Adipocytes as an Important Source of Serum S100B and Possible Roles of This Protein in Adipose Tissue

Gonçalves, Carlos Alberto; Leite, Marina Concli; Guerra, Maria Cristina

doi:10.1155/2010/790431

Cited by 76 publications

(54 citation statements)

References 76 publications

(83 reference statements)

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“…However, we demonstrated that a non-impact exercise (swimming) also increased serum S100B levels in humans, providing the basis to assume that extra-cerebral sources could additionally release/leak protein into blood (Dietrich et al 2003). For instance, experimental data show that under stress conditions, cardiomyocytes and adipocytes can leak S100B into medium (Mazzini et al 2005(Mazzini et al , 2009Gonçalves et al 2010). Furthermore, S100B protein is strategically located on the skeletal muscle membranes, sarcoplasmic reticulum, and transverse tubules acting in association with other proteins linked with calcium homeostasis (Arcuri et al 2002).…”

Section: Introductionmentioning

confidence: 80%

Serum S100B level increases after running but not cycling exercise

Stocchero

Oses

Cunha

et al. 2014

Appl. Physiol. Nutr. Metab.

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The objective of this study was to investigate the effect of running versus cycling exercises upon serum S100B levels and typical markers of skeletal muscle damage such as creatine kinase (CK), aspartate aminotransferase (AST) and myoglobin (Mb). Although recent work demonstrates that S100B is highly expressed and exerts functional properties in skeletal muscle, there is no previous study that tries to establish a relationship between muscle damage and serum S100B levels after exercise. We conducted a cross-sectional study on 13 male triathletes. They completed 2 submaximal exercise protocols at anaerobic threshold intensity. Running was performed on a treadmill with no inclination (RUN) and cycling (CYC) using a cycle-simulator. Three blood samples were taken before (PRE), immediately after (POST) and 1 h after exercise for CK, AST, Mb and S100B assessments. We found a significant increase in serum S100B levels and muscle damage markers in RUN POST compared with RUN PRE. Comparing groups, POST S100B, CK, AST and Mb serum levels were higher in RUN than CYC. Only in RUN, the area under the curve (AUC) of serum S100B is positively correlated with AUC of CK and Mb. Therefore, immediately after an intense exercise such as running, but not cycling, serum levels of S100B protein increase in parallel with levels of CK, AST and Mb. Additionally, the positive correlation between S100B and CK and Mb points to S100B as an acute biomarker of muscle damage after running exercise.

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Section: Introductionmentioning

confidence: 80%

Serum S100B level increases after running but not cycling exercise

Stocchero

Oses

Cunha

et al. 2014

Appl. Physiol. Nutr. Metab.

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show abstract

“…S100B increments have been reported in many conditions of brain injury and neurodegenerative diseases (Kleindienst et al, 2007;Rothermundt et al, 2003). Serum S100B protein comes from the brain and from other non-neural sources, particularly adipose tissue (Goncalves et al, 2010. In fact, serum S100B levels in humans appear to closely reflect adipose tissue mass (Gross et al, 2010;Steiner et al, 2010a).…”

Section: Discussionmentioning

confidence: 99%

Serum triglycerides, but not cholesterol or leptin, are decreased in suicide attempters with mood disorders

Cantarelli

Nardin

Buffon

et al. 2015

Journal of Affective Disorders

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“…However, when BM-hMSCs were preincubated with ATP and then differentiated to the adipogenic lineage (ATP pretreat adipo), some master genes involved in adipogenesis such as ADIPOQ, a PPARg [52] and CEPBPA [53] target gene, and several adipocyte differentiation markers such as ESM1 [54] and S100B [55] were significantly increased compared to untreated counterpart (untr adipo). Moreover, ID1, a key transcription factor in osteogenic differentiation, was downregulated, as previously reported [56] (Fig.…”

Section: Gene Expression Profile Of Atp-treated Undifferentiated Bm-hmentioning

confidence: 99%

Extracellular Purines Promote the Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells to the Osteogenic and Adipogenic Lineages

Ciciarello

Zini

Rossi

et al. 2013

Stem Cells and Development

101

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Extracellular nucleotides are potent signaling molecules mediating cell-specific biological functions, mostly within the processes of tissue damage and repair and flogosis. We previously demonstrated that adenosine 5¢-triphosphate (ATP) inhibits the proliferation of human bone marrow-derived mesenchymal stem cells (BMhMSCs), while stimulating, in vitro and in vivo, their migration. Here, we investigated the effects of ATP on BM-hMSC differentiation capacity. Molecular analysis showed that ATP treatment modulated the expression of several genes governing adipogenic and osteoblastic (ie, WNT-pathway-related genes) differentiation of MSCs. Functional studies demonstrated that ATP, under specific culture conditions, stimulated adipogenesis by significantly increasing the lipid accumulation and the expression levels of the adipogenic master gene PPARc (peroxisome proliferator-activated receptor-gamma). In addition, ATP stimulated osteogenic differentiation by promoting mineralization and expression of the osteoblast-related gene RUNX2 (runt-related transcription factor 2). Furthermore, we demonstrated that ATP stimulated adipogenesis via its triphosphate form, while osteogenic differentiation was induced by the nucleoside adenosine, resulting from ATP degradation induced by CD39 and CD73 ectonucleotidases expressed on the MSC membrane. The pharmacological profile of P2 purinergic receptors (P2Rs) suggests that adipogenic differentiation is mainly mediated by the engagement of P2Y1 and P2Y4 receptors, while stimulation of the P1R adenosine-specific subtype A2B is involved in adenosine-induced osteogenic differentiation. Thus, we provide new insights into molecular regulation of MSC differentiation.

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Adipocytes as an Important Source of Serum S100B and Possible Roles of This Protein in Adipose Tissue

Cited by 76 publications

References 76 publications

Serum S100B level increases after running but not cycling exercise

Serum S100B level increases after running but not cycling exercise

Serum triglycerides, but not cholesterol or leptin, are decreased in suicide attempters with mood disorders

Extracellular Purines Promote the Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells to the Osteogenic and Adipogenic Lineages

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