ADIPOR1 regulates genes involved in milk fat metabolism in goat mammary epithelial cells

Zhao, Wangsheng; Adjei, Michael; Wang, Hongmei; Yangliu, Yueling; Zhu, Jiangjiang; Wu, Huijuan

doi:10.1016/j.rvsc.2021.04.001

Cited by 3 publications

(1 citation statement)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Quantitative PCR was used to detect the relative mRNA expression levels with TB Green® Premix Ex Taq™ II (Tli RNaseH Plus) (Takara Biotechnology Co., Ltd., Dalian, China). The 2 −ΔΔCt technique was used to calculate the relative gene expressions [20][21][22].…”

Section: Quantitative Real-time Pcr (Qpcr)mentioning

confidence: 99%

Sulforaphane reduces adipose tissue fibrosis via promoting M2 macrophages polarization in HFD fed-mice

Song

Zhang

Chen

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Background/Objectives: Adipose tissue fibrosis has been identified as a novel contributor to the pathomechanism of obesity associated metabolic disorders. Sulforaphane (SFN) has been shown to have an anti-obesity effect. However, the impact of SFN on adipose tissue fibrosis is still not well understood. Methods: In this study, obese mice induced by high-fat diets (HFD) were used, and SFN was administered through subcutaneous injection to examined the effects on adipose tissue fibrosis. Analysis included quantification of: (i) body weight, food intake, fat mass, glucose tolerance and insulin tolerance; (ii) the relative mRNA and protein levels of fibrosis, inflammation and macrophages polarization related genes; (iii) tissue histology using Hematoxylin-Eosin (H&E), immunohistochemistry and immunofluorescent staining; and (iv) the levels of inflammatory cytokinesin serum. Results: According to the current findings, SFN dramatically enhanced glucose tolerance and decreased body weight in diet-induced-obesity (DIO) mice. Additionally, SFN therapy significantly reduced extracellular matrix (ECM) deposition and altered the expression of genes related to fibrosis. Furthermore, SFN also reduced inflammation and promoted macrophages polarization towards to M2 phenotype in adipose tissue, which protected adipose tissue from fibrosis. Notably, SFN-mediated nuclear factor E2-related factor 2 (Nrf2) activation was crucial in decreasing adipose tissue fibrosis. Conclusions: These results implied that SFN had favorable benefits in the management of adipose tissue fibrosis, which consequently ameliorates obesity-related metabolic problems. Our research provides new treatment strategies for obesity.

show abstract

Section: Quantitative Real-time Pcr (Qpcr)mentioning

confidence: 99%

Sulforaphane reduces adipose tissue fibrosis via promoting M2 macrophages polarization in HFD fed-mice

Song

Zhang

Chen

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

Sulforaphane reduces adipose tissue fibrosis via promoting M2 macrophages polarization in HFD fed-mice

Zhang,

Chen,

Pan

et al. 2024

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

View full text Add to dashboard Cite

Malonyl/Acetyltransferase (MAT) Knockout Decreases Triacylglycerol and Medium-Chain Fatty Acid Contents in Goat Mammary Epithelial Cells

Yao

Luo

Tian

et al. 2022

Foods

View full text Add to dashboard Cite

Malonyl/acetyltransferase (MAT) is a crucial functional domain of fatty acid synthase (FASN), which plays a vital role in the de novo synthesis of fatty acids in vivo. Milk fatty acids are secreted by mammary epithelial cells. Mammary epithelial cells are the units of mammary gland development and function, and it is a common model for the study of mammary gland tissue development and lactation. This study aimed to investigate the effects of MAT deletion on the synthesis of triacylglycerol and medium-chain fatty acids. The MAT domain was knocked out by CRISPR/Cas9 in the goat mammary epithelial cells (GMECs), and in MAT knockout GMECs, the mRNA level of FASN was decreased by approximately 91.19% and the protein level decreased by 51.83%. The results showed that MAT deletion downregulated the contents of triacylglycerol and medium-chain fatty acids (p < 0.05) and increased the content of acetyl-Coenzyme A (acetyl-CoA) (p < 0.001). Explicit deletion of MAT resulted in significant drop of FASN, which resulted in downregulation of LPL, GPAM, DGAT2, PLIN2, XDH, ATGL, LXRα, and PPARγ genes in GMECs (p < 0.05). Meanwhile, mRNA expression levels of ACC, FASN, DGAT2, SREBP1, and LXRα decreased following treatment with acetyl-CoA (p < 0.05). Our data reveals that FASN plays critical roles in the synthesis of medium-chain fatty acids and triacylglycerol in GMECs.

show abstract

ADIPOR1 regulates genes involved in milk fat metabolism in goat mammary epithelial cells

Cited by 3 publications

References 46 publications

Sulforaphane reduces adipose tissue fibrosis via promoting M2 macrophages polarization in HFD fed-mice

Sulforaphane reduces adipose tissue fibrosis via promoting M2 macrophages polarization in HFD fed-mice

Sulforaphane reduces adipose tissue fibrosis via promoting M2 macrophages polarization in HFD fed-mice

Malonyl/Acetyltransferase (MAT) Knockout Decreases Triacylglycerol and Medium-Chain Fatty Acid Contents in Goat Mammary Epithelial Cells

Contact Info

Product

Resources

About