Adipogenic, osteogenic and myofibrogenic differentiations of a rat malignant fibrous histiocytoma (MFH)-derived cell line, and a relationship of MFH cells with embryonal mesenchymal, perivascular and bone marrow stem cells

Immunohistochemical characterization of glial fibrillary acidic protein (GFAP)-expressing cells in a rat liver cirrhosis model induced by repeated injections of thioacetamide (TAA)

“…It is reported that hepatic myofibroblasts may originate partly from bone marrow-derived stem cells (Russo et al, 2006). A3-expressing cells have been regarded as a stromal stem cell originating from the bone marrow, although the molecular properties of A3-recognizing antigens remain to be elucidated (Yamate et al, 2007). These findings suggest that a small population of GFAP-expressing myofibroblasts may be derived from mesenchymal or bone marrow stem cells in the TAA cirrhosis rat model.…”

“…To investigate the possible derivation of GFAP-positive cells from stem cells, we used neural stem cell marker antibody nestin and mesenchymal stem cell marker antibodies Thy-1 and A3 (Bae et al, 2008;Namiki et al, 2012;Yamate et al, 2007). There were no positive cells reacting to nestin in control livers.…”

Transformation potential of bone marrow stromal cells into undifferentiated high-grade pleomorphic sarcoma

“…In rat BM tissues, some cells were stained positively with a rat MFH cell-speciWc antibody (A3). From these Wndings, Yamate et al suggested that progenitors of MFH might be involved in the lineage of BM stem cells capable of diVerentiating into mesenchymal cells (Yamate et al 2007). Thus, both Yamate et al and we have arrived at a similar conclusion, albeit by diVerent experimental routes.…”

Participation of bone morphogenetic protein (BMP)-6 and osteopontin in cisplatin (CDDP)-induced rat renal fibrosis

“…However, detailed roles of BMP-6 and OPN have not yet been decided. To know their roles in renal fibrogenesis, we investigated their expression patterns in cisplatin (CDDP; cis-diamminedichloroplatinum II)-induced renal fibrosis in rats in vivo (Yamamoto et al, 2012) of BMP-6 and OPN in modulating fibrogenic factors was analyzed by using kidney-constituting cell lines in vitro: renal interstitial fibroblastic cell line (NRK-49F), renal tubular epithelial cell line (NRK-52E), and immature mesenchymal cell line (MT-9) resembling pericytes (Yamate et al, 2007). The present study shows the participation of BMP-6 and OPN in the CDDP-induced renal fibrosis, in close association with TGF-␤1.…”