Adipose Autologous Micrograft and Its Derived Mesenchymal Stem Cells in a Bio Cross-Linked Hyaluronic Acid Scaffold for Correction Deep Wrinkles, Facial Depressions, Scars, Face Dermis and Its Regenerations: A Pilot Study and Cases Report

Abstract

The aim of this clinical study was to demonstrate that through a micrograft of viable adipose tissue cells microfiltered at 50 microns to exclude fibrous shoots and cell debris in a suspension of cross-linked hyaluronic acid, we were able to improve visible imperfections of the dermis and to improve clinically observable wrinkles, with a beneficial effect also in the extracellular matrix (ECM). Background and Objectives: With the passage of time, the aging process begins, resulting in a progressive impairment of tissue homeostasis. The main reason for the formation of wrinkles is the involution of the papillary dermis, as well as the loss of stem cell niches with compromise of the extra-cytoplasmic matrix (ECM), and the loss of hyaluronic acid, which helps to maintain the shape and resistance and that is contained in the connective tissue. Materials and Methods: This study involved 14 female patients who underwent dermal wrinkle correction and bio-regeneration over the entire facial area through a suspension containing 1.0 mL of viable micrografts from adipose tissue in a 1.0 mL cross-linked hyaluronic acid. To verify the improvement of the anatomical area concerned over time, the various degrees of correction obtained for wrinkles, and in general for texture, were objectively evaluated by using a Numeric Rating scale (NRS) 10–0, a modified Vancouver scale and a Berardesca scale. Results: The Berardesca, NRS and Modified Vancouver scales showed that with this technique it was possible to obtain excellent results both when the suspension was injected into wrinkles with the linear retrograde technique, and when it was injected with the micropomphs technique to correct furrows, with the intent to revitalize the tissue through progenitors with adult stemness markers. Conclusion: The combination of microfragmented and microfiltered adipose tissue and cross-linked hyaluronic acid at 50 microns is safe new method to treat soft tissue defects such as deep wrinkles.

Exosomes and Signaling Nanovesicles from the Nanofiltration of Preconditioned Adipose Tissue with Skin-B® in Tissue Regeneration and Antiaging: A Clinical Study and Case Report

“…The isolation protocol is based on MACS technology, which enables the rapid isolation of high-purity and high-yield EVs. Through this procedure, it was verified that, despite the final filtration procedure at 0.2 microns (200 nanometers), there were still particles covering part of the characterization signal, but we could see the presence of numerous vesicles using exosomal marker CD81, typical of regenerative functions [51], and the mesenchymal/endothelial marker CD146, specific for ADSCa after stabilization [52] (Figure 4). This ultrafiltration technique made it possible to obtain microvesicles that did not contain almost any of the components of the interfering adipose disaggregate [27,38].…”

The Role and Prospects of Mesenchymal Stem Cells in Skin Repair and Regeneration

“…EQ-9 potentially inhibits inflammation through increasing the fibroblast survival rate and decreasing intracellular ROS levels [93]. It has been reported that microfragmented adipose tissue containing adipose stem cells combined with a crosslinked hyaluronic acid scaffold can improve soft tissue defects such as deep wrinkles [94].…”

Stem Cells in Facial Regenerative Surgery: Current Clinical Applications. A Multidisciplinary, Systematic Review

“…AD-MSCs have shown a multitude of possible applications in clinical research, ranging from liver injury, myocardium and bone reconstruction, nerve regeneration to muscle-skeletal dysfunctions or diabetes [12].…”