Adipose Derived Mesenchymal Stem Cells Efficiently Rescue Carbon Tetrachloride-Induced Acute Liver Failure in Mouse

Abstract

Background and Aim. Adipose derived mesenchymal stem cells (ADMSCs) may be an attractive source for acute and chronic liver injury because they are abundant and easy to obtain. We aim to investigate the efficacy of ADMSCs transplantation in the acute liver failure (ALF) caused by carbon tetrachloride (CCl4) in mice. Methods. ADMSCs were isolated from inguinal fat pads of enhanced green fluorescent protein (EGFP) transgenic mice and their surface markers and differentiation potential were analyzed. ALF models were established by infusion of CCl4 and divided into two groups: control group; EGFP-ADMSCs transplantation group. The restoration of biological functions of the livers receiving transplantation was assessed via a variety of approaches such as survival rates, live function parameters, histological localization of EGFP-ADMSCs, and Immunofluorescence analysis. Results. ADMSCs were positive for CD105, CD44 but negative for CD45, CD34 and had adipogenic, osteogenic differentiation potential. The survival rate of transplantation group significantly increased compared to PBS group. Furthermore, the transplanted cells were well integrated into injured livers and produced albumin, cytokeratin-18. Conclusion. Direct transplantation of ADMSCs is an effective treatment for ALF. The transplanted ADMSCs exhibit the potential to differentiate into hepatocyte-like cells in the injured livers.

Poly (dopamine) coated superparamagnetic iron oxide nanocluster for noninvasive labeling, tracking and targeted delivery of adipose tissue-derived stem cells

“…Considerably, compared with those without ADSC injection, less incidences of inflammatory infiltration were appeared around the liver central vein of treated mice after tail intravenous injection of the SPIONs cluster@PDA-labeled ADSCs for 24 hrs, implying that the labeled ADSCs could promote the repair of injured liver. These results are in well consistent with the previous studies that unlabeled ADSCs can ameliorate the liver injuries induced by CCl 4 in rodent model 39 40 41 42 . Hence, above data indicate the therapeutic effects of ADSCs on liver injures cannot be affected by the labeling of SPIONs cluster@PDA nanoparticles, and also proved the good biocompatibility of these nanoparticles.…”

Pretreatment of Adipose Derived Stem Cells with Curcumin Facilitates Myocardial Recovery via Antiapoptosis and Angiogenesis

“…It is well-known that BMSCs play the cardioprotective roles mainly through paracrine, differentiation into specialized cardiac cell lineages, and recruitment of endogenous stem cells [ 6 , 7 ]. Since adipose derived mesenchymal stem cells (ADSCs) have proven to be abundant, easy to isolate and obtain repeatedly, minimally invasive properties and particularly grow old later than BMSCs, ADSCs have been an appealing cell source in regenerative medicine [ 8 – 10 ]. However, very low survival rate of transplanted cells is an impediment of successful heart cell therapy [ 11 ].…”

Antifibrotic Activity of Human Placental Amnion Membrane-Derived CD34+ Mesenchymal Stem/Progenitor Cell Transplantation in Mice With Thioacetamide-Induced Liver Injury

“…The antifibrotic effect of the CD34 + AMSPC transplantation was proven to alleviate the injury in the mice with severely damaged livers. It is worth noting that ADMSCs have been reported to exert beneficial effects in other animal models of acute liver failure when infused through the tail vein [54]. Administration through the spleen may not be the ideal route for ADMSCs.…”