Adiponectin stimulates glucose uptake in mouse blastocysts and embryonic carcinoma cells

Burkuš, Ján; Santos, Anne Navarrete; Schindler, Maria; Babeľová, Janka; Jung, Juliane‐Susanne; Špirková, Alexandra; Kšiňanová, Martina; Kovaříková, Veronika; Fischer, Bernd; Koppel, Juraj; Fabian, Denis; Číkoš, Štefan

doi:10.1530/rep-19-0251

Cited by 4 publications

(3 citation statements)

References 52 publications

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“…For instance, adiponectin affects glucose homeostasis in placental tissues by inhibiting GLUT1 expression in early pregnancy in human chorionic villi [38]. In mouse embryos and embryonal carcinoma cells, adiponectin may stimulate glucose uptake through the action of GLUT4 [39]. In skeletal muscle cells, globular adiponectin increases GLUT4 translocation and glucose uptake but decreases glycogen synthesis [40].…”

Section: Discussionmentioning

confidence: 99%

Regulation and Role of Adiponectin Secretion in Rat Ovarian Granulosa Cells

Zhou,

Zhang,

Jia

et al. 2024

IJMS

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Adiponectin is an important adipokine involved in glucose and lipid metabolism, but its secretion and potential role in regulating glucose utilization during ovarian development remains unclear. This study aims to investigate the mechanism and effects of follicle-stimulating hormones (FSHs) on adiponectin secretion and its following impact on glucose transport in the granulosa cells of rat ovaries. A range of experimental techniques were utilized to test our research, including immunoblotting, immunohistochemistry, immunofluorescence, ELISA, histological staining, real-time quantitative PCR, and transcriptome analysis. The immunohistochemistry results indicated that adiponectin was primarily located in the granulosa cells of rat ovaries. In primary granulosa cells cultured in vitro, both Western blot and immunofluorescence assays demonstrated that FSH significantly induced adiponectin secretion within 2 h of incubation, primarily via the PKA signaling pathway rather than the PI3K/AKT pathway. Concurrently, the addition of the AdipoR1/AdipoR2 dual agonist AdipoRon to the culture medium significantly stimulated the protein expression of GLUT1 in rat granulosa cells, resulting in enhanced glucose absorption. Consistent with these in vitro findings, rats injected with eCG (which shares structural and functional similarities with FSH) exhibited significantly increased adiponectin levels in both the ovaries and blood. Moreover, there was a notable elevation in mRNA and protein levels of AdipoRs and GLUTs following eCG administration. Transcriptomic analysis further revealed a positive correlation between the expression of the intraovarian adiponectin system and glucose transporter. The present study represents a novel investigation, demonstrating that FSH stimulates adiponectin secretion in ovarian granulosa cells through the PKA signaling pathway. This mechanism potentially influences glucose transport (GLUT1) and utilization within the ovaries.

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Section: Discussionmentioning

confidence: 99%

Regulation and Role of Adiponectin Secretion in Rat Ovarian Granulosa Cells

Zhou,

Zhang,

Jia

et al. 2024

IJMS

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show abstract

“…For instance, adiponectin affects glucose homeostasis in placental tissues by inhibiting GLUT1 expression in early pregnancy human chorionic villi (44). In mouse embryos and embryonal carcinoma cells, adiponectin may stimulate glucose uptake through the action of GLUT4 (45). In skeletal muscle cells, globular adiponectin increases GLUT4 translocation and glucose uptake but decreases glycogen synthesis (46).…”

Section: Discussionmentioning

confidence: 99%

A novel role of FSH on regulating ovarian adiponectin expression in rat ovarian granulosa cells

Zhou,

Zhang,

Jia

et al. 2023

Preprint

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Background: Adiponectin, also known as Acrp30, GBP28, or apM1, plays a crucial role in regulating glucose levels, lipid metabolism and insulin sensitivity in various tissues and organs. Despite its broad physiological relevance in female reproduction, its role in ovarian adiponectin secretion and energy utilization remains unknown. Methods: In the present investigation, we employed a suite of sophisticated molecular and cellular techniques to elucidate the expression of lipocalin system components and glucose transporter proteins within the ovarian tissue of rats. Specifically, cellular immunofluorescence, RT-PCR, Western blotting, and immunohistochemistry, alongside ELISA, were meticulously applied for the identification of these proteins. Furthermore, granulosa cells, harvested from rat ovaries, were subjected to an in vitro culture protocol to establish a primary cell culture. Results: In our study, we utilized primary cultured rat granulosa cells to demonstrate for the first time that FSH quickly stimulated adiponectin protein expression in granulosa cells, particularly through the PKA signaling pathway. Consistently, further in vivo study indicated that eCG administration significantly increased adiponectin protein levels in rat ovaries. The subsequent ovarian transcriptome and RT-PCR analysis showed a positive correlation between the expression of intraovarian glucose transporters (Slc2a1, Slc2a3, and Slc2a4) and the adiponectin system (Adipoq, Adipor1, and Adipor2), as well as the protein expression patterns. Although eCG administration in rats notably elevated circulating adiponectin levels, it had no significant effects on blood glucose levels. The current findings proved that AdipoRon (an AdipoR agonist) significantly increased the expression of glucose transporters (GLUT1 and GLUT2) proteins levels, enhancing glucose uptake in granulosa cells. Conclusions: These findings indicate that FSH may modulate ovarian glucose transporter protein expression and glucose utilization through the autocrine actions of adiponectin, thereby influencing ovarian development and function.

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“…In this regard, only plasma membrane expression of GLUT4, GLUT8, and GLUT12 is known to be governed by insulin ( Shao and Tian, 2015 ). For the other GLUT isotypes, also other factors, such as other hormones and also ions, can control the translocation ( Rattigan et al, 1991 ; Benomar et al, 2006 ; Martinez et al, 2014 ; Sertié et al, 2014 ; Gil-Iturbe et al, 2019 ; Burkuš et al, 2020 ). There are important species differences with respect to GLUT expression.…”

Section: Introductionmentioning

confidence: 99%

Dynamics of training and acute exercise-induced shifts in muscular glucose transporter (GLUT) 4, 8, and 12 expression in locomotion versus posture muscles in healthy horses

Vidal Moreno de Vega,

Lemmens,

de Meeûs d’Argenteuil

et al. 2023

Front. Physiol.

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Important changes in glucose transporter (GLUT) expression should be expected if the glucose influx plays a pivotal role in fuelling or connecting metabolic pathways that are upregulated in response to exercise. The aim was to assess GLUT4, 8, and 12 dynamics in response to training and acute exercise.Methods: Sixteen untrained Standardbred mares (3-4 year) performed an incremental SET at the start and end of 8 weeks harness training. M. pectoralis (PM) and M. vastus lateralis (VL) muscle biopsies were taken before and after each SET, allowing for comparing rest and acute samples in untrained (UT) and trained (T) condition using Western Blot for GLUT quantification and Image Pro v.10 for Blot analysis. Data were normalized against GAPDH. Basal GLUT-levels of PM versus VL were analysed with the Wilcoxon matched-pairs signed rank test. The effect of acute exercise or training was assessed using the Friedman test with a post hoc Dunn’s.Results: Basal GLUT4 and GLUT12 protein expression were significantly higher in the VL compared to the PM (PGLUT4 = 0.031 and PGLUT12 = 0.002). Training had no effect on basal GLUT4 expression, neither in the VL (p > 0.9999), nor the PM (p > 0.9999). However, acute exercise in trained condition significantly decreased GLUT4 expression in the VL (p = 0.0148). Neither training nor acute exercise significantly changed total GLUT8 protein expression. Training significantly decreased total GLUT12 protein expression in rest biopsies, only visible in the VL (p = 0.0359). This decrease was even more prominent in the VL after acute exercise in trained condition (PVL = 0.0025).Conclusion: The important changes seen in GLUT12 expression downregulation, both in response to training and acute exercise in the horse, the downregulation of GLUT4 expression after acute exercise in trained condition and the lack of differential shifts in GLUT8 expression in any of the studied conditions, questions the importance of glucose as substrate to fuel training and exercise in healthy horses. These findings encourage to further explore alternative fuels for their involvement in equine muscular energetics.

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Adiponectin stimulates glucose uptake in mouse blastocysts and embryonic carcinoma cells

Cited by 4 publications

References 52 publications

Regulation and Role of Adiponectin Secretion in Rat Ovarian Granulosa Cells

Regulation and Role of Adiponectin Secretion in Rat Ovarian Granulosa Cells

A novel role of FSH on regulating ovarian adiponectin expression in rat ovarian granulosa cells

Dynamics of training and acute exercise-induced shifts in muscular glucose transporter (GLUT) 4, 8, and 12 expression in locomotion versus posture muscles in healthy horses

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