Adipose-Derived Neural Stem Cells Combined with Acellular Dermal Matrix as a Neural Conduit Enhances Peripheral Nerve Repair

Syu, Wei-Ze; Hueng, Dueng‐Yuan; Chen, Wei‐Liang; Chan, James; Chen, Shyi-Gen; Huang, Shih‐Ming

doi:10.1177/0963689719853512

Cited by 13 publications

(15 citation statements)

References 37 publications

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“…Both Groups C and D improved nerve fiber density. It stays in line with other reports concerning the use of ASCs as an additive in nerve regeneration therapies [ 28 , 29 ]. The similarly beneficial effect of ASCs sheet enveloping a decellularized autograft was presented by Nakada et al; however, their solution did not exceed the axonal density of the comparator (1.8 vs. 2.29 axons/µm 2 ) as we observed in Group D vs. B in both stainings.…”

Section: Discussionsupporting

confidence: 91%

“…However, the addition of ASCs to the conduit slightly decreased the beneficial effect vs. the autograft group. It opposes other studies, where ASCs addition either to allografts or conduits was even more profitable for wet muscle mass ratio [ 27 , 28 , 29 ]. Yet, it was statistically significant that the differences between mean values of Groups B, C, and D remain much lower vs. similar studies.…”

Section: Discussionmentioning

confidence: 62%

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Bioactive Nanofiber-Based Conduits in a Peripheral Nerve Gap Management—An Animal Model Study

Dębski

Kijeńska‐Gawrońska

Zołocińska

et al. 2021

IJMS

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The aim was to examine the efficiency of a scaffold made of poly (L-lactic acid)-co-poly(ϵ-caprolactone), collagen (COL), polyaniline (PANI), and enriched with adipose-derived stem cells (ASCs) as a nerve conduit in a rat model. P(LLA-CL)-COL-PANI scaffold was optimized and electrospun into a tubular-shaped structure. Adipose tissue from 10 Lewis rats was harvested for ASCs culture. A total of 28 inbred male Lewis rats underwent sciatic nerve transection and excision of a 10 mm nerve trunk fragment. In Group A, the nerve gap remained untouched; in Group B, an excised trunk was used as an autograft; in Group C, nerve stumps were secured with P(LLA-CL)-COL-PANI conduit; in Group D, P(LLA-CL)-COL-PANI conduit was enriched with ASCs. After 6 months of observation, rats were sacrificed. Gastrocnemius muscles and sciatic nerves were harvested for weight, histology analysis, and nerve fiber count analyses. Group A showed advanced atrophy of the muscle, and each intervention (B, C, D) prevented muscle mass decrease (p < 0.0001); however, ASCs addition decreased efficiency vs. autograft (p < 0.05). Nerve fiber count revealed a superior effect in the nerve fiber density observed in the groups with the use of conduit (D vs. B p < 0.0001, C vs. B p < 0.001). P(LLA-CL)-COL-PANI conduits with ASCs showed promising results in managing nerve gap by decreasing muscle atrophy.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 62%

Bioactive Nanofiber-Based Conduits in a Peripheral Nerve Gap Management—An Animal Model Study

Dębski

Kijeńska‐Gawrońska

Zołocińska

et al. 2021

IJMS

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“…In the microscopic analysis, group D showed less pronounced brosis of the perineurium than the pure conduit. These observations stay in line with other reports concerning the use of ASC as an additive in nerve regeneration therapies [20,21]. The similarly bene cial effect of ASC sheet enveloping a decellularized autograft was presented by Nakada et al,…”

Section: Discussionsupporting

confidence: 91%

“…However, the addition of ASC to the conduit slightly decreased the bene cial effect vs autograft group. It opposes other studies, where ASC addition either to allografts or conduits was even more pro table for wet muscle mass ratio [19][20][21]. Yet, statistically signi cant, the differences between mean values of groups B, C, D remains much lower vs similar studies.…”

Section: Discussioncontrasting

confidence: 68%

Bioactive Nanofiber-based Conduits in a Peripheral Nerve Gap Management- an Animal Model Study.

Dębski

Kijeńska‐Gawrońska

Zołocińska

et al. 2020

Preprint

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Background: Peripheral nerve injuries (PNI) are trauma with severe squeals for patients’ quality of life. Currently, the gold standard of nerve reconstruction relies on primary coaptation. However, in case of a nerve gap, a reconstructive material for bridging is needed. Nerve conduits are an increasingly popular solution alternative to autografts or processed nerved allografts. The aim of the study was to examine the efficiency of a novel bioactive nanofiber-based tubular scaffold made of poly (L-lactic acid)-co-poly(ϵ-caprolactone), collagen, polyaniline and enriched with adipose-derived stem cells (ASCs) as a nerve conduit in a rat model.Methods: Poly (L-lactic acid)-co-poly(ϵ-caprolactone) scaffold was optimized and enriched with collagen (COL) and polyaniline (PANI) to create final (P(LLA-CL)-COL-PANI), tubular-shaped scaffold, manufactured with electrospinning technology. Parallelly, adipose tissue from 10 Lewis rats was harvested for ASC culture. 28 inbred male Lewis rats were divided into four even groups. Each animal underwent sciatic nerve transection and excision of a 10 mm nerve trunk fragment. In group A, nerve gap remained untouched, in B excised trunk was rotated and used as an autograft, in C nerve stumps were secured with P(LLA-CL)-COL-PANI conduit. In the D group gap was reconstructed with P(LLA-CL)-COL-PANI conduit enriched with ASCs. After 6-months of observation rats were sacrificed. Gastrocnemius muscles (operated and non-operated side) and reconstructed sciatic nerves were harvested for analyses. Muscles were weighted and underwent histological analysis. Nerves were processed and stained immunohistochemically with NF-200 to be analyzed with dedicated software for nerve fiber count. Results: No signs of rejection or excessive fibrosis was noted. Muscle mass ratio was highest in group B (0.77±0.05), then in C (0.74±0,04) and D (0.67± 0.07). Group A showed advanced atrophy of the muscle, each intervention prevented muscle mass decrease (p<0.0001), however, ASC addition decreased efficiency vs autograft (p<0.05). Nerve fiber count revealed a superior effect in the nerve fiber density observed in the group with the use of conduit vs autograft (D vs B p<0.0001, C vs B p<0.001). ASC added to the conduit decreased perineurium hyperplasia.Conclusion: P(LLA-CL)-COL-PANI conduits with ASC showed promising results in managing nerve gap by decreasing muscle atrophy and providing a favorable environment for peripheral nerve regeneration.

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“…Such co-culture was demonstrated as a better therapeutic strategy to repair peripheral nerve injuries when compared to using neurospheres alone. 23 Furthermore, neurospheres have been tested in several rodent transplantation studies targeting tissue regeneration in disease including Alzheimer’s disease, macular degeneration, and spinal cord injury ( Table 1 ). Interestingly, transplanted neurospheres were better at restoring native cell populations compared to the transplantation of undifferentiated cells and helped improve certain behavioral phenotypes in cognitively impaired animals.…”

Section: Neurospheres: Simplified Human Cns Spheroidsmentioning

confidence: 99%

Human mini brains and spinal cords in a dish: Modeling strategies, current challenges, and prospective advances

et al. 2022

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Engineered three-dimensional (3D) in vitro and ex vivo neural tissues, also known as “mini brains and spinal cords in a dish,” can be derived from different types of human stem cells via several differentiation protocols. In general, human mini brains are micro-scale physiological systems consisting of mixed populations of neural progenitor cells, glial cells, and neurons that may represent key features of human brain anatomy and function. To date, these specialized 3D tissue structures can be characterized into spheroids, organoids, assembloids, organ-on-a-chip and their various combinations based on generation procedures and cellular components. These 3D CNS models incorporate complex cell-cell interactions and play an essential role in bridging the gap between two-dimensional human neuroglial cultures and animal models. Indeed, they provide an innovative platform for disease modeling and therapeutic cell replacement, especially shedding light on the potential to realize personalized medicine for neurological disorders when combined with the revolutionary human induced pluripotent stem cell technology. In this review, we highlight human 3D CNS models developed from a variety of experimental strategies, emphasize their advances and remaining challenges, evaluate their state-of-the-art applications in recapitulating crucial phenotypic aspects of many CNS diseases, and discuss the role of contemporary technologies in the prospective improvement of their composition, consistency, complexity, and maturation.

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Adipose-Derived Neural Stem Cells Combined with Acellular Dermal Matrix as a Neural Conduit Enhances Peripheral Nerve Repair

Cited by 13 publications

References 37 publications

Bioactive Nanofiber-Based Conduits in a Peripheral Nerve Gap Management—An Animal Model Study

Bioactive Nanofiber-Based Conduits in a Peripheral Nerve Gap Management—An Animal Model Study

Bioactive Nanofiber-based Conduits in a Peripheral Nerve Gap Management- an Animal Model Study.

Human mini brains and spinal cords in a dish: Modeling strategies, current challenges, and prospective advances

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