Adiponectin attenuates the osteoblastic differentiation of vascular smooth muscle cells through the AMPK/mTOR pathway

Zhan, Jicheng; Wang, Yanjiao; Wang, Yi; Tang, Zhiyong; Tan, Pan; H, Wu; Liu, You‐Shuo

doi:10.1016/j.yexcr.2014.02.016

Cited by 56 publications

(61 citation statements)

References 32 publications

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“…23 Other studies found that AMPK promotes osteogenesis. 24 Hence, the role of AMPK in calcification and osteogenesis is highly controversial, and the contributions of AMPKα subunits to the development of atherosclerotic calcification in vivo remain unknown.…”

mentioning

confidence: 99%

Ablation of Adenosine Monophosphate-Activated Protein Kinase α1 in Vascular Smooth Muscle Cells Promotes Diet-Induced Atherosclerotic Calcification In Vivo

Cai

Ding

Zhang

et al. 2016

Circulation Research

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mentioning

confidence: 99%

Ablation of Adenosine Monophosphate-Activated Protein Kinase α1 in Vascular Smooth Muscle Cells Promotes Diet-Induced Atherosclerotic Calcification In Vivo

Cai

Ding

Zhang

et al. 2016

Circulation Research

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“…It is well accepted that the expression of ALP and osteocalcin is a marker of osteoblastic differentiation. 26,27 Our results revealed that inhibition of miR-204 expression increased ALP activity and osteocalcin expression and promoted osteogenic transdifferentiation of VICs. In contrast, overexpression of miR-204 attenuated osteoblast differentiation of human aortic VICs.…”

Section: Discussionmentioning

confidence: 70%

MicroRNA-204 Targets Runx2 to Attenuate BMP-2-induced Osteoblast Differentiation of Human Aortic Valve Interstitial Cells

Wang

Chen

Deng

et al. 2015

Journal of Cardiovascular Pharmacology

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Osteoblast differentiation of valve interstitial cells (VICs) is a key step in valve calcification, but the molecular mechanisms involved are not fully understood. In this study, we aimed to investigate whether microRNA (miR)-204-regulated VICs differentiation through modulation of runt-related transcription factor 2 (Runx2), a key transcription factor for osteogenesis. Our data demonstrated that miR-204 was markedly downregulated in both human calcified aortic valves and bone morphogenetic protein (BMP)-2-stimulated aortic VICs. In vitro experiments showed that miR-204 acted as a negative regulator of osteogenic differentiation by repressing Runx2 and thereby inhibiting expression of osteoblast-related genes, including alkaline phosphatase and osteocalcin, which were all induced by BMP-2. Luciferase reporter assays validated Runx2 as the direct target of miR-204. Furthermore, increased alkaline phosphatase activity and osteocalcin expression after miR-204 inhibition were abolished by small interfering RNA-mediated silencing of Runx2. Overall, these data suggested miR-204 as a possible molecular switch inhibiting osteoblastic transdifferentiation of human aortic VICs and targeting miR-204 may have therapeutic potential for human aortic valve calcification.

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“…Our previous study demonstrated that the mTOR signaling pathway is involved in osteoblastic differentiation of VSMCs [19,20]. PI3K and Akt are the upstream molecules of mTOR signaling, and S6K1 is a wellknown downstream target of mTOR.…”

Section: Liraglutide Inhibits Osteoblastic Differentiation Of Human Vmentioning

confidence: 99%

“…The mammalian target of rapamycin (mTOR) pathway is an essential cellular signaling pathway involved in a number of important physiological functions [18]. Our recent study showed that the mTOR signaling pathway is involved in osteoblastic differentiation of VSMCs [19,20]. We hypothesized that GLP-1 may attenuate osteoblastic differentiation of VSMCs through the GLP-1R/PI3K/Akt/mTOR pathway.…”

Section: Introductionmentioning

confidence: 99%

The protective effect of GLP-1 analogue in arterial calcification through attenuating osteoblastic differentiation of human VSMCs

Zhan¹,

Wang²,

Wang³

et al. 2015

International Journal of Cardiology

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Adiponectin attenuates the osteoblastic differentiation of vascular smooth muscle cells through the AMPK/mTOR pathway

Cited by 56 publications

References 32 publications

Ablation of Adenosine Monophosphate-Activated Protein Kinase α1 in Vascular Smooth Muscle Cells Promotes Diet-Induced Atherosclerotic Calcification In Vivo

Ablation of Adenosine Monophosphate-Activated Protein Kinase α1 in Vascular Smooth Muscle Cells Promotes Diet-Induced Atherosclerotic Calcification In Vivo

MicroRNA-204 Targets Runx2 to Attenuate BMP-2-induced Osteoblast Differentiation of Human Aortic Valve Interstitial Cells

The protective effect of GLP-1 analogue in arterial calcification through attenuating osteoblastic differentiation of human VSMCs

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