Adiponectin action in skeletal muscle

Liu, Ying; Sweeney, Gary

doi:10.1016/j.beem.2013.08.003

Cited by 93 publications

(72 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Adiponectin has been proposed to be a cytokine with protective properties, but surrounded by controversy 17,33,34 . Recently, it has emerged an interestingly facet showing that adiponectin is also a myokine, being produced and released by skeletal muscle 10 .…”

Section: Discussionmentioning

confidence: 99%

“…Adiponectin concentrations have been negatively associated with insulin resistance 5 , CRP 6 and adiposity 7 , positively correlated with HDL-cholesterol 6,8 and it is considered an independent CVD risk factor 9 . It was originally assumed that adiponectin was exclusively synthesized and expressed by adipocytes 10 . However, recently it was suggested that adiponectin is also secreted and expressed by cardiomyocytes, bone-forming cells, pituitary cells and skeletal muscle 10 . Likewise, research has begun to demonstrate that high levels of adiponectin may play a paradoxical role in the pathogenesis of CVD.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Association between serum adiponectin levels and muscular fitness in Portuguese adolescents: LabMed Physical Activity Study

Agostinis‐Sobrinho

Santos

Moreira

et al. 2016

Nutrition, Metabolism and Cardiovascular Diseases

View full text Add to dashboard Cite

Background and aim: Paradoxically, recent investigations have showed that adiponectin levels are inversely associated with muscle strength. However, to date, there is a lack of knowledge on the relationship between muscular fitness (MF) and adiponectin levels in adolescents. We aimed to examine the independent associations between MF and adiponectin levels in adolescents, controlling for several potential confounders. Methods and results: This is a cross-sectional analysis with 529 Portuguese adolescents aged 12-18 years. A MF score was computed as the mean of the handgrip strength and standing long jump standardized values by age and gender. We measured fasting glucose, insulin, HDL-cholesterol, C-reactive protein and adiponectin. Linear regression analysis showed a significant inverse association between adiponectin (Z-score by age and sex) and MF score, after adjustments for age, sex, pubertal stage, socioeconomic status, adherence to the Mediterranean diet, body mass index, HOMA-IR, HDL-cholesterol, C-reactive protein and cardiorespiratory fitness (unstandardized β = -0.176; p < 0.005). Analysis of covariance showed a significant difference between the Low MF/Non-overweight group and the High MF/Non-overweight Group (p < 0.05) and between the Low MF/Non-overweight and High MF/Overweight Group (p < 0.05) (F (5, 523) = 2.262, p = 0.047). Conclusion: Adiponectin circulating levels are inversely and independently associated with MF. In nonoverweight adolescents, those with high levels of MF presented lower levels of adiponectin compared to those with Low MF. Likewise, overweight adolescents with High MF presented lower levels of serum adiponectin than non-overweight adolescents with Low MF.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Association between serum adiponectin levels and muscular fitness in Portuguese adolescents: LabMed Physical Activity Study

Agostinis‐Sobrinho

Santos

Moreira

et al. 2016

Nutrition, Metabolism and Cardiovascular Diseases

View full text Add to dashboard Cite

show abstract

“…The association of adiponectin with exercise has recently been more frequently examined [12][13][14] because of its action in muscle 11,21) . Thus far, however, there has been no consensus as to the effect of exercise on serum adiponectin concentration, which has been reported to decrease 22,23) , increase 5,6,24) , and remain unchanged 7,25) with exercise.…”

Section: Conflict Of Interestsmentioning

confidence: 99%

“…Adiponectin, an adipocytokine, favorably influences the development of atherosclerosis and energy homeostasis 8) , and has received much attention in relation to the metabolic effects of exercise, partly due to the effects of adiponectin on the modulation of skeletal muscle energy metabolism in animal models [9][10][11] . In fact, studies have revealed that long-term exercise that improves fitness levels, increases insulin sensitivity, and reduces body fat, is also associated with increased resting adiponectin levels [12][13][14] .…”

Section: Introductionmentioning

confidence: 99%

Decrease in regional body fat after long-term high-intensity interval training

Azuma

Osawa

Tabata

et al. 2017

JPFSM

View full text Add to dashboard Cite

High-intensity interval training (HIIT) has recently received much attention as a new option for aerobic training. Despite its smaller time requirement, HIIT has been reported to have a greater effect than continuous moderate-intensity training on fat loss, especially a decrease in truncal adiposity. We therefore examined whether long-term HIIT preferentially modulates truncal adiposity rather than peripheral adiposity, especially thigh adiposity, where local muscle energy consumption increased profoundly during HIIT. We also examined the association between changes in adipose tissue distribution and serum adiponectin level. Twelve healthy male participants (28-48 years old) were assigned to a group that performed HIIT using only a leg ergometer (L-HIIT, n = 7) or to a group that performed HIIT using both leg and arm ergometers (LA-HIIT, n = 5) twice weekly for 16 weeks. The training programs consisted of 8 to 12 sets of >90% V ・ O 2 peak for 1 min, with 1 min of very light active recovery. Body composition analyses as well as aerobic fitness and measurements of serum adiponectin were performed at baseline and after intervention. A linear improvement in aerobic fitness was observed along with a decrease in leg fat (5.4 ± 1.7 vs. 5.1 ± 1.7 kg, p < 0.05) near the main working muscles during HIIT in the combined (L+LA-HIIT) group. Moreover, there was an association of decrease in leg fat or thigh adiposity with improvement in aerobic fitness in the combined group (ρ = -0.59, p < 0.05; and ρ = -0.71, p < 0.05, respectively). Visceral adiposity was decreased in L-HIIT (115 ± 45 vs. 100 ± 47 cm 2 , p < 0.05), however no decrease was observed in total fat or truncal fat in either group. No change was observed in serum adiponectin concentration in either group. Changes in serum adiponectin were associated with changes in visceral adiposity in the combined group (ρ = -0.72, p < 0.01). Regional rather than whole-body fat loss was observed after a 16-week HIIT program.

show abstract

“…Hence, therapeutic strategies to reverse hypoadiponectinaemia are attractive. Increasing evidence indicates that adiponectin resistance also contributes to the development of metabolic and cardiovascular diseases 42,43,45,46,208,[234][235][236][237] . While the molecular mechanisms that give rise to adiponectin resistance are poorly defined strategies to overcome adiponectin resistance are also of therapeutic potential.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterisation of the adiponectin receptors, AdipoR1 and AdipoR2

Keshvari¹

View full text Add to dashboard Cite

The increasing global prevalence of cardiovascular and metabolic diseases necessitates the development of more effective therapeutic strategies that, in turn, require a greater understanding of the regulatory networks involved. Research over the last decade has increased our appreciation of the key role of the adiponectin axis as a major regulator of metabolic, cardiovascular and inflammatory tone, thereby establishing it as a province of therapeutic opportunity. The receptors for adiponectin, AdipoR1 and AdipoR2, are distant relatives of the largest single class of drug targets, the G-protein coupled receptor (GPCR) family. However, unlike GPCRs they have intracellular N-termini and extracellular C-termini and signal via atypical pathways. Our current understanding of AdipoR1 and AdipoR2 is rudimentary, constraining our ability to target these receptors effectively. The aim of this thesis was to characterise molecular features of AdipoR1 and AdipoR2 that facilitate adiponectin signal transduction to advance our understanding and identify strategies to enhance adiponectin's beneficial effects.We have begun to characterise basic properties of AdipoR1 and AdipoR2, focusing on molecular factors that drive cell-surface expression (CSE) of the receptors using a range of C-terminal, epitope-tagged AdipoR1 and AdipoR2 constructs. Surprisingly, under steady-state conditions (no serum starvation) only AdipoR1 was readily detected on the cell-surface (cell-surface ratio of AdipoR1 vs AdipoR2 is 0.6±0.1 vs 0.15±0.1, p<0.05). Generation and characterisation of a series of chimeric and truncated constructs demonstrated that a non-conserved, intracellular, N-terminal region of AdipoR2 (R2 ) restricted its CSE whilst the same region in AdipoR1 (R1 (1-70) ) promoted its CSE. We also confirmed that AdipoR1 and AdipoR2 form heterodimer and that coexpression of these receptors increase the CSE of AdipoR2. Subsequently, we provided evidence that the subcellular localisation of AdipoR1 and AdipoR2 is governed by multiple motifs across their non-conserved and conserved cytoplasmic domains. For instance, two highly conserved motifs, an ER exit motif (FxxxFxxxF) and Di-Leucine motif (DxxxLL), in the conserved Nterminal domain are required for the proper CSE of both AdipoR1 and AdipoR2, whilst different parts of the non-conserved domain of AdipoR2 inhibits its CSE.Moreover, we demonstrated that in HEK-293 cells over-expressing AdipoR1 adiponectin activated downstream signalling networks (AMPK, AKT, ERK & P38MAPK) acutely (peaking at 15 min) whereas signal transduction via AdipoR2 was relatively chronic (peaking at 24 h). This difference was also underpinned by the non-conserved N-terminal domains of AdipoR1 and AdipoR2. We also demonstrated that a number of conserved and non-conserved cysteines in the N-terminal domain of iii AdipoR1 and AdipoR2 are subject to palmitoylation and that palmitoylation of a conserved cysteine, situated in the juxta-membrane region of the N-termini of AdipoR1 and AdipoR2 in a position analogous to t...

show abstract

Adiponectin action in skeletal muscle

Cited by 93 publications

References 75 publications

Association between serum adiponectin levels and muscular fitness in Portuguese adolescents: LabMed Physical Activity Study

Association between serum adiponectin levels and muscular fitness in Portuguese adolescents: LabMed Physical Activity Study

Decrease in regional body fat after long-term high-intensity interval training

Molecular characterisation of the adiponectin receptors, AdipoR1 and AdipoR2

Contact Info

Product

Resources

About