Adipose-derived mesenchymal stem cell seeded Atelocollagen scaffolds for cardiac tissue engineering

Li, Qiong; Li, Miaomiao; Li, Meng; Zhang, Zhengyan; Ma, Han; Zhao, Liang; Zhang, Min; Wang, Guodong

doi:10.1007/s10856-020-06425-2

Cited by 5 publications

(4 citation statements)

References 34 publications

(36 reference statements)

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“…substrate for mesenchymal stem cell attachment and enhancing chondrogenic differentiation [23,24]. However, the precise mechanism of healing effect after atelocollagen injection is yet to be clarified.…”

Section: Discussionmentioning

confidence: 99%

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A Clinical Pilot Study Showing the Safety and Efficacy of Intramuscular Injection of Atelocollagen for Prevention of Paraspinal Muscle Atrophy after Spine Surgery

Park

Hur

Hong

et al. 2022

J Minim Invasive Spine Surg Tech

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The objective of this study was to assess the safety and efficacy of intramuscular injection of atelocollagen for the prevention of paraspinal muscle atrophy after spine surgery. Atelcollagen has been widely used as an intradermal filler to restore soft tissue defect. Many studies demonstrated that atelocollagen provides good therapeutic results by promoting cell proliferation and enhances the healing effect on injured connective tissues such as tendons and fasciae, while causing few complications. Methods: A total of 118 patients who underwent single level of posterior lumbar interbody fusion (PILF) between December 2017 and April 2019 were retrospectively reviewed. In the study group of 60 patients, 3 mL of gel-type 3% atelocollagen solution was prepared and injected into the multifidus muscle during wound closure. Clinical efficacy was evaluated by the improvement of back pain, elevation of a muscle enzyme, and inflammatory markers. Radiologic efficacy was evaluated with a comparison of density and cross-sectional area (CSA) of multifidus and erector spinae muscle in CT images. Results: Visual analogue scale (VAS) scores for back pain was not significantly lower in the study group postoperatively compared with the control group. The reduction of postoperative paraspinal muscle density and CSA was significantly lower in the study group. The serum level of muscle enzyme and inflammatory markers were significantly lower in the study group. No major procedure-related complications were observed during the follow-up period. Conclusion: Intramuscular injection of atelocollagen is safe and feasible for the prevention of paraspinal muscle atrophy after spine surgery. This novel method seems advantageous for accelerating wound healing without causing inflammation.

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Section: Discussionmentioning

confidence: 99%

“…Several studies have demonstrated surgical role of atelocollagen and promising clinical results have been reported [8,16,21,23,25]. In a recent study using atelocollagen in orthopaedic surgery, Suh et al [26] suggested repair after using patch-type atelocollagen between the torn rotator cuff and bone using rabbits.…”

Section: Discussionmentioning

confidence: 99%

A Clinical Pilot Study Showing the Safety and Efficacy of Intramuscular Injection of Atelocollagen for Prevention of Paraspinal Muscle Atrophy after Spine Surgery

Park

Hur

Hong

et al. 2022

J Minim Invasive Spine Surg Tech

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show abstract

“…Several previous studies reported the increased differentiation potential of AD-MSCs into functional contractile smooth muscle cells ( Rodriguez et al, 2006 ; Heydarkhan-Hagvall et al, 2008 ; Harris et al, 2011 ), which were encapsulated with biomaterial-based hydrogels and proliferated on decellularized blood vessel scaffolds, suggesting their capacity for regenerating the vascular structures and functions ( Harris et al, 2011 ). Li et al (2020) implanted AD-MSCs on atelocollagen scaffolds, induced differentiation into cardiocyte-like cells using 5-azacytidine, and subsequently transplanted into mice with ischemic myocardium. Krawiec et al (2017) also fabricated biodegradable, elastomeric, porous vascular scaffolds using non-toxic, cytocompatible poly (ester urethane) urea.…”

Section: Vascularized Tissue Regeneration and Repairmentioning

confidence: 99%

Enhancing Stem Cell-Based Therapeutic Potential by Combining Various Bioengineering Technologies

Hong

2022

Front. Cell Dev. Biol.

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Stem cell-based therapeutics have gained tremendous attention in recent years due to their wide range of applications in various degenerative diseases, injuries, and other health-related conditions. Therapeutically effective bone marrow stem cells, cord blood- or adipose tissue-derived mesenchymal stem cells (MSCs), embryonic stem cells (ESCs), and more recently, induced pluripotent stem cells (iPSCs) have been widely reported in many preclinical and clinical studies with some promising results. However, these stem cell-only transplantation strategies are hindered by the harsh microenvironment, limited cell viability, and poor retention of transplanted cells at the sites of injury. In fact, a number of studies have reported that less than 5% of the transplanted cells are retained at the site of injury on the first day after transplantation, suggesting extremely low (<1%) viability of transplanted cells. In this context, 3D porous or fibrous national polymers (collagen, fibrin, hyaluronic acid, and chitosan)-based scaffold with appropriate mechanical features and biocompatibility can be used to overcome various limitations of stem cell-only transplantation by supporting their adhesion, survival, proliferation, and differentiation as well as providing elegant 3-dimensional (3D) tissue microenvironment. Therefore, stem cell-based tissue engineering using natural or synthetic biomimetics provides novel clinical and therapeutic opportunities for a number of degenerative diseases or tissue injury. Here, we summarized recent studies involving various types of stem cell-based tissue-engineering strategies for different degenerative diseases. We also reviewed recent studies for preclinical and clinical use of stem cell-based scaffolds and various optimization strategies.

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“…Hence, porcine atelocollagen gel beds have been shown to improve human mesenchymal stem cells (hMSCs) chondrogenic differentiation [ 27 ], and fibril coatings using Bester sturgeon atelocollagen and MC3T3-E1 cells are proven as interesting tools for in vitro studies [ 28 ]. Three dimensionally cultured adipose tissue-derived mesenchymal stem cells (ADMSCs), on honeycomb scaffolds of atelocollagen from cattle, have demonstrated good biocompatibility with host cardio tissue in in vivo [ 29 ], and atelocollagen suspended in mesenchymal stem cells (MSCs) secretome implants enhanced the migration of endogenous stem cells in the rat calvarial bone defect model [ 30 ]. Besides, injectable forms of atelocollagen are being assayed in the clinic and outcomes recently reported for meniscal root repair [ 31 ] and gingival soft tissue regeneration [ 32 ].…”

Section: Introductionmentioning

confidence: 99%

Nanofibrous Gelatin-Based Biomaterial with Improved Biomimicry Using D-Periodic Self-Assembled Atelocollagen

2021

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Design of bioinspired materials that mimic the extracellular matrix (ECM) at the nanoscale is a challenge in tissue engineering. While nanofibrillar gelatin materials mimic chemical composition and nano-architecture of natural ECM collagen components, it lacks the characteristic D-staggered array (D-periodicity) of 67 nm, which is an important cue in terms of cell recognition and adhesion properties. In this study, a nanofibrous gelatin matrix with improved biomimicry is achieved using a formulation including a minimal content of D-periodic self-assembled atelocollagen. We suggest a processing route approach consisting of the thermally induced phase separation of the gelatin based biopolymeric mixture precursor followed by chemical-free material cross-linking. The matrix nanostructure is characterized using field emission gun scanning electron microscopy (FEG-SEM), transmission electron microscopy (TEM), wide angle X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FT-IR). The cell culture assays indicate that incorporation of 2.6 wt.% content of D-periodic atelocollagen to the gelatin material, produces a significant increase of MC3T3-E1 mouse preosteoblast cells attachment and human mesenchymal stem cells (hMSCs) proliferation, in comparison with related bare gelatin matrices. The presented results demonstrate the achievement of an efficient route to produce a cost-effective, compositionally defined and low immunogenic “collagen-like” instructive biomaterial, based on gelatin.

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Adipose-derived mesenchymal stem cell seeded Atelocollagen scaffolds for cardiac tissue engineering

Cited by 5 publications

References 34 publications

A Clinical Pilot Study Showing the Safety and Efficacy of Intramuscular Injection of Atelocollagen for Prevention of Paraspinal Muscle Atrophy after Spine Surgery

A Clinical Pilot Study Showing the Safety and Efficacy of Intramuscular Injection of Atelocollagen for Prevention of Paraspinal Muscle Atrophy after Spine Surgery

Enhancing Stem Cell-Based Therapeutic Potential by Combining Various Bioengineering Technologies

Nanofibrous Gelatin-Based Biomaterial with Improved Biomimicry Using D-Periodic Self-Assembled Atelocollagen

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