Adipose-derived stem cell sheets functionalized by hybrid baculovirus for prolonged GDNF expression and improved nerve regeneration

Hsu, Mu‐Nung; Liao, Han-Tsung; Li, Kuei-Chang; Chen, Hwei-Hsien; Yen, Tzu‐Chen; Макаревич, П.И.; Parfyonova, Y.; Hu, Yu‐Chen

doi:10.1016/j.biomaterials.2017.05.004

Cited by 45 publications

(38 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, seed cells producing neurotrophic factors were widely used to overcome these problems ( Hsueh et al, 2014 ; Zhu et al, 2014 ; Hsu et al, 2017 ). Bone marrow mesenchymal stem cells (BMSCs) are most promising seed cells for nerve repair and regeneration ( Gao et al, 2016 ; Xue et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Brain Derived Neurotrophic Factor and Glial Cell Line-Derived Neurotrophic Factor-Transfected Bone Mesenchymal Stem Cells for the Repair of Periphery Nerve Injury

Zhang¹,

Wu²,

Chen³

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Peripheral nerve injury is a common clinical neurological disease. In our previous study, highly oriented poly ( L -lactic acid) (PLLA)/soy protein isolate (SPI) nanofiber nerve conduits were constructed and exhibited a certain repair capacity for peripheral nerve injury. In order to further improve their nerve repairing efficiency, the bone mesenchymal stem cells (BMSCs) overexpressing brain derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) were introduced into the conduits as seed cells and then were used to repair the 10-mm sciatic nerve defects in rats. The nerve repair efficiency of the functional nerve conduits was evaluated by gait experiment, electrophysiological test, and a series of assays such as hemotoxylin-eosin (HE) staining, immunofluorescence staining, toluidine blue (TB) staining, transmission electron microscopy (TEM) observation of regenerated nerve and Masson’s trichrome staining of gastrocnemius muscle. The results showed that the conduits containing BMSCs overexpressing BDNF and GDNF double-factors group had better nerve repairing efficiency than blank BMSCs and single BDNF or GDNF factor groups, and superior to autografts group in some aspects. These data demonstrated that BDNF and GDNF produced by BMSCs could synergistically promote peripheral nerve repair. This study shed a new light on the conduits and stem cells-based peripheral nerve repair.

show abstract

Section: Introductionmentioning

confidence: 99%

Brain Derived Neurotrophic Factor and Glial Cell Line-Derived Neurotrophic Factor-Transfected Bone Mesenchymal Stem Cells for the Repair of Periphery Nerve Injury

Zhang¹,

Wu²,

Chen³

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…In addition, ASCs survive longer and do not undergo senescence in culture as rapidly as bone marrow-derived stem cells, which allows for greater procedural flexibility. These characteristics suggest that ASCs are well suited to regenerative medicine [ 35 – 38 ], but the best source of ASCs has not yet been elucidated. Our results here suggest that breast may be better than thigh or abdomen as a source of ASCs for regenerative therapy.…”

Section: Discussionmentioning

confidence: 99%

FGF2-induced PI3K/Akt signaling evokes greater proliferation and adipogenic differentiation of human adipose stem cells from breast than from abdomen or thigh

Rong²,

Liang

et al. 2020

Aging

View full text Add to dashboard Cite

In this study, human adipose stem cells were isolated from subcutaneous fat in the thigh (htASCs), abdomen (haASCs) and breast (hbASCs). Flow cytometry was used to detect cell surface markers, and an enzyme-linked immunosorbent assay was used to detect paracrine activity. Paracrine gene expression in the three cell types was examined using real-time qPCR, and adipogenic ability was assessed using Oil Red O staining. RNA from third-passage haASCs and hbASCs was sequenced. The results showed that the differentiation potential marker markers CD49d and CD54 were similar across hbASCs from 10 subjects. The hbASCs showed higher colony forming ability and expression of fibroblast growth factor-2, tissue inhibitor of metalloproteinase-1 and stromal cell derived factor-1 than htASCs and haASCs. Stimulating hbASCs with FGF2 promoted adipogenic differentiation, while treating the cells with the PI3K inhibitor LY294002 inhibited differentiation. These results suggest that the PI3K/Akt signaling pathway can promote proliferation and adipogenic differentiation of adipose stem cells, and that activation of this pathway by FGF2 may explain why hbASCs show greater proliferation and adipogenic differentiation than haASCs and htASCs.

show abstract

“…To overcome this issue, an approach known as “cell sheet engineering” can be used; it relies on generation of scaffold-free tissue-like constructs consisting of cells and extracellular matrix as well as growth factors produced by the cells. Recently, this approach to cell delivery preserving viability and supporting cells’ regenerative functions, has been applied in experimental and clinical studies in regenerative medicine [24,25,26,27,28].…”

Section: Discussionmentioning

confidence: 99%

Transplantation of Adipose Stromal Cell Sheet Producing Hepatocyte Growth Factor Induces Pleiotropic Effect in Ischemic Skeletal Muscle

Boldyreva

Shevchenko

Molokotina

et al. 2019

IJMS

Self Cite

View full text Add to dashboard Cite

Cell therapy remains a promising approach for the treatment of cardiovascular diseases. In this regard, the contemporary trend is the development of methods to overcome low cell viability and enhance their regenerative potential. In the present study, we evaluated the therapeutic potential of gene-modified adipose-derived stromal cells (ADSC) that overexpress hepatocyte growth factor (HGF) in a mice hind limb ischemia model. Angiogenic and neuroprotective effects were assessed following ADSC transplantation in suspension or in the form of cell sheet. We found superior blood flow restoration, tissue vascularization and innervation, and fibrosis reduction after transplantation of HGF-producing ADSC sheet compared to other groups. We suggest that the observed effects are determined by pleiotropic effects of HGF, along with the multifactorial paracrine action of ADSC which remain viable and functionally active within the engineered cell construct. Thus, we demonstrated the high therapeutic potential of the utilized approach for skeletal muscle recovery after ischemic damage associated with complex tissue degenerative effects.

show abstract

Adipose-derived stem cell sheets functionalized by hybrid baculovirus for prolonged GDNF expression and improved nerve regeneration

Cited by 45 publications

References 57 publications

Brain Derived Neurotrophic Factor and Glial Cell Line-Derived Neurotrophic Factor-Transfected Bone Mesenchymal Stem Cells for the Repair of Periphery Nerve Injury

Brain Derived Neurotrophic Factor and Glial Cell Line-Derived Neurotrophic Factor-Transfected Bone Mesenchymal Stem Cells for the Repair of Periphery Nerve Injury

FGF2-induced PI3K/Akt signaling evokes greater proliferation and adipogenic differentiation of human adipose stem cells from breast than from abdomen or thigh

Transplantation of Adipose Stromal Cell Sheet Producing Hepatocyte Growth Factor Induces Pleiotropic Effect in Ischemic Skeletal Muscle

Contact Info

Product

Resources

About