Adipokines expression in reproductive tract, egg white and embryonic annexes in hen

Expression of chemerin and its receptors in extra-embryonic annexes and role of chemerin and its GPR1 receptor in embryo development in layer and broiler hens

“…Chemerin concentrations were measured from amniotic and allantoic fluids and plasma of eggs incubated by using enzyme-linked immunosorbent assays ( ELISA ) as previously described by Bernardi et al (2022 , 2023 ). The recombinant chicken chemerin protein (full length, rRARRES2) was obtained from the Gallus gallus sequence (NM_001277476.1), produced in Escherichia coli and purified by a chromatography column-based on His-Tag under denaturing conditions (Agro-Bio, La Ferté Saint Aubin, France).…”

“…Proteomic analyzes have also identified the presence of chemerin in the egg white and perivitelline membranes of chicken eggs ( Mann, 2007 , 2008 ). We previously showed that chemerin in egg white comes from a part of oviduct named magnum ( Bernardi et al, 2022 , 2023 ; Estienne et al, 2022 ). During the early embryo development, chemerin system is also present in extraembryonic annexes such as allantoic and amniotic membranes and fluids, suggesting a passage of chemerin from the egg white to the embryo annexes and more specifically in the amniotic fluid ( Geelhoed and Conklin, 1966 ; Yoshizaki et al, 2002 ; Estienne et al, 2022 ).…”