Adipose-derived Mesenchymal Stem Cells and Their Reparative Potential in Ischemic Heart Disease

Badimón, Lina; Oñate, Blanca; Vilahur, Gemma

doi:10.1016/j.rec.2015.02.025

Cited by 37 publications

(41 citation statements)

References 131 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Over the last years, fresh, uncultured, unmodified, autologous adipose-derived regenerative cells (UA-ADRCs) have become highly attractive for the practice of regenerative cell therapy (e.g., [6][7][8][9][10]) (note that fresh, uncultured, unmodified adipose-derived regenerative cells were also named "stromal vascular fraction" (SVF) in many publications; e.g., [11][12][13]). This is due to the fact that UA-ADRCs have several advantages over other types of cells used in and/or under investigation for regenerative cell therapy.…”

Section: Introductionmentioning

confidence: 99%

Isolation of adipose tissue derived regenerative cells from human subcutaneous tissue with or without the use of an enzymatic reagent

Valenzuela

Alt

Winnier

et al. 2018

Preprint

View full text Add to dashboard Cite

Background: Freshly isolated, unmodified autologous adipose derived regenerative cells (ADRCs) have emerged as a promising tool for regenerative cell therapy. The Transpose RT system (InGeneron, Inc., Houston, TX, USA) is a system for isolating ADRCs from adipose tissue, commercially available in Europe as a CE-marked medical device and under clinical evaluation in the United States. This system makes use of the proprietary, enzymatic Matrase Reagent for isolating cells. The present study addressed the question whether the use of Matrase Reagent influences cell yield, cell recovery, cell viability, biological characteristics, physiological functions or structural properties of the ADRCs in final cell suspension. Methods: Identical samples of subcutaneous adipose tissue from 12 subjects undergoing elective lipoplasty were processed either with or without the use of Matrase Reagent. Then, characteristics of the ADRCs in the respective final cell suspensions were evaluated. Results: Compared to non-enzymatic isolation, enzymatic isolation resulted in approximately twelve times higher mean cell yield (i.e., numbers of viable cells/ml lipoaspirate) and approximately 16 times more colony forming units (CFUs). Despite these differences, cells isolated from lipoaspirate both with and without the use of Matrase Reagent were independently able to differentiate into cells of all three germ layers. Discussion: The data of this study indicate that biological characteristics, physiological functions or structural properties relevant for the intended use were not altered or induced using Matrase Reagent. A comprehensive literature review demonstrated that isolation of ADRCs from lipoaspirate using the Transpose RT system and the Matrase Reagent results in the highest viable cell yield among all published data regarding isolation of ADRCs from lipoaspirate. ABSTRACTBackground: Freshly isolated, unmodified autologous adipose derived regenerative cells (ADRCs) have emerged as a promising tool for regenerative cell therapy. The Transpose RT system (InGeneron, Inc., Houston, TX, USA) is a system for isolating ADRCs from adipose tissue, commercially available in Europe as a CE-marked medical device and under clinical evaluation in the United States. This system makes use of the proprietary, enzymatic Matrase Reagent for isolating cells. The present study addressed the question whether the use of Matrase Reagent influences cell yield, cell recovery, cell viability, biological characteristics, physiological functions or structural properties of the ADRCs in final cell suspension. Methods: Identical samples of subcutaneous adipose tissue from 12 subjects undergoing elective lipoplasty were processed either with or without the use of Matrase Reagent. Then, characteristics of the ADRCs in the respective final cell suspensions were evaluated. Results: Compared to non-enzymatic isolation, enzymatic isolation resulted in approximately twelve times higher mean cell yield (i.e., numbers of viable cells/ml lipoaspirate) and approximately 16 tim...

show abstract

Section: Introductionmentioning

confidence: 99%

Isolation of adipose tissue derived regenerative cells from human subcutaneous tissue with or without the use of an enzymatic reagent

Valenzuela

Alt

Winnier

et al. 2018

Preprint

View full text Add to dashboard Cite

show abstract

“…Scientific evidence shows that they are one of the best choices in cell therapy, because of their ease of access and isolation, great potential of expansion in culture, immunosuppressive properties, paracrine effect and ability to migrate to injured tissues [52]. Moreover, their great therapeutic potential has been documented in the repair and regeneration of injured tissues in nearly every organ of the body, including the heart [53], immune system [54], liver [55], kidneys [56] and bone and cartilage tissue [57].…”

Section: Mesenchymal Stem Cells-based Therapymentioning

confidence: 99%

“…MSCs are a group cells that have been isolated from virtually every vascularized tissue [62]. MSCs are a group cells that have been isolated from virtually every vascularized tissue [52]; however, recent reports have documented that they can also be isolated from other sources as umbilical cord [62], peripheral blood [63], adipose tissue [64][65][66][67], hair follicle [68], periodontal ligament [69][70][71][72], gingival tissue [73] and dental pulp [74,75], among others.…”

Section: Mesenchymal Stem Cells-based Therapymentioning

confidence: 99%

Regenerative Medicine: A New Paradigm in Bone Regeneration

Chaparro¹,

Linero²

2016

Advanced Techniques in Bone Regeneration

View full text Add to dashboard Cite

Bone defects are the cause of functional disability and the restoration of skeletal function remains an important challenge on orthopedics, neurosurgery and oral and maxillofacial surgery. Because of the limitations of the currently used techniques for the reconstruction of bone defects and the difficulties for the implementation of new therapeutic strategies, a new paradigm in the field of reconstructive surgery has arisen, leading to tissue engineering and regenerative medicine. Mesenchymal stem cells (MSC) have emerged as a promising alternative for the treatment of bone lesions. It was postulated that the therapeutic action was the result of proliferation and differentiation of MSCs, replacing injured tissue. However, recent studies have shown that MSCs secrete a number of trophic factors that have a strong effect during repair and tissue regeneration. This represents a shift from a paradigm centered on MSC proliferation and differentiation to a new paradigm in which the MSCs exert their beneficial effect by the secretion of paracrine factors that induce endogenous repair mechanisms. This chapter will bring together basic and clinical aspects, focused on novel findings on MSC paracrine effect and the development of new therapeutic strategies based on growth factors, cytokines and signaling molecules involved in bone regeneration.

show abstract

“…Cells derived from adipose tissue, either freshly isolated (named stromal vascular fraction (SVF) or adipose-derived regenerative cells (ADRCs)) or culture-expanded (named adipose-derived stem cells (ASCs)) have emerged as a promising tool for regenerating ischemic myocardium after MI 5,17 . Both ADRCs and ASCs have a number of advantages over other cell types in cell-based therapies for MI.…”

Section: Introductionmentioning

confidence: 99%

“…Both ADRCs and ASCs were applied in a number of experimental studies using rodent, rabbit, porcine and sheep animal models 17 ; details of studies using porcine and sheep animal models are summarized in Supplementary Table 1. Porcine and sheep models of experimentally induced transmural MI have the great advantage over rodent and rabbit models that potential therapeutic application of ADRCs and ASCs can be performed using the same instrumentation and standard of care as in humans 25 .…”

Section: Introductionmentioning

confidence: 99%

Unmodified, autologous adipose-derived regenerative cells improve cardiac function, structure and revascularization in a porcine model of chronic myocardial infarction

Haenel

Ghosn

Karimi

et al. 2018

Preprint

View full text Add to dashboard Cite

Numerous studies have investigated cell-based therapies for myocardial infarction (MI), with mixed results. In the present study the left anterior descending (LAD) artery of pigs was occluded for 180 min. Four weeks later, the mean left ventricular ejection fraction (LVEF) was shown to have been reduced to approximately 35%. At that time, 18×106 unmodified, autologous adipose-derived regenerative cells (UA-ADRCs) were delivered into the LAD vein (control: delivery of saline). Six weeks following UA-ADRCs/saline delivery, the mean LVEF had increased by 18% (p<0.01) after delivery of UAADRCs, but was unchanged after delivery of saline. This is among the best outcome ever reported in studies on porcine animal models of cell-based therapies for MI in which functional outcome was assessed with cardiac magnetic resonance imaging. The unique combination of the procedure used for isolating UA-ADRCs, the late cell delivery time and the uncommon cell delivery route applied in the present study may open new horizons for cell-based therapies for MI.

show abstract

Adipose-derived Mesenchymal Stem Cells and Their Reparative Potential in Ischemic Heart Disease

Cited by 37 publications

References 131 publications

Isolation of adipose tissue derived regenerative cells from human subcutaneous tissue with or without the use of an enzymatic reagent

Isolation of adipose tissue derived regenerative cells from human subcutaneous tissue with or without the use of an enzymatic reagent

Regenerative Medicine: A New Paradigm in Bone Regeneration

Unmodified, autologous adipose-derived regenerative cells improve cardiac function, structure and revascularization in a porcine model of chronic myocardial infarction

Contact Info

Product

Resources

About