Adipocyte β-arrestin-2 is essential for maintaining whole body glucose and energy homeostasis

Pydi, Sai Prasad; Jain, Shanu; Tung, Wesley; Cui, Yinghong; Zhu, Lu; Sakamoto, Wataru; Jain, Shalini; Abel, Brent S.; Skarulis, Monica C.; Liu, Jie; Huynh, Thanh; Pacák, Karel; Caron, Marc G.; Гаврилова, Оксана; Finkel, Toren; Wess, Jürgen

doi:10.1038/s41467-019-11003-4

Cited by 43 publications

(58 citation statements)

References 68 publications

(108 reference statements)

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“…In a previous study, we demonstrated that mice selectively lacking barr2 in adipocytes (adipo-barr2-KO mice) show pronounced improvements in glucose homeostasis (10), a phenotype that is exactly opposite to the one observed in the present study with adipo-barr1-KO mice. We found that these beneficial metabolic effects were primarily due to enhanced signaling via adipocyte 3-adrenergic receptors (10). Thus, the notably different phenotypes of adipo-barr1-KO versus adipo-barr2-KO mutant mice indicate that barr1 and barr2 can have opposing functions in the same cell type in vivo.…”

Section: Discussioncontrasting

confidence: 92%

“…Libraries for RNA-seq studies were prepared as described previously (10). Briefly, total RNA was extracted from BAT of adipo-barr1-KO mice and control littermates (males) that had been maintained on an HFD for 8 weeks.…”

Section: Rna-seq Studiesmentioning

confidence: 99%

“…Indirect calorimetry measurements TEE, respiratory exchange ratio (O 2 consumed/CO 2 produced), food intake, and locomotor activity (assayed by beam breaks) were measured simultaneously in mice housed at 22°C using an Oxymax/ CLAMS (Columbus Instruments). Sampling was performed every Water and food were provided ad libitum (10). For testing 3adrenergic receptor-mediated thermogenesis, mice were maintained at 30°C overnight and then injected intraperitoneally with CL316, 243 [0.1 mg/kg; selective 3-adrenergic receptor agonist (Sigma-Aldrich)] or vehicle (saline) with a crossover treatment performed on the following day.…”

Section: Rna-seq Studiesmentioning

confidence: 99%

“…Western blot analysis was performed as described previously (10). Briefly, tissues per cells were homogenized using adipocyte lysis buffer [50 mM tris (pH 7.4), 500 mM NaCl, 1% NP-40, 20% glycerol, 5 mM EDTA, and 1 mM phenylmethylsulphonyl fluoride], followed by centrifugation at 12,000g for 15 min.…”

Section: Western Blotsmentioning

confidence: 99%

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β-arrestin-1 suppresses myogenic reprogramming of brown fat to maintain euglycemia

et al. 2020

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A better understanding of the signaling pathways regulating adipocyte function is required for the development of new classes of antidiabetic/obesity drugs. We here report that mice lacking β-arrestin-1 (barr1), a cytoplasmic and nuclear signaling protein, selectively in adipocytes showed greatly impaired glucose tolerance and insulin sensitivity when consuming an obesogenic diet. In contrast, transgenic mice overexpressing barr1 in adipocytes were protected against the metabolic deficits caused by a high-calorie diet. Barr1 deficiency led to a myogenic reprogramming of brown adipose tissue (BAT), causing elevated plasma myostatin (Mstn) levels, which in turn led to impaired insulin signaling in multiple peripheral tissues. Additional in vivo studies indicated that barr1-mediated suppression of Mstn expression by BAT is required for maintaining euglycemia. These findings convincingly identify barr1 as a critical regulator of BAT function. Strategies aimed at enhancing barr1 activity in BAT may prove beneficial for the treatment of type 2 diabetes.

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Section: Discussioncontrasting

confidence: 92%

Section: Rna-seq Studiesmentioning

confidence: 99%

Section: Rna-seq Studiesmentioning

confidence: 99%

Section: Western Blotsmentioning

confidence: 99%

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β-arrestin-1 suppresses myogenic reprogramming of brown fat to maintain euglycemia

et al. 2020

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“…13 Meanwhile, β-arrestins can act as signaling molecules which play a critical role in regulating metabolic functions. 14…”

Section: Introductionmentioning

confidence: 99%

<p>The Role of β-Arrestin1 in Esophageal Squamous Cell Carcinoma</p>

Tan

Dong

et al. 2020

OTT

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Introduction: Esophageal squamous cell carcinoma (ESCC) is the predominant type of esophageal carcinoma with a low survival rate and a poor prognosis. Therefore, it is of great significance to explore the effective tumor markers in early diagnosis, treatment monitoring and prognosis evaluation of ESCC. The current study was designed to explore the important role of β-arrestin1 in ESCC and the underlying mechanism. Methods: The defined effects of β-arrestin1 on cell proliferation, migration, invasion, EMT and tumor growth were investigated both in ESCC cells and in vivo model of ESCC. β-arrestin1 expression was detected using Western blot and immunohistochemistry assay. The cell proliferation ability was determined using CCK-8 assay. Wound healing assay and trans-well invasion assay were performed to determine cell migration and invasion. The key proteins related to cell migration, invasion and EMT were detected by Western blot. Tumor growth in vivo was also monitored by tumor volume and weight. In addition, the effects of β-arrestin1 on AKT/GSK3β/ β-catenin pathway were evaluated.Results: β-arrestin1 was aberrantly upregulated in human ESCC tissues, ESCC cell lines and animal model of ESCC. β-arrestin1 downregulation inhibited cell proliferation, migration, invasion and EMT of ESCC in vitro and vivo. β-arrestin downregulation also suppressed tumor growth in vivo model of ESCC. In addition, the inhibitory effects of β-arrestin1 downregulation were exerted via AKT/GSK3β/β-catenin signaling pathway. Discussion: The results in the present study together confirmed the truth that β-arrestin1 interference may suppress ESCC cell proliferation, migration, invasion, EMT and tumor growth via AKT/GSK3β/β-catenin signaling pathway.

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Measuring Brown Fat Using MRI and Implications in the Metabolic Syndrome

Huang

et al. 2020

Magnetic Resonance Imaging

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Metabolic syndrome is presently becoming a global health concern. Brown adipose tissue (BAT) has the potential for managing the risk factors of metabolic syndrome by adjusting plasma lipids and glucose. Magnetic resonance imaging (MRI) is a noninvasive and radiation‐free imaging modality for BAT research and clinical applications in both animals and humans. In the past decade, MRI technologies for detecting and characterizing BAT have developed rapidly, with progress in MRI sequencing and the emerging understanding of BAT. In this review, we focus on the main MRI methods for BAT including currently used imaging techniques and new methods and their implications for the symptoms and complications of metabolic syndrome. Level of Evidence 5 Technical Efficacy Stage 2

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Adipocyte β-arrestin-2 is essential for maintaining whole body glucose and energy homeostasis

Cited by 43 publications

References 68 publications

β-arrestin-1 suppresses myogenic reprogramming of brown fat to maintain euglycemia

β-arrestin-1 suppresses myogenic reprogramming of brown fat to maintain euglycemia

<p>The Role of β-Arrestin1 in Esophageal Squamous Cell Carcinoma</p>

Measuring Brown Fat Using MRI and Implications in the Metabolic Syndrome

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