Adipose-Derived Stem Cell–Enriched Lipotransfer Reverses Skin Sclerosis by Suppressing Dermal Inflammation

Abstract

cleroderma is a chronic autoimmune disease that results in skin and organ fibrosis and microvascular abnormalities, with an incidence of 2.7 per 100,000 people. 1,2 More than 90 percent of patients develop sclerotic skin changes such as dermatogenic contractures, sclerodactyly, perioral plication, microstomia, and mask-like facial stiffness. Lesions can develop further, leading to hair loss, diminished sweating, hyperpigmentation, depigmentation, or severe pruritus, all

Transplantation of in vitro prefabricated adipose organoids attenuates skin fibrosis by restoring subcutaneous fat and inducing dermal adipogenesis

“…In accordance with previous reports, 6 bleomycin (BLM) was administered to C57/BL6 mice in order to induce skin fibrosis. Briefly, 300 g/mL of BLM was dissolved in 0.9% phosphate‐buffered saline (PBS).…”

“…28 Thus, regeneration of subcutaneous adipose tissue is important for treatment of scleroderma. While a number of basic and clinical studies show the potential of fat transplantation to improve skin fibrosis in scleroderma, 6,29 the efficacy of this approach is not fully understood. Moreover, some juvenile LoS patients do not have sufficient adipose tissue for fat grafting.…”

“…The ad‐organoid group: adipose organoids (40 organoids embedded in 200 μL Matrigel) were injected into the same place of mice under the same condition. Four weeks later, mice were sacrificed and skin lesions were harvested 6,21 . In both the experimental procedures and data analysis, the researchers were blinded to the treatment group.…”

Brown adipose tissue transplantation improves skin fibrosis in localized scleroderma

“…In addition to being able to effectively reconstruct facial volume and limit the deposition of extracellular matrix proteins, fat grafts also have specific regenerative properties. Stem cell‐enriched implantation protocol amplifies the clinical effect of fat grafting for facial fibrosis 22 . However, the possible therapeutic effect of brown adipocytes on sclerosing skin lesions has been overlooked.…”

“…Stem cell-enriched implantation protocol amplifies the clinical effect of fat grafting for facial fibrosis. 22 However, the possible therapeutic effect of brown adipocytes on sclerosing skin lesions has been overlooked. BAT has less oil content, which is beneficial for reducing the inflammatory response after transplantation.…”