Adipocytes secretome from normal and tumor breast favor breast cancer invasion by metabolic reprogramming

Zaoui, Maurice; Morel, Mehdi; Louadj, Lila; Ferrand, Nathalie; Lamazière, Antonin; Uzan, Catherine; Canlorbe, Geoffroy; Atlan, Michaël; Sabbah, Michèle

doi:10.1007/s12094-022-03035-y

Cited by 3 publications

(2 citation statements)

References 42 publications

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“…Adipose tissue, consisting of subcutaneous and visceral depots, plays a role in regulating metabolism and secreting factors that influence cancer. Adipocyte‐derived factors together form a complex mixture known as adipocyte conditioning medium (ACM), which impacts cellular processes 10,11 . These ACM factors contribute to the development of both castration‐sensitive PC (CSPC) and castration‐resistant PC (CRPC), enhancing PC cell migration and invasion, suggesting a potential role of adipocytes in promoting PC progression 12 .…”

Section: Introductionmentioning

confidence: 99%

“…Adipocyte-derived factors together form a complex mixture known as adipocyte conditioning medium (ACM), which impacts cellular processes. 10,11 These ACM factors contribute to the development of both castration-sensitive PC (CSPC) and castration-resistant PC (CRPC), enhancing PC cell migration and invasion, suggesting a potential role of adipocytes in promoting PC progression. 12 While our full understanding of the molecular contributors within ACM that affect PC cells remains partial, various potential factors influencing PC growth and survival have been identified.…”

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confidence: 99%

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Multifaceted impact of adipose conditioned media: Obesity‐driven promotion of prostate cancer and cancer stem cell dynamics

Erdogan,

Serttas,

Dibirdik

et al. 2024

Cell Biochemistry & Function

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Obesity is an established risk factor for the development and progression of prostate cancer (PC). This study used adipose conditioned media (ACM) from differentiated adipocytes to assess its effect on PC development and aggressiveness. Due to limited research on ACM's impact on isolated PC stem cells (PCSCs), we also examined CD44+ PCSCs. ACM notably boosted interleukin‐1β (IL‐1β), IL‐6, and IL‐8 production in normal prostate epithelial cells and LNCaP cells. It also increased IL‐6 and IL‐8 production in PC3 and CD44+ LNCaP cells, and IL‐1β and IL‐6 production in CD44+ PC3 cells. This indicates that ACM induces the production of inflammatory cytokines in both cancer and prostate epithelial cells. Furthermore, ACM promoted proliferation in androgen receptor (AR)‐negative PC3 cells, CD44+ PC3 PCSCs, and nonmalignant RWPE cells, without affecting AR‐positive LNCaP cells. In addition, ACM‐enhanced invasion and migration potential in both PC3 and CD44+ PC3 cells. Western blot analysis indicated the involvement of NF‐κB and AKT pathways in ACM‐induced proliferation in PC3 cells and NF‐κB in PCSCs. In ACM‐treated PC3 cells, E‐cadherin was downregulated, while N‐cadherin, Snail, vimentin, fibronectin, and Twist were upregulated, suggesting ACM‐induced invasion via classical epithelial‐to‐mesenchymal transition (EMT) pathways. In response to ACM, PCSCs exhibited increased expression of E‐cadherin, Snail, and vimentin, which are partial EMT markers promoting stemness and resistance to apoptosis. In addition, increased expressions of Nanog, Oct3/4, survivin, and Bcl‐2 were observed. Although the molecules we studied have diverse effects on cellular regulation, our data emphasize obesity's multifaceted role in promoting and aggressing PC, notably affecting PCSC populations.

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Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

Multifaceted impact of adipose conditioned media: Obesity‐driven promotion of prostate cancer and cancer stem cell dynamics

Erdogan,

Serttas,

Dibirdik

et al. 2024

Cell Biochemistry & Function

View full text Add to dashboard Cite

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Lipid metabolism and its implications in tumor cell plasticity and drug resistance: what we learned thus far?

Vishwa,

BharathwajChetty,

Girisa

et al. 2024

Cancer Metastasis Rev

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High BMI Is Associated with Changes in Peritumor Breast Adipose Tissue That Increase the Invasive Activity of Triple-Negative Breast Cancer Cells

Miracle,

McCallister,

Denning

et al. 2024

IJMS

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Breast cancer is the most common cancer in women with multiple risk factors including smoking, genetics, environmental factors, and obesity. Smoking and obesity are the top two risk factors for the development of breast cancer. The effect of obesity on adipose tissue mediates the pathogenesis of breast cancer in the context of obesity. Triple-negative breast cancer (TNBC) is a breast cancer subtype within which the cells lack estrogen, progesterone, and HER2 receptors. TNBC is the deadliest breast cancer subtype. The 5-year survival rates for patients with TNBC are 8–16% lower than the 5-year survival rates for patients with estrogen-receptor-positive breast tumors. In addition, TNBC patients have early relapse rates (3–5 years after diagnosis). Obesity is associated with an increased risk for TNBC, larger TNBC tumors, and increased breast cancer metastasis compared with lean women. Thus, novel therapeutic approaches are warranted to treat TNBC in the context of obesity. In this paper, we show that peritumor breast adipose-derived secretome (ADS) from patients with a high (>30) BMI is a stronger inducer of TNBC cell invasiveness and JAG1 expression than peritumor breast ADS from patients with low (<30) BMI. These findings indicate that patient BMI-associated changes in peritumor AT induce changes in peritumor ADS, which in turn acts on TNBC cells to stimulate JAG1 expression and cancer cell invasiveness.

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Adipocytes secretome from normal and tumor breast favor breast cancer invasion by metabolic reprogramming

Cited by 3 publications

References 42 publications

Multifaceted impact of adipose conditioned media: Obesity‐driven promotion of prostate cancer and cancer stem cell dynamics

Multifaceted impact of adipose conditioned media: Obesity‐driven promotion of prostate cancer and cancer stem cell dynamics

Lipid metabolism and its implications in tumor cell plasticity and drug resistance: what we learned thus far?

High BMI Is Associated with Changes in Peritumor Breast Adipose Tissue That Increase the Invasive Activity of Triple-Negative Breast Cancer Cells

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