Adipose-derived mesenchymal stem cells from infrapatellar fat pad of patients with rheumatoid arthritis and osteoarthritis have comparable immunomodulatory properties

Abstract

Adipose-derived mesenchymal stem cells (ASCs) possess immunosuppressive properties, but their activity is dependent on stimuli provided by local environment. It is possible that proinflammatory milieu of rheumatoid joint affects ASCs function. To verify this hypothesis, rheumatoid ASCs (RA-ASCs) and osteoarthritic ASCs (OA-ASCs) derived from infrapatellar fat pad (IPFP) of the knee joint have been compared. RA- and OA-ASCs isolated from patients were cultured in vitro. Their secretory and proliferative activity was measured. Peripheral blood mononuclear cells (PBMCs) from healthy donors were co-cultured with ASCs. Then, PBMCs proliferation was measured by (3)H-thymidine incorporation method, cytokines secretion by immunoassays, T cells activation and regulatory T cells (Tregs) percentage - by flow cytometry. RA- and OA-ASCs properties in vitro were comparable, however, some differences in secretory activity occurred. RA- and OA-ASCs inhibited PBMCs proliferation and induced interleukin 10 production but up-regulated interleukin 17 A secretion and failed to limit release of other proinflammatory mediators (tumor necrosis factor [TNF], interferon γ [IFNγ], CCL5) by PBMCs. RA- and OA-ASCs did not suppress activation markers expression on T cells and did not trigger Tregs expansion. The present study shows that IPFP-ASCs from RA and OA patients have comparable functions in vitro. Their immunosuppressive activity seems to be impaired comparing to available data.

Exosomes: roles and therapeutic potential in osteoarthritis

“…Exosomes from adipose tissue mesenchymal stem cells: Adipose tissue mesenchymal stem cells (AMSCs) were demonstrated to have potent capability for cartilage regeneration and inflammatory modulation and are considered an excellent cell source for OA treatment. [202][203][204][205][206][207] However, the mechanisms of AMSC-induced cartilage regeneration are still not clear. Increasing evidence suggests that AMSCs may prevent cartilage erosion and improve joint function primarily through the paracrine secretion of trophic factors to regulate the local microenvironment, making it more favorable for repair and regeneration.…”

Association between the polymorphism of IL-17A and IL-17F gene with knee osteoarthritis risk: a meta-analysis based on case-control studies

“…Interleukin-17 (IL-17) families include six members (A, B, C, D, E, F), and they play active roles in autoimmune diseases and inflammatory diseases [8,9]. IL-17A is a member of IL-17 families and is a pro-inflammatory cytokine associated with many inflammatory diseases, such as rheumatoid arthritis (RA), ankylosing spondylitis, and systemic lupus erythematosus [10].…”

Clinical Efficacy of Arthroscopic Therapy With Infrapatellar Fat Pad Cell Concentrates in Treating Knee Cartilage Lesion: a Randomized Controlled Trial

“…According to the study by Liu et al [38], it is possible to generate robust, exible cartilage-like grafts of scale, opening up the possibility that tissues engineered using IPFP-MSCs derived from OA patients could potentially be used to resurface large areas of joint surfaces damaged by trauma or disease. Skalska U et al [39] shows that MSCs derived from IPFP of rheumatoid arthritis (RA) patients have comparable or slightly stronger osteogenic potential than that from osteoarthritis patients. Koh et al [40,41] launched several stem cell injections combined with arthroscopic debridement to patients with knee OA.…”