Adipokine and Cytokine Profiles of Epicardial and Subcutaneous Adipose Tissue in Patients with Coronary Heart Disease

Груздева, О. В.; Акбашева, О. Е.; Dyleva, Yulia; Антонова, Л. В.; Матвеева, В. Г.; Uchasova, Evgenya; Fanaskova, E. V.; Каретникова, В. Н.; Иванов, Сергей Витальевич; Barbarash, O. L.

doi:10.1007/s10517-017-3860-5

Cited by 69 publications

(47 citation statements)

References 12 publications

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“…По данным этой группы исследователей уровень лептина был выше в адипоцитах ЭЖТ, чем ПЖТ. Однако экспрессию гена лептина в различных видах жировой ткани эти авторы не изучали [46]. На основании изучения секреции лептина, ФНО-a, ИЛ-1, адипонектина, ИЛ-10 и FGF-b в культурах адипоцитов ЭЖТ и ПЖТ авторами было высказано предположение о непосредственном вовлечении эпикардиальных жировых клеток в патогенез ИБС за счет формирования адипокинового дисбаланса и активации провоспалительных реакций [46].…”

Section: Discussionunclassified

Subcutaneous and Epicardial Adipose Tissue Leptin Gene Expression in Coronary Artery Disease Patient

Полякова

Колодина

Miroshnikova

et al. 2019

Transl. med. (Print)

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Objective. To assess the expression of the leptin gene (LEP) in the epicardial (EAT) and subcutaneous (SAT) adipose tissue in coronary artery disease (CAD) patients.Methods. 107 people were examined: 87 with CAD (57 men, 30 women), 20 without CAD (10 men, 10 women). Biopsy of EAT, SAT, coronary angiography, heart computed tomography, blood leptin levels were estimate, mRNA expression of the LEP gene evaluated by PCR.Results. In CAD patients with multivessel coronary artery lesion, the level of blood leptin is the highest. The expression of the LEP gene in EAT is higher in men than in women, in men with CAD higher than in non-coronary artery disease patients, and with a multivascular coronary lesion higher than in a 1–2 vascular lesion. Expression of the LEP gene in EAT in women with CAD is higher than with no CAD, and does not depend on the severity of coronary atherosclerosis. In SAT, LEP gene expression in men with CAD is higher than without CAD; women with CAD are lower than without CAD. Expression of the LEP gene in SAT is higher in men with a multivascular lesion than in 1–2 vascular lesion, and lower in women with a multivascular damage.Conclusions. The expression of the leptin gene in women is higher in SAT, and in men in EAT. Expression of the leptin gene in EAT with CAD is higher than without CAD. The expression of the leptin gene in SAT in men with CAD, especially with multivessel lesions, is higher than without coronary artery disease.

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Section: Discussionunclassified

Subcutaneous and Epicardial Adipose Tissue Leptin Gene Expression in Coronary Artery Disease Patient

Полякова

Колодина

Miroshnikova

et al. 2019

Transl. med. (Print)

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“…Существенную роль в фиброзировании миокарда играет эпикардиальная жировая ткань (ЭЖТ), расположенная в анатомической близости к миокарду и способная секретировать факторы фиброгенеза: провоспалительные цитокины и адипокины [4]. Адипокины и провоспалительные цитокины, выделяемые жировой тканью (ЖТ), способны влиять на метаболические процессы миокарда, усиливать гипертрофию кардиомиоцитов и гибель клеток, потенцировать изменения структуры и состава внеклеточного матрикса [5].…”

Section: Predictors Of Myocardial Fibrosis and Loss Of Epicardial Adiunclassified

Predictors of myocardial fibrosis and loss of epicardial adipose tissue volume in the long-term period after myocardial infarction

et al. 2020

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Aim. To assess the changes of biochemical markers in hospitalization, the relationship with the severity of myocardial fibrosis and the epicardial adipose tissue (EAT) thickness one year after myocardial infarction (MI).Material and methods. A total of 88 patients (65 men and 23 women) with MI were examined. The percentage of cicatricial changes in the myocardium and the EAT thickness were measured using the magnetic resonance imaging (MRI) one year after MI. In the hospitalization (days 1 and 12) and 1 year after MI, the concentrations of N-terminal pro-brain natriuretic peptide (NT-proBNP), stimulating growth factor (ST2), interleukin-33 (IL-33) and type I collagen (COL-1). The data were analyzed using descriptive statistics, correlation and ROC analysis, and logistic regression (Statistica 9.0).Results. One year after MI, cicatricial changes were detected in 68 (77%) patients: 27 people had myocardial fibrosis <5%, 22 patients — 5-15%, and 19 patients >15%. We established that myocardial fibrosis after MI is associated with unfavorable medical history, a complicated course during in-hospital period and higher concentrations of ST2, NT-proBNP, COL-1 compared with patients without myocardial fibrosis. High levels of ST2, NT-proBNP increase the risk of myocardial fibrosis by 1,2 and 1,8 times after hospitalization, respectively. In patients with myocardial fibrosis >15%, IL-33 level was significantly lower in the 1st day of MI. It was found that the EAT thickness increases with fibrosis of 5-15%. An increase in the left (LV) and right ventricular (RV) EAT thickness by 1,33 times and 1,34 times, respectively, increases the risk of myocardial fibrosis (LV EAT thickness, mm (OR 1,33; 95% CI (1,08-1,4), AUC 0,75; RV EAT thickness, mm (OR 1,34; 95% CI (1,15-1,43), AUC 0,79). In patients with myocardial fibrosis >15%, EAT thickness decreases and correlates with NT-proBNP increase in the acute period and a one year after MI.Conclusion. The development of myocardial fibrosis one year after MI is associated with an increase in ST2, NT-proBNP, COL-1, both in the hospitalization and 1 year after MI. The decrease in IL-33 concentration during hospitalization with MI is accompanied by the development of fibrosis >15% of the myocardium.

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“…Adipocytes were isolated from ATs under sterile conditions in a laminar ow hood (BOV-001-AMS MZMO, Millerovo, Russia) using the method described earlier [6]. Adipocytes were counted in a Goryaev's chamber.…”

Section: Cells Collectionmentioning

confidence: 99%

Expression of adipocytokine genes and their content in various adipose tissue sites in patients with heart diseases

Груздева¹,

Dyleva

Белик

et al. 2020

Preprint

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Background Adipose tissue (AT) is an endocrine and paracrine organ that synthesizes biologically active adipocytokines, which affect inflammation, fibrosis, and atherogenesis. Epicardial and perivascular fat depots are of great interest owing to potential effects on the myocardium and blood vessels. Objective To assess expression and secretion of adipocytokine genes in adipose tissue in patients coronary artery disease (CAD) and patients with aortic or mitral valve replacement. Methods The study included 84 patients with CAD and 50 patients with aortic or mitral valve replacement. Adipocytes were isolated from subcutaneous (SAT), epicardial (EAT), and perivascular AT (PVAT) samples. Isolated adipocytes were cultured for 24 h after which, gene expression and secretion levels of selected adipokines and cytokines in the culture medium were determined. Results The study parameters differed depending on the adipose tissue location. EAT adipocytes in CAD patients were characterized by a pronounced imbalance in the adipokine system. EAT had the lowest adiponectin gene expression and secretion, regardless of nosology and high expression levels of the leptin gene, its receptor, and interleukin-6 (IL-6) were detected. High leptin and IL-6 levels resulted in increased pro-inflammatory activity, as observed in both EAT and PVAT adipocytes, especially in individuals with coronary artery disease. Conclusion The "protective" potential of adipose tissue depends on its location.

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Adipokine and Cytokine Profiles of Epicardial and Subcutaneous Adipose Tissue in Patients with Coronary Heart Disease

Cited by 69 publications

References 12 publications

Subcutaneous and Epicardial Adipose Tissue Leptin Gene Expression in Coronary Artery Disease Patient

Subcutaneous and Epicardial Adipose Tissue Leptin Gene Expression in Coronary Artery Disease Patient

Predictors of myocardial fibrosis and loss of epicardial adipose tissue volume in the long-term period after myocardial infarction

Expression of adipocytokine genes and their content in various adipose tissue sites in patients with heart diseases

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