Adiponectin and adiponectin receptor genes are coexpressed during zebrafish embryogenesis and regulated by food deprivation

Nishio, Shintaro; Gibert, Yann; Bernard, Laure; Brunet, Frédéric; Triqueneaux, Gérard; Laudet, Vincent

doi:10.1002/dvdy.21559

Cited by 60 publications

(69 citation statements)

References 39 publications

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“…A reduction in the expression of adiponectin in adipose tissue was found after a fasting period, while the expression of adipoR1 increased in the muscle of mice (Bertile and Raclot, 2004;Tsuchida et al, 2004) and in the liver of zebra fish (Nishio et al, 2008).…”

Section: Introductionmentioning

confidence: 93%

“…Nevertheless, there is little information available about adipokines in lower vertebrates such as fish. Although the presence of leptin and tumour necrosis factor  (TNF) has been described in various fish species (Kling et al, 2009;SaeraVila et al, 2007), adiponectin has been identified in zebra fish (Nishio et al, 2008), and only very recently in rainbow trout (Kondo et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Adiponectin effects and gene expression in rainbow trout: an in vivo and in vitro approach

Sanchez-Gurmaches

Cruz-García

Gutiérrez

et al. 2012

Journal of Experimental Biology

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SUMMARYHere we present the presence of adiponectin and adiponectin receptors [type 1 (adipoR1) and type 2 (adipoR2)] in rainbow trout (Oncorhynchus mykiss) tissues and cell cultures together with the response to different scenarios. In response to fasting, adiponectin expression was up-regulated in adipose tissue, while the expression of its receptors increased in white and red muscle. Insulin injection decreased adipoR1 expression in white and red muscles. We deduce that the adipoRs in trout muscle show opposite responses to increasing insulin plasma levels, which may maintain sensitivity to insulin in this tissue. Adiponectin expression was inhibited by the inflammatory effect of lipopolysaccharide (LPS) in adipose tissue and red muscle. Moreover, results indicate that LPS may lead to mobilization of fat reserves, increasing adipoR1 expression in adipose tissue. The effects of LPS could be mediated through tumour necrosis factor  (TNF), at least in red muscle. Insulin, growth hormone and TNF all diminished expression of adipoR2 in adipocytes and adipoR1 in myotubes, while insulin increased the expression of adipoR2 in the muscle cells. Adiponectin activates Akt in rainbow trout myotubes, which may lead to an increase in fatty acid uptake and oxidation. Overall, our results show that the adiponectin system responds differently to various physiological challenges and that it is hormonally controlled in vivo and in vitro. To the best of our knowledge, this is the first time this has been demonstrated in teleosts, and it may be a valuable contribution to our understanding of adipokines in fish.

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Section: Introductionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Adiponectin effects and gene expression in rainbow trout: an in vivo and in vitro approach

Sanchez-Gurmaches

Cruz-García

Gutiérrez

et al. 2012

Journal of Experimental Biology

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“…Furthermore, adipose tissue in lower exothermic animals is usually small because the liver performs the major lipid-storing function. Interestingly, in these animals, the liver secretes many of the mammalian orthologs of the adipokines, such as adiponectin and leptin (Huising et al, 2006;Nishio et al, 2008;Pfundt et al, 2009).…”

Section: Box 2 Strategies For Tag Synthesismentioning

confidence: 99%

A comparative perspective on lipid storage in animals

Birsoy

Festuccia

Laplante

2013

Journal of Cell Science

125

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SummaryLipid storage is an evolutionary conserved process that exists in all organisms from simple prokaryotes to humans. In Metazoa, longterm lipid accumulation is restricted to specialized cell types, while a dedicated tissue for lipid storage (adipose tissue) exists only in vertebrates. Excessive lipid accumulation is associated with serious health complications including insulin resistance, type 2 diabetes, cardiovascular diseases and cancer. Thus, significant advances have been made over the last decades to dissect out the molecular and cellular mechanisms involved in adipose tissue formation and maintenance. Our current understanding of adipose tissue development comes from in vitro cell culture and mouse models, as well as recent approaches to study lipid storage in genetically tractable lower organisms. This Commentary gives a comparative insight into lipid storage in uni-and multi-cellular organisms with a particular emphasis on vertebrate adipose tissue. We also highlight the molecular mechanisms and nutritional signals that regulate the formation of mammalian adipose tissue.

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“…As predicted, inversion of phtf1 GtN/+ or Felix-ina GtN/+ with Cre or Flp recombinase did not activate mCherry expression because the fluorescent protein is out of frame. Highlighting the increased sensitivity of LM-PCR compared with fluorescent detection, mCherry expression was not detected in adipor2 GtN embryos following inversion even though it was fused in frame, which was likely due to the low level of expression at this locus (12). We analyzed the effects of intronic insertions on host gene mRNA expression by quantitative RT-PCR (qRT-PCR) (primers listed in Table S1).…”

Section: Resultsmentioning

confidence: 99%

Conditional control of gene function by an invertible gene trap in zebrafish

Ni¹,

Zhu³

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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Conditional mutations are essential for determining the stage-and tissue-specific functions of genes. Here we achieve conditional mutagenesis in zebrafish using FT1, a gene-trap cassette that can be stably inverted by both Cre and Flp recombinases. We demonstrate that intronic insertions in the gene-trapping orientation severely disrupt the expression of the host gene, whereas intronic insertions in the neutral orientation do not significantly affect host gene expression. Cre-and Flp-mediated recombination switches the orientation of the gene-trap cassette, permitting conditional rescue in one orientation and conditional knockout in the other. To illustrate the utility of this system we analyzed the functional consequence of intronic FT1 insertion in supv3l1, a gene encoding a mitochondrial RNA helicase. Global supv311 mutants have impaired mitochondrial function, embryonic lethality, and agenesis of the liver. Conditional rescue of supv311 expression in hepatocytes specifically corrected the liver defects. To test whether the liver function of supv311 is required for viability we used Flp-mediated recombination in the germline to generate a neutral allele at the locus. Subsequently, tissue-specific expression of Cre conditionally inactivated the targeted locus. Hepatocyte-specific inactivation of supv311 caused liver degeneration, growth retardation, and juvenile lethality, a phenotype that was less severe than the global disruption of supv311. Thus, supv311 is required in multiple tissues for organismal viability. Our mutagenesis approach is very efficient and could be used to generate conditional alleles throughout the zebrafish genome. Furthermore, because FT1 is based on the promiscuous Tol2 transposon, it should be applicable to many organisms.H igh throughput functional genomic and informatic methods have been developed to interrogate the genome and extract functional predictions about many genes at a time. However, careful phenotypic analysis of genetic mutants remains the sine qua non of reductionist biological science. In most experimental organisms, random mutagenesis is the preferred or only mutagenic technique available. DNA alkylating agents, transposable elements, or retroviruses are traditionally used in these organisms. A major limitation of these traditional genetic methods is that they reveal only the earliest and/or most prominent function of a gene as later functions are masked by the earlier phenotype, which is often lethality. To assess later functions, for example in metabolism, aging, or behavior, conditional alleles are required.The development of conditional alleles has proven a boon to studying gene function in temporally or spatially restricted contexts. Traditional conditional alleles disrupt gene function by changing the environment, for example by increasing the temperature. Engineered conditional alleles disrupt gene function by activating a recombination-mediated molecular switch that ablates gene function in one state, but has no functional consequences in the other state (1, 2...

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Adiponectin and adiponectin receptor genes are coexpressed during zebrafish embryogenesis and regulated by food deprivation

Cited by 60 publications

References 39 publications

Adiponectin effects and gene expression in rainbow trout: an in vivo and in vitro approach

Adiponectin effects and gene expression in rainbow trout: an in vivo and in vitro approach

A comparative perspective on lipid storage in animals

Conditional control of gene function by an invertible gene trap in zebrafish

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