Adipose-derived stem cell (ASC)-enriched fat grafting: experiments using White rabbits and an automated cell processing apparatus

Abstract

The grafting of fat mixed with adipose-derived stem cells (ASCs) is being increasingly applied to compensate for the disadvantages of previous fat grafting methods. Devices that automatically isolate fat stem cells also have recently been developed. ASCs were isolated from the inguinal region of White rabbits using Icellator, and the number of cells and their viability were measured. The cell count per fat graft (mL) was adjusted to the following concentrations and subcutaneously transplanted into the back: Control group, Fat + PBS; Fat + ASCs (×0.5) group, 1.6 × 10 cells/mL; and Fat + ASCs (×1) group, 3.2 × 10 cells/mL. Grafted fat weight was measured after 8 weeks, and histological, immunohistological, and specifically stained sections were prepared. Fat absorption was reduced in Fat + ASCs (×0.5) and Fat + ASCs (×1) groups. The number of blood vessels was higher in Fat + ASCs (×1) than in the control group, and blood vessel areas were higher in Fat + ASCs (×0.5) and Fat + ASCs (×1) groups than in the control group. The usefulness of the automated cell processing apparatus, Icellator, was confirmed, and the results obtained suggest that grafted ASCs promote the vascularization and engraftment of fat grafts.

Adipose tissue-derived ECM hydrogels and their use as 3D culture scaffold

“…In this way, the clinical efficacy of lipografting is only temporary. To improve tissue grafting, scientists implemented a technique where they combined adipose tissuederived stromal cells (ASC) with fat tissue [5].…”

Evaluation of adipogenesis over time using a novel bioabsorbable implant without the addition of exogenous cells or growth factors

“…Adipose tissue is a major source of ASCs and mature adipocytes [ [13] , [14] , [15] , [16] , [17] ]. In clinical application, the addition of ASCs to fat grafts improves the survival rate [ 18 , 19 ]. However, there is no clear consensus on the effects of ASCs on tumor growth or the safety of these therapies in patients with cancer [ 20 ].…”

Mechanical Stress Improves Fat Graft Survival by Promoting Adipose-Derived Stem Cells Proliferation

“…Autologous fat grafting is a widely used procedure for soft tissue volume augmentation owing to its biocompatibility, natural texture, and versatility [ 1 , 2 , 3 ]. Despite these advantages, the engraftment rate of fat grafts ranges from 20–80% [ 3 , 4 ], which is the main limitation of fat grafting in reconstructive surgery.…”