early postimplantation stage, the continuous epithelial layer with local multilayered structure was observed in the Epith-rASCs/BAMG group, whereas no significant growth and local monolayer growth profile of epithelial cells were observed in the BAMG and Und-rASCs/BAMG group, respectively. The results showed that Epith-rASCs could serve as a potential substitute of urothelium for urethral tissue engineering and be available to prevent lumen contracture and subsequent complications including recurrent stricture.

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Adipose-Derived Stem Cells and Their Potential to Differentiate into the Epithelial Lineage

Cited by 78 publications

References 126 publications

Mesenchymal Stem Cells and Umbilical Cord as Sources for Schwann Cell Differentiation: their Potential in Peripheral Nerve Repair

Mesenchymal Stem Cells and Umbilical Cord as Sources for Schwann Cell Differentiation: their Potential in Peripheral Nerve Repair

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

253 Epithelial-Differentiated Adipose-Derived Stem Cells Seeded Bladder Acellular Matrix Grafts for Urethral Reconstruction: An Animal Model

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