Adipose circular RNAs exhibit dynamic regulation in obesity and functional role in adipogenesis

Walet, Arcinas Camille Esther; Tan, Wilson Lek Wen; Fang, Wen-Ning; Desai, Tresha P; Teh, Diana Chee Siang; Degirmenci, Ufuk; Xu, Dan; Foo, Roger; Sun, Li

doi:10.1038/s42255-019-0078-z

Cited by 84 publications

(91 citation statements)

References 54 publications

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“…So far, transcriptome sequencing as a preferred biotechnique permits us to identify the circRNAs in diverse species [26][27][28][29][30], some of the circRNAs proved to play a vital role in animal growth and development. Recently,circRNA expression profiles have been characterized in adipose tissues of swine [31], mice [32], humans [33,34], buffalo [35] and cattle [28]. Nevertheless, the circRNAs in yak remain unknown.…”

Section: Discussionmentioning

confidence: 99%

CircRNA Expression Profile during Yak Adipocyte Differentiation and Screen Potential circRNAs for Adipocyte Differentiation

Zhang

Guo

Pei

et al. 2020

Genes

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The yak (Bos grunniens) is subjected to nutritional deficiency during the whole winter grazing season; deciphering the adipose metabolism and energy homeostasis under cold and nutrients stress conditions could be a novel way to understand the specific mechanism of energy metabolism. Circular RNAs (circRNAs) have elucidated that they play a key role in many biological events, but the regulatory function of adipose development remains mostly unknown. Therefore, the expression pattern of circRNAs were identified for the first time during yak adipocyte differentiation to gain insight into their potential functional involvement in bovine adipogenesis. We detected 7203 circRNA candidates, most of them contained at least two exons, and multiple circRNA isoforms could be generated from one parental gene. Analysis of differential expression circRNAs displayed that 136 circRNAs were differentially expressed at day 12 (Ad) after adipocyte differentiation, compared with the control at day 0 (Pread 0), while 7 circRNAs were detected on day 2. Sanger sequencing validated that six circRNAs had head-to-tail junction, and quantitative real-time PCR (qPCR) results revealed that the expression patterns of ten circRNAs were consistent with their expression levels from RNA-sequencing (RNA-seq) data. We further predicted the networks of circRNA-miRNA-gene based on miRNAs sponging by circRNAs, in which genes were participated in the adipocyte differentiation-related signaling pathways. After that, we constructed several adipocyte differentiation-related ceRNAs and revealed six circRNAs (novel_circ_0009127, novel_circ_0000628, novel_circ_0011513, novel_circ_0010775, novel_circ_0006981 and novel_circ_0001494) were related to adipogenesis. Furthermore, we analyzed the homology among yak, human and mouse circRNAs and found that 3536 yak circRNAs were homologous to human and mouse circRNAs. In conclusion, these findings provide a solid basis for the investigation of yak adipocyte differentiation-related circRNAs and serve as a great reference to study the energy metabolism of high-altitude animals.

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Section: Discussionmentioning

confidence: 99%

CircRNA Expression Profile during Yak Adipocyte Differentiation and Screen Potential circRNAs for Adipocyte Differentiation

Zhang

Guo

Pei

et al. 2020

Genes

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“…Abdominal and intramuscular fat deposition resulted from a variety of complex molecular mechanisms. Notably, tissue-speci c adipogenesis is potentially regulated by circRNAs [25]. In the present study, we identi ed DE circRNAs across various tissue and cell types.…”

Section: Discussionmentioning

confidence: 86%

“…circ-ZNF609, when translated to a functional petide, regulates myogenesis in humans and mice [24]. circArhgap5-2 plays a crucial role in adipogenesis and obesity [25]. However, circRNAs related to poultry adipogenesis and tissues-speci c fat deposition remain poorly studied.…”

Section: Introductionmentioning

confidence: 99%

Integrative analysis of circRNAs, miRNAs, and mRNAs profiles to reveal ceRNAs networks in chicken intramuscular and abdominal adipogenesis

Zhang

Han

Zhai

et al. 2020

Preprint

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Background: Tissue-specific fat deposition is regulated by a series of complex regulatory mechanisms. Reports indicate that epigenetic regulators, such as circular RNAs (circRNAs), are crucial in diseases progression, animal development, metabolism, and adipogenesis. In this study, to assess the functional roles of circRNAs in adipogenesis and tissue-specific fat deposition, we comprehensively analyzed the Ribo-Zero RNA-Seq and miRNAs data during chicken intramuscular and abdominal adipogenic differentiation. Results: circRNAs and miRNAs profiles during chicken adipogenic differentiation were found in adipocytes derived from various adipose tissues. It was also discovered that high levels of downregulated miRNAs potentially promote adipogenesis by activating their target genes which are associated with fatty acid metabolism and adipogenic differentiation. Through analysis of the correlation between the expression levels of circRNAs and adipogenic genes, as well as the dynamic expression patterns of circRNAs during adipogenic differentiation, several candidate circRNAs were identified. Moreover, competing endogenous RNA (ceRNAs) networks were constructed during chicken intramuscular and abdominal adipogenesis by combining miRNAs with mRNAs data. Several candidate circRNAs potentially influence adipogenesis by regulating miRNAs via PPAR and fatty acid metabolism-related pathways were identified, such as circLCLAT1, circFNDC3AL, circCLEC19A and circARMH1. Conclusion: In conclusion, our findings reveal that circRNAs and the circRNA-miRNAs-mRNAs-ceRNAs network may play important roles in chicken adipocytes differentiation and tissue-specific fat deposition.

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“…CircINSR adsorb miR-34a to promote the proliferation of bovine myoblasts (17). CircTshz2-1 and circArhgap5-2 have been shown to be indispensable regulators of fat formation (18). Despite these ndings, the role of circRNAs in muscle and IMF development still requires further research.…”

Section: Introductionmentioning

confidence: 99%

CircINSR regulates fetal bovine muscle and fat development

Shen

Tang

et al. 2020

Preprint

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Background: The level of muscle development directly affects the production efficiency of livestock, and the content of intramuscular fat (IMF) is an important factor affecting meat quality. Nevertheless, the molecular mechanism of embryonic circular RNA in muscle and IMF development remains largely unknown. Methods: In this study, we isolated myoblasts and intramuscular preadipocytes from fetal bovine skeletal muscle. Oil Red O and BODIPY staining were used to identify lipid droplets of preadipocytes, and anti-MyHC immunofluorescence was used to identify myotubes differentiated from myoblasts. Bioinformatics, dual fluorescence reporter system, and RNA immunoprecipitation were used to determine the interactions between circINSR and miR-15/16 family. Molecular and biochemical assays were used to confirm the role of circINSR in myoblasts and intramuscular preadipocytes. Results: We found that the isolated myoblasts and preadipocytes can differentiate normally. Besides, circINSR severed as a sponge of miR-15/16 family, which targeting CyclinD1 and Bcl-2. CircINSR overexpression significantly promoted myoblasts and preadipocytes proliferation, and inhibited cell apoptosis. In addition, circINSR inhibited preadipocytes adipogenesis by alleviating the inhibition of miR-15/16 on target genes FOXO1 and EPT1. Conclusions: Taken together, our study demonstrated circINSR as a regulator of embryonic muscle and IMF development.

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Adipose circular RNAs exhibit dynamic regulation in obesity and functional role in adipogenesis

Cited by 84 publications

References 54 publications

CircRNA Expression Profile during Yak Adipocyte Differentiation and Screen Potential circRNAs for Adipocyte Differentiation

CircRNA Expression Profile during Yak Adipocyte Differentiation and Screen Potential circRNAs for Adipocyte Differentiation

Integrative analysis of circRNAs, miRNAs, and mRNAs profiles to reveal ceRNAs networks in chicken intramuscular and abdominal adipogenesis

CircINSR regulates fetal bovine muscle and fat development

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