Adipogenic differentiation of laser-printed 3D tissue grafts consisting of human adipose-derived stem cells

Gruene, Martin; Pflaum, Michael; Deiwick, Andrea; Koch, Lothar; Schlie, Sabrina; Unger, Claudia; Wilhelmi, Michaela; Haverich, Axel; Chichkov, Boris N.

doi:10.1088/1758-5082/3/1/015005

Cited by 142 publications

(99 citation statements)

References 42 publications

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“…Additionally, printed grid patterns ( Figure 3) were successfully differentiated over several weeks into bone, fat, and cartilage tissue grafts [3,16], indicating that LaBP is capable of printing cell densities high enough for the promotion of chondrogenesis. 3D scaffold-free tissue grafts were fabricated with LaBP keeping their predefined shape after several weeks in culture, even if removing the matrix material after 2 weeks in culture.…”

Section: Printing Stem Cell Graftsmentioning

confidence: 99%

“…Gruene et al [3,16] printed mesenchymal stem cells [MSCs, porcine bone-marrow derived (pMSCs) and human adipose derived (ASCs)] in monocellular 3D patterns (approximately 300 μm high) to study if these stem cell grafts can be differentiated within the printed pattern and if the printing process affects the stem cells differentiation potential towards different lineages (osteogenic, chondrogenic, and adipogenic). A mixture of alginate and ethylene-diamine-tetra-acetic (EDTA) blood plasma was applied as the sol for printing and cross-linked with calcium chloride to form a firm extracellular matrix.…”

Section: Printing Stem Cell Graftsmentioning

confidence: 99%

“…Since stem cell differentiation can be induced by mechanical forces, the effect of laser-printing on the Brought to you by | MIT Libraries Authenticated Download Date | 5/12/18 5:07 AM phenotype [14] and the differentiation potential [3,16] of mesenchymal stem cells was studied. It was shown that phenotype and differentiation behavior were not influenced.…”

Section: Introductionmentioning

confidence: 99%

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Laser-based 3D cell printing for tissue engineering

2014

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Currently, different 3D printing techniques are investigated for printing biomaterials and living cells. An ambitious aim is the printing of fully functional tissue or organs. Furthermore, for manifold applications in biomedical research and in testing of pharmaceuticals or cosmetics, printed tissue could be a new method, partly substituting test animals. Here we describe a laser-based printing technique applied for the arrangement of vital cells in two and three-dimensional patterns and for tissue engineering. First printed tissue, tested in vitro and in vivo, and printing of cell patterns for investigating cell-cell interactions are presented.

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Section: Printing Stem Cell Graftsmentioning

confidence: 99%

Section: Printing Stem Cell Graftsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Laser-based 3D cell printing for tissue engineering

2014

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“…2 Adipose-derived mesenchymal stem cells Alginate Adipose Laser-assisted (a) Gruene et al 2011 [119] (a) Adipogenic lineage pathway maintained for 10 days with expression of adipogenic markers similar to those expressed in native adipose tissue.…”

Section: Induced Pluripotent Stem Cells (Ipsc)mentioning

confidence: 99%

3D bioprinting for tissue engineering: Stem cells in hydrogels

Mehrban¹,

Teoh²,

Birchall³

2024

IJB

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Surgical limitations require alternative methods of repairing and replacing diseased and damaged tissue. Regenerative medicine is a growing area of research with engineered tissues already being used successfully in patients. However, the demand for such tissues greatly outweighs the supply and a fast and accurate method of production is still required. 3D bioprinting offers precision control as well as the ability to incorporate biological cues and cells directly into the material as it is being fabricated. Having precise control over scaffold morphology and chemistry is a significant step towards controlling cellular behaviour, particularly where undifferentiated cells, i.e., stem cells, are used. This level of control in the early stages of tissue development is crucial in building more complex systems that morphologically and functionally mimic in vivo tissue. Here we review 3D printing hydrogel materials for tissue engineering purposes and the incorporation of cells within them. Hydrogels are ideal materials for cell culture. They are structurally similar to native extracellular matrix, have a high nutrient retention capacity, allow cells to migrate and can be formed under mild conditions. The techniques used to produce these materials, as well as their benefits and limitations, are outlined.

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“…Specifically for LAB applications, the bio-ink should harbor properties similar to the physiological extracellular matrix, which is critical for cell homeostasis in vivo [36,37]. Accordingly, the cells have successfully been printed using various solutions: culture medium alone [22], in combination with sodium alginate [38], thrombine [18], combination of hyaluronic acid and fibrinogen [26] or a combination of blood plasma and sodium alginate [24,39]. Culture medium supplemented with sodium alginate, or hydrogels like Collagen type I and Matrigel™ can be used as well.…”

Section: Bio-ink Compositionmentioning

confidence: 99%

Laser Assisted Bio-printing (LAB) of Cells and Bio-materials Based on Laser Induced Forward Transfer (LIFT)

Guillotin

Catros

Guillemot

2013

Laser Technology in Biomimetics

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Laser assisted bio-printing (LAB) is an emerging and complementary technology in the field of tissue engineering envisaging biomimetics applications. LAB allows to print cells and liquid materials with a cell-level resolution, which is comparable to the complex histology of living tissues. By giving tissue engineers control on cell density and organization, LAB potentially holds promise to fabricate living tissues with biomimetic physiological functionality. In this chapter, the physical parameters related to laser induced forward transfer (LIFT) technique, which is implemented in the LAB, are presented. These parameters, such as laser pulse energy and bio-ink viscosity are critical to control the cell printing process. They must be tuned according to each other in order to print viable cell patterns with respect to celllevel histological organization. Processing time is a concern when addressing tissue engineering involving living material like cells. Therefore, concerns regarding the design and technical implementation of LAB based rapid prototyping workstation are discussed. Experimental requirements are described in order to fabricate tissues using LAB. Some typical multi-component printing, 3D printing approaches and bio-printing in vivo are presented. Laser Assisted Bio-printing for Biomimetic Tissue EngineeringThere are several ways for the technological generation of three-dimensional biological structures. As an alternative to the rather straight forward scaffold-based approach of cell seeding on porous templates [1], some authors have suggested that B. Guillotin · S. Catros · F. Guillemot (B)

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Adipogenic differentiation of laser-printed 3D tissue grafts consisting of human adipose-derived stem cells

Cited by 142 publications

References 42 publications

Laser-based 3D cell printing for tissue engineering

Laser-based 3D cell printing for tissue engineering

3D bioprinting for tissue engineering: Stem cells in hydrogels

Laser Assisted Bio-printing (LAB) of Cells and Bio-materials Based on Laser Induced Forward Transfer (LIFT)

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